LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  Cell culture consumables are indispensable experimental tools in the cell culture process. Commonly used consumables include   cell culture flasks , cell culture dishes, and cell culture plates. So what are their respective uses? 1. Cell culture dishes: Cell culture dish is a laboratory vessel used for microorganism or cell culture. It consists of a flat disc-shaped bottom and a cover, usually made of glass or plastic. The materials of culture dishes are basically divided into two categories, mainly plastic and glass. Glass can be used for plant materials, microbial culture and adherent culture of animal cells. The plastic ones may be made of polyethylene, and are disposable or multiple-use. They are suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can be used for the cultivation of plant materials. Cell Culture Dishes 2. Cell culture plates: Cell culture plate can be divided into flat bottoms and round bottoms (U-shaped and V-shaped) a...

  Cell culture is a very important experimental technology, and has become an indispensable research method in the fields of biopharmaceuticals, life sciences, and clinical transplantation. Cell culture must rely on cell consumables to achieve the conditions required for cell growth.   Cell culture flasks   and   cell culture dishes   are two common types. So what is the difference between these two consumables? Cell culture flasks are suitable for long-term culture, subculture, and as seed cells. The bottle mouth is small and the cells are not easy to be polluted. Cell culture dishes are suitable for use in various experiments and temporary culture. The difference between the two lies in the level of safety factor and the number of cultured cells. T25 Flask   It is better to use cell culture dishes for experiments with cells as carriers or objects, because the amount used is relatively small, saving cells, and petri dishes are more convenient for control e...

  Sf9 cells are Spodoptera frugiperda cells, which are usually used to produce various medicinal proteins and viruses, or for basic research. When culturing sf9 cells, the commonly used cell culture consumables are   cell culture flasks . Sf9 cells are derived from the Insect Pathology Laboratory of the United States Department of Agriculture and are derived from the IPLBSF-21 cell line. The cells are regular with particles and can be cultured in suspension or adherent culture. This cell has a high expression level (especially intracellular proteins), has a faster growth rate, effective protein folding, glycosylation is similar to mammalian cells, and relatively easy enzymatic deglycosylation (for protein structure determination) Favorable), is widely used in the expression of V LPs. Sf-9 cells are semi-adherent cell lines. In the medium containing 10% fetal calf serum, most of the cells usually grow adherently, and a small part is suspended. Grow in a 27°C cell incubator with...

  Cell culture consumables are indispensable experimental tools in the cell culture process. Commonly used consumables include   cell culture flasks , cell culture dishes, and cell culture plates. So what are their respective uses? 1. Cell culture dishes: Cell culture dish is a laboratory vessel used for microorganism or cell culture. It consists of a flat disc-shaped bottom and a cover, usually made of glass or plastic. The materials of culture dishes are basically divided into two categories, mainly plastic and glass. Glass can be used for plant materials, microbial culture and adherent culture of animal cells. The plastic ones may be made of polyethylene, and are disposable or multiple-use. They are suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can be used for the cultivation of plant materials. Cell Culture Dishes 2. Cell culture plates: Cell culture plate can be divided into flat bottoms and round bottoms (U-shaped and V-shaped) a...

  Adherent cells are relatively common cell types. The growth of this type of cells must have a support surface that can be attached, and the cells can grow and reproduce on the surface by relying on the attachment factors secreted by themselves or provided in the medium. Commonly used cell culture consumables for culturing adherent cells include   cell factory systems , cell culture roller bottles, and cell culture flasks. Cell factory systems: Cell culture consumables commonly used in large-scale culture, consisting of one or more layers of structure, common ones are 1 layer, 2 layers, 5 layers, 10 layers, 40 layers, etc., and the layers are connected by ultrasonic welding process , the strength of its junction is close to that of a whole piece of continuous material, which can achieve good sealing. Cell Factory Systems Cell culture roller bottles: a high-quality consumable that can meet the requirements for large-scale production of cells and tissues in both experiments and...

As a convenient cell culture container,   cell culture flasks   appear frequently in laboratories and are mainly used to cultivate adherent cells. When culturing cells, we need to observe the state of the cells and judge their growth. So, how should we observe? First of all, the state of the cells can be observed through eyes or a microscope. When we take out the cell culture flasks from the incubator, we can judge the growth of the cells by the color, concentration, and cell state of the medium. T25 Flask 1. Look at the color of the medium. There are acid-base indicators in most media, which are yellow when acidic and purple when alkaline. If the pH value of the medium changes greatly, consider whether the medium is contaminated, or the cells grow too densely, or the cells die too much, or there is a problem with the adjustment of the CO2 concentration in the incubator. 2. Look at the concentration of the medium. When the medium becomes turbid, the medium is contaminated or t...

  FuDau will participate in the LABVOLUTION 2023 Time: 09 - 11 May 2023 Location: Hannover, Germany Fudau Booth No.: Hall 19, Stand D82/1 LUOYANG FUDAU BIOTECH CO.LTD was established in 2014. It is a professional enterprise integrating R&D, production and sales of biomedical packaging, industrial high-end cell culture consumables, high-end laboratory consumables, and diagnostic consumables. The main products include cell factory, cell culture flasks, cell culture erlenmeyer flasks, cell roller bottles,  PETG media bottles  etc.  PETG Media Bottles Fudau Bio has C-level and D-level purification workshops and C-level microbial testing rooms that meet GMP requirements, and is equipped with domestic leading cell factories and fully automatic production lines for cell culture bottles. Relying on the ISO13485 quality management system, mature production technology and professional production equipment, while realizing the scale and model of biological consumables, Fuda...

Apr. 17, 2023, the U.S. Food and Drug Administration approved Omisirge (omidubicel-onlv), a substantially modified allogeneic (donor) cord blood-based cell therapy to quicken the recovery of neutrophils (a subset of white blood cells) in the body and reduce the risk of infection. The product is intended for use in adults and pediatric patients 12 years and older with blood cancers planned for umbilical cord blood transplantation following a myeloablative conditioning regimen (treatment such as radiation or chemotherapy).  “Today’s approval is an important advance in cell therapy treatment in patients with blood cancers,” said Peter Marks, M.D., Ph.D., director of the FDA’s Center for Biologics Evaluation and Research. “Hastening the return of the body’s white blood cells can reduce the possibility of serious or overwhelming infection associated with stem cell transplantation. This approval reflects the FDA’s continued commitment to supporting development of innovative therapies for...

  When conducting cell culture experiments,   cell culture flasks   are a tool that we often use. Its specifications range from 25cm² to 225cm², which can meet the needs of different scales of cell culture. As a common tool, this consumable has the following three characteristics: 1. Torticollar design: The neck of the cell culture flask adopts a torticollar design, which makes it easier for pipettes and cell scrapers to enter the interior, and there is no dead angle in contact, which is convenient for researchers to operate. 2. Two kinds of covers: According to the different culture environment, this consumable has two kinds of covers, airtight cover and air-permeable cover. Caps are often used for closed cultures, but can also be used for open cultures when the caps are unscrewed. The breathable cover is suitable for open culture. The cover is equipped with a 0.2um hydrophobic filter membrane to avoid possible cross-infection during gas exchange. It is generally recomme...

  Cell culture flasks   are mostly used for the cultivation of adherent cells. Different from the growth of suspension cells in the culture medium, adherent cells need to grow on the surface of the container, so the cells must be digested before harvesting or passage. There are many ways to digest cells, the common ones are as follows: 1. Enzyme digestion This is also a method that we use more in our daily life. Generally, trypsin is used for digestion, and the concentration is between 0.25% and 0.5%. The digestion time varies according to the cell type, operation method, temperature and other factors. After digestion, it is necessary to stop the digestion with serum or serum-containing medium. T25 Flask 2. Physical method Without the aid of trypsin, directly pipette or use a cell scraper to digest the cells on the surface of the cell culture flask. This method can be used for cells that are firmly attached. However, it should be noted that pipetting and scraping will cause ce...

  There are thousands of cell types, but they can be roughly divided into two categories: adherent cells and suspension cells. Different cells have different requirements for culture containers. As a consumable product with high frequency of use,   cell culture flasks   are divided into two types: TC-treated surface and non-TC-treated surface. So, what is the difference between these two surfaces? Cells are divided into adherent cells and suspension cells according to different culture methods. The growth of adherent cells must have a support surface that can be attached, and the cells can grow on the surface by relying on the attachment factors secreted by themselves or provided in the medium and reproduce. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes. T225 Flask The cell culture flask is made of polystyrene raw material. Compared with glass material, the surface of this material is hydrophobic, which is ...

  Cell culture flasks   are mainly used for adherent cell culture, and cell culture scale-up in laboratory or industrial production processes. According to the different culture conditions, this cell culture container has two types of lids: Sealed Cap and Vent Cap. So, what is the difference between these two types of caps?            FuDau T25 Cell Culture Flasks The sealing cap is completely sealed and is suitable for cell and tissue culture under sealed conditions, so that the culture environment is completely isolated from the outside world. The breathable cover has a hydrophobic filter membrane with a pore size of 0.22um, which can meet the needs of gas exchange in cell and tissue culture, and can effectively prevent cross-contamination. It is suitable for open culture conditions. The two types of caps for cell culture flasks are first used in different environments. The vent cap is suitable for use in an environment with carbon dioxide, whil...

  There are thousands of cell types, but they can be roughly divided into two categories: adherent cells and suspension cells. Different cells have different requirements for culture containers. As a consumable product with high frequency of use,   cell culture flasks   are divided into two types: TC-treated surface and non-TC-treated surface. So, what is the difference between these two surfaces? Cells are divided into adherent cells and suspension cells according to different culture methods. The growth of adherent cells must have a support surface that can be attached, and the cells can grow on the surface by relying on the attachment factors secreted by themselves or provided in the medium and reproduce. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes. T225 Flask The cell culture flask is made of polystyrene raw material. Compared with glass material, the surface of this material is hydrophobic, which is ...

The previous article introduced four of the common pollution types when using cell culture consumables such as   cell culture flasks   for cell culture. Next, I will introduce the remaining three pollution types and their characteristics: 5. Protozoan pollution: The culture medium may be slightly turbid, and the number of tiny dots under the microscope is very large and slightly mobile. Although the cells can grow, the reproduction speed is obviously slowed down, and the cell state is not good, the edges are not clear, and the cells are not clear. Translucent. They can symbiosis with cells but will compete with cells for nutrients. This symbiosis is very common, but their number is small, and the cell site has an advantage, so it will not affect the normal growth of cells, only when they reach a certain number It will affect the growth of cells and eventually form a vicious circle.      T75 Cell Culture Flasks 6. Virus contamination: In the process of tissue cel...

  Cell culture flasks   are commonly used tools for adherent cell culture, and cell subculture operations are involved in culturing cells. How should we passage adherent cells? You can follow these steps: 1. Take out the preheated culture solution: take out the preheated culture solution, wipe it with an alcohol cotton ball before putting it into the ultra-clean bench. 2. Take out the cells from the incubator: Pay attention to tighten the bottle cap when taking out the cells from the cell culture bottle, wipe the microscope table with alcohol cotton balls, and then observe the cells under the microscope. 3. Put the culture bottle into the ultra-clean workbench and open the mouth of the bottle, and at the same time burn the mouth on the alcohol lamp for disinfection. 4. Add digestive solution: Carefully suck out the old culture solution, wash (rinse) with PBS, add appropriate amount of digestive solution (trypsin solution), pay attention to the amount of digestive solution to c...

  Protein expression refers to a molecular biology technology that expresses foreign gene proteins by model organisms such as bacteria, yeast, animal cells or plant cells, and occupies a core position in genetic engineering technology.   Erlenmeyer shake flask   is a consumable often used in protein expression. Protein expression is the process in which genes on DNA are transcribed into RNA and then translated into proteins. Protein expression can express the corresponding (genetic) traits controlled by genes. According to the host cells, protein expression systems can be divided into two categories: prokaryotic expression systems and eukaryotic expression systems. Prokaryotic expression systems include Escherichia coli expression system, Bacillus expression system, Streptomyces expression system. Eukaryotic expression systems include yeast expression systems, insect expression systems, mammalian expression systems and plant cell expression systems.       ...

  The   erlenmeyer shake flask   is a triangular cell culture container. It has two different materials: PC and PETG. According to different uses, there are also two types of lids: vent Cap and sealed Cap. Today, we will introduce the two basic functions of this bottle. Cultivation of suspension cells and fungi: The erlenmeyer shake flask is suitable for cultivating cell lines with high requirements for central enterprises. It is also used to cultivate bacteria, fungi and animal and plant cells in suspension. Compared with culture flasks, dishes and spinner flasks, the required It requires less work and is an affordable cell culture tool. It can be used in small-scale process development, step-by-step scale-up and other cultivation stages. The breathable cover has a 0.2μm breathable membrane, which is breathable and impermeable to water, which can effectively prevent the entry of microorganisms, prevent pollution, and ensure gas exchange, so that cells grow well.   ...

  When culturing cells,   cell culture flasks   are an economical and practical culture tool. So what material is it made of? Cell culture flasks generally use highly transparent polystyrene as raw materials. In order to ensure the safety of raw materials, they must meet USP Class VI medical grade standards. The USP class of medical materials is grade 6, ranging from USP class I-USP class VI, with USP class VI being the highest grade. According to USP-NF general rules, plastics subjected to in vivo biological response tests will be classified into designated medical plastic grades. The purpose of the test is to determine the biocompatibility of plastic products and whether they are suitable for medical device implants and other systems.  FuDau T225 Cell Culture Flasks A plastic that is rated as U.S. Medical Plastics Class VI means that comprehensive and rigorous testing has been established. U.S. medical grade VI is now the gold standard for various medical-grade raw...