Cytotoxicity Test Method for Square PETG Media Bottles

 Cell culture plays an important role in the pharmaceutical industry, vaccine development and other biomanufacturing fields. Cell growth requires nutrients such as culture medium, and the basic requirement for these nutrients is the absence of cytotoxicity and other factors that affect cell growth. How to detect the cytotoxicity of the square media bottles packaged as the media?

Cytotoxicity is the action of chemical substances (drugs) on the basic structure and/or physiological processes of cells, such as cell membrane or cytoskeleton structure, metabolic process of cells, synthesis, degradation or release of cell components or products, ion regulation and cell division, etc. , leading to the disturbance of cell survival, proliferation and/or function, and the adverse reactions caused by it.

Cytotoxicity detection of square media bottles is mainly based on the change of cell membrane permeability, and the following methods are commonly used:

PETG Square Media Bottles

1. MTT and XTT methods: Using the activity of mitochondrial internal enzymes, specific tetrazolium salts can be converted, and then detected by a microplate reader.

2. The method of LDH: the cytotoxicity is detected by detecting the enzyme activity of LDH in the cell culture supernatant.

3. Other enzyme methods: such as detecting the activity of alkaline phosphatase and acid phosphatase in the supernatant.

The above are several detection methods of cytotoxicity. In addition to meeting the requirements of non-cytotoxicity, the square media bottle also needs to meet the requirements of no DNase and no RNase to ensure a good environment for cell growth.