LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

Serum, as a good assistant in cell culture, plays an important role in the growth process of cells. The quality of serum directly affects the results of cell culture. If there is too much precipitation, it will inevitably cause concern. So, how do we avoid precipitation in   petg media bottles ? First of all, we should have a correct understanding of precipitation. When turbid and flocculent substances are found after thawing the serum, there is no need to panic. This is a normal phenomenon. Common deposits include fibrin, calcium phosphate, cholesterol, fatty acid esters, and some proteins. The amount of serum sedimentation is not directly proportional to the quality of the serum. If serum precipitation occurs, don’t worry. Usually, centrifuge at 400-600g for 5 minutes to remove large particle precipitation, and then use the supernatant. PETG Media Bottles Of course, if you still feel uneasy, you can take the following measures to avoid precipitation in the petg media bottles as m...

  Serum is an essential nutrient when culturing cells. It provides binding proteins, growth factors, hormones, etc. for cell growth, and also protects cells.   PETG media bottle   is a special storage container for this substance. Serum has strict requirements on storage conditions. So, what are the characteristics of its packaging? Low temperature resistance: The storage temperature of serum is -5°C to -20°C, and the lower storage temperature determines the low temperature resistance of its storage container. The material of the PETG media bottle has very good low temperature resistance and can withstand a low temperature of -60°C, which is also a very important performance. Square design: In daily life, most of the plastic bottles we see are round in design, while the storage containers of serum bottles are in square design. This design conforms to ergonomic features, easy to grasp, and more convenient to use. FuDau 125ml Square PETG Media Bottles Sterility: Cell cultur...

FuDau PETG Media Bottles are mainly used for storing and sampling active pharmaceutical ingredients, bulk intermediates, and also for preparing, storing buffers, culture.solutions or long-term storage of pH sensitive liquids. Type: 5/10/30/60/125/250/500/1000ml. Material: PETG Production Environment: C-GMP Sterilization: 60co-γ irradiation SAL=10, Non-Pyrogenic, Dnase & Rnase free. The bottle body is square, easy to hold, easy to store. The surface is smooth and shiny, easy to clean, not easy to leave residue. Good chemical resistence Low temperature resistence: -70 https://www.cellcultureflasks.com/product-class/Square-Media-Bottles1.html

As an essential nutrient for cell growth, serum has a good protective effect on cells in culture, and the quality of serum directly affects the growth state of cells.   PETG media bottle   is a special container for storing serum, it must meet some necessary conditions to ensure the stability and safety of serum during storage. Low temperature resistance: The storage of serum is different from ordinary items, and generally needs to be stored in an environment of -5°C to -20°C. If stored at 4°C, try not to exceed one month. This requires that the media bottle must have good low temperature resistance to meet the low temperature storage requirements of serum. PETG Media Bottles Transparency: The quality of the serum, which we can initially discern by its appearance. A good serum should be transparent and clear, earthy yellow or brownish yellow, with no or very little precipitation, and relatively viscous. If the serum is found to be turbid, opaque, and contains many precipitates...

  Serum is a nutrient required for cell culture, and plays an important role in maintaining osmotic pressure, promoting cell attachment and spreading, and maintaining logarithmic growth of cells. The serum is stored in a special   media bottle , but if the quality of the bottle is not up to standard, it will directly affect the quality of the serum. In general, the quality of serum depends on two factors: one is the object of sampling, and the other is the process of sampling. Animals used for sampling should be healthy and disease-free and within the specified number of days of birth. The sampling process should be strictly in accordance with the operating procedures, and the prepared serum should undergo strict quality identification. Requirements in the "Procedures for the Production of Biological Products Using Animal Cells in Vitro Culture" published by WHO: 1. Bovine serum must be from a herd or country that is documented to be free of BSE. and should have an appropriat...

  PETG media bottle   is a square plastic bottle with a transparent body and a scale. It is square in shape and is mostly used for the storage of serum or various culture media, buffers, and cell cryopreservation solutions. In addition, it can also be used as a storage container for rabbit red blood cells. Rabbit erythrocytes are aseptically collected anticoagulated rabbit blood, centrifuged to obtain erythrocyte precipitates, washed 2-3 times with an equal amount of PBS until the supernatant is clear, and then prepared to the corresponding ratio of rabbit erythrocytes with Aertenberg’s solution. This kind of cells is mainly used for red blood cell indirect agglutination test, complement fixation test, T lymphocyte E rosette test, production of sensitized cells, immune injection of antibodies, production of blood plate and other tests, and can also be used as an additive for the basis of HB4135 Izumi's medium. 30ml PETG Media Bottles The storage temperature of rabbit erythrocy...

  Cell culture plays an important role in the pharmaceutical industry, vaccine development and other biomanufacturing fields. Cell growth requires nutrients such as culture medium, and the basic requirement for these nutrients is the absence of cytotoxicity and other factors that affect cell growth. How to detect the cytotoxicity of the   square media bottles   packaged as the media? Cytotoxicity is the action of chemical substances (drugs) on the basic structure and/or physiological processes of cells, such as cell membrane or cytoskeleton structure, metabolic process of cells, synthesis, degradation or release of cell components or products, ion regulation and cell division, etc. , leading to the disturbance of cell survival, proliferation and/or function, and the adverse reactions caused by it. Cytotoxicity detection of square media bottles is mainly based on the change of cell membrane permeability, and the following methods are commonly used: PETG Square Media Bottle...

PETG media bottle   is a storage container with excellent performance. Thanks to various good properties of the material, it has the characteristics of light weight, non-fragile, high transparency, and low temperature resistance. It is also a commonly used packaging for trypan blue solution. Trypan blue is an organic compound with a chemical formula of C34H24N6Na4O14S4, which is often used as a cell viability dye to detect the integrity of cell membranes and whether cells are alive. Live cells are not stained blue, while dead cells are stained light blue. Trypan blue can be phagocytized by macrophages, so it can be used as a vital stain for macrophages. 60ml PETG Media Bottles Trypan blue solution is mainly for the detection of cell growth and proliferation, it is an important stain that is not absorbed by healthy living cells. When cells are damaged or dead, trypan blue will enter the dead cells, which is convenient for dead cell counting. This method is also sometimes used as a s...

  The square media bottle is a special container for holding the medium. It is generally made of transparent PETG material through injection stretch blowing process. The quality of the bottle is very important for the storage of the medium. So what is the production process of this container? In the production process of the square media bottle, the whole bottle blowing process can be divided into four stages: stretching, pre-blowing, blow molding and cooling. When the temperature adjustment process is completed, the mold is closed, and the stretch rod moves downward to stretch the preform downward along the axis. The lowering speed of the stretching rod is controlled by the stretching cam. The material distribution along the axis of the bottle is formed at this moment. The stretching speed is one of the key parameters affecting the distribution of the material along the axis. If it is too fast, there will be too much material at the bottom of the bottle, resulting in insufficient ...

Serum is a nutrient required for cell growth, among which fetal bovine serum is of better quality. This is because fetal cows have not been in contact with the outside world, and the serum contains few antibodies and complements that are harmful to cells. When using serum, we often have to divide the serum into   PETG media bottles . How to divide it? Fetal bovine serum is a slightly viscous liquid with light yellow and clear appearance, no hemolysis, and no foreign matter. Because of its special use, it is easy to introduce various sources of pollution if you do not pay attention during the packaging process. Contaminated serum is used for cell culture It will affect the normal growth of cells. Therefore, when subpackaging fetal bovine serum, you must follow the prescribed steps: PETG Media Bottles Inactivation: water bath, 56°C, 30 minutes, and shake evenly at any time. Take it out, put it on ice immediately, and let it cool down to room temperature naturally (about 1-3 hours). D...

  PETG is a kind of transparent plastic, which belongs to non-crystalline copolyester. It has good viscosity, transparency, color, chemical resistance, and stress whitening resistance. It can be thermoformed or extrusion blow molded very quickly. It is a good choice for making media bottle of good raw material. PETG raw materials have the following characteristics: 1. Thermoforming performance PETG is easy to produce products with complex shapes and large draw ratios. This material does not need to be pre-dried before thermoforming. The molding cycle is short, the temperature is low, and the yield is higher. 2. Toughness Extruded sheets of PETG are generally 15 to 20 times tougher than general-purpose acrylics and 5 to 10 times tougher than impact-modified acrylics, and have sufficient bearing capacity during processing, transportation and use to help prevent rupture. PETG Media Bottles 3. Weather resistance PETG offers excellent weather resistance. It maintains the firmness of the...

  In biological laboratories, we often have to store various solutions and reagents. Thanks to the advantages of good performance, transparency, and temperature resistance, serum bottles have become "frequent visitors" in the laboratory. As a commonly used storage container, what are its common specifications?                      FuDau 60ml-Square-PETG-PET-Media-Bottles Common materials for serum bottles include PETG and PET, both of which have very good temperature resistance and transparency. In comparison, bottles made of PETG have more advantages in terms of barrier properties. If you want to pursue a better storage state of the solution, it is recommended to use a bottle made of PETG. There is a molded scale on the body of the bottle, we can clearly observe the capacity and state of the solution. The common specifications of this kind of container include 5ml/10ml/30ml/60ml/125ml/250ml/500ml/1000ml, etc., which are mostly...

  Serum is a commonly used nutrient substance when culturing cells, and the quality of serum is directly related to the growth status of cells. Sometimes we will find that there are flocculent sediments in the   square petg media bottles . In fact, there is no need to be too nervous when this happens. These sediments are mainly the following three types of substances: 1. Fibrin: It is a relatively large precipitate that often appears, which can reach 1-2mm and can be observed with the naked eye. Because serum is collected and processed quickly at low temperatures, some fibrinogen (the soluble precursor to flocculent fibrin) remains dissolved during processing and will Fibrin precipitates from clotting in serum bottles. FuDau 125ml Square Petg Media Bottles 2. Calcium phosphate: It is also a common precipitate, which usually makes the serum appear turbid, and it will increase when cultured at 37°C. This deposit looks like tiny black dots under an inverted microscope, which are ...

  Square media bottles are mainly used to hold various culture medium, serum, buffer and other solutions required for cell culture. In view of the strict requirements of cell culture tests on consumable products, this kind of packaging needs to go through various tests to ensure that it is qualified before it can be put into use. . Among many testing items, the hemolysis test is a very important one. Cell hemolysis refers to the phenomenon that the cell membrane bursts outward due to osmotic pressure, quick freezing, mechanical force, biological or chemical toxins, etc., resulting in the outflow of cytoplasmic substances. The hemolysis test in culture media bottles is used to test whether samples contain leachable compounds that would cause hemolysis of red blood cells in vitro. FuDau 125ml Square PETG/PET Media Bottles Refer to ISO10993-4:2017: Biological Evaluation of Medical Devices Part 4: Selection of Tests for Interaction with Blood; GB/T 16886.4-2003 Biological Evaluation of...

  Serum bottle   is a storage container commonly used in laboratories, and is mostly used to store solutions such as serum, culture medium, and various reagents. There are many suppliers of serum bottles on the market, and the product quality is uneven. So, how do we choose this container? It can be considered from the following aspects:                      FuDau 60ml-Square-PETG-PET-Media-Bottles 1. Product material: The materials of serum bottles generally include PET and PETG. The properties of these two materials are similar, and both have good low temperature resistance and transparency. The difference is that the barrier property of PETG is better than that of PET. If you want to pursue a better solution storage effect, it is recommended to choose PETG material. 2. Production process: At present, the production process used for this kind of packaging container is mostly injection blowing and injection stretch blowin...

  PETG media bottle   is a special package for storing various media, reagents, serum and other solutions, and it is also a product that researchers have more contact with. The wide range of applications is mainly due to the superior properties of its materials. PETG is a transparent plastic belonging to the non-crystalline copolyester. Serum needs to be stored in a low temperature environment. The use range of this material is -80°C to 60°C, which makes the PETG media bottle fully meet the storage needs of serum. In addition, it has the following features: 1. High transparency, light transmittance up to 90%, can achieve the transparency of plexiglass;                     FuDau 60ml PETG/PET Square Media Bottles 2. It has stronger stiffness and hardness, excellent scratch resistance, impact resistance and toughness, close to or even higher than polycarbonate (PC), suitable for injection molding, extrusion molding and extrusion ...

  Media bottles   are special containers for storing serum, culture medium, trypsin, reagents and other products. These solutions are generally used for cell culture, so the packaging must meet the requirements of no endotoxin, so as not to affect the normal growth of cells. So how do you remove endotoxins from containers? The chemical composition of endotoxin is lipopolysaccharide, which is one of the main components of the cell wall of Gram-negative bacteria. A live bacterium releases very little endotoxin, but a large amount of endotoxin can be released after death. Because endotoxin can stimulate many kinds of cells and affect the experimental results, the source of endotoxin in the culture medium should be minimized in the experiment, and the packaging container is an important aspect. At present, the serum bottles on the market are mainly made of glass or PET/PETG, and endotoxins can be tightly adhered to the glassware. The common methods in the laboratory cannot complet...

Fetal bovine serum is obtained from caesarean section fetuses, which is of high quality, because the fetuses have not been exposed to the outside world, and the serum contains less antibodies, complements and other components that are harmful to cells. Factors affecting the quality of fetal bovine serum include appearance, technical indicators, blood source,   media bottle   and other four aspects. Appearance: Including the color, clarity, and precipitation of the serum. Generally, domestic fetal bovine serum is waxy yellow, imported high-quality serum is pale yellow, reddish, and slightly orange-red or bright red. Heat-inactivated serum or improperly stored serum will appear dark red or even brown due to the destruction and oxidation of hemoglobin. Technical indicators: Technical indicators affecting serum quality include endotoxin, total protein content, hemoglobin, PH, microorganisms, immunoglobulins, etc., as well as the determination of antibiotics. Domestic dairy cattle ...

  Serum is the most widely used type of cell culture medium, especially high-quality fetal bovine serum. With the rapid development of biomedical technology, the demand for serum is also rising, so what is the future market demand for   media bottles   as serum storage containers? Serum, as a nutrient for cell growth, is mainly used in the early cell culture stage of biopharmaceutical industrial production. Biopharma has been at the forefront of the entire pharmaceutical industry, representing both knowledge-intensive and capital-intensive industries. Since the beginning of the new century, breakthroughs have been made in the research of life science and biomedicine, and biotechnology has become the focus of the world's scientific and technological competition. The global biopharmaceutical industry has developed rapidly in recent years, and a large number of new drugs have passed clinical trials. According to relevant statistics, the world's top ten best-selling drugs and...

Media bottle is a special packaging made of transparent polyester (PET/PETG) raw material with organic solvent added through injection stretch blow production process. In order to facilitate holding, the shape of the bottle is generally square, and the bottle body is marked with a scale. So, what are the main applications of   media bottles ? 1. Serum storage: Storage of serum is an important application of media bottles. Serum is a complex mixture formed by removing fibrinogen from plasma and should be stored at -5°C to -20°C. If stored at 4°C, it should not exceed one month. If one bottle cannot be used up at a time, it is recommended to aseptically aliquot the serum into an appropriate sterilized container and put it back into the freezer. 2. Medium storage and preparation: Medium is an artificially formulated nutrient for the growth and maintenance of microorganisms, plants and animal tissues, generally containing water, nitrogen sources, inorganic salts (including trace elemen...