LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  When we use   cell factories   to culture cells, the growth status of the cells is affected by various factors. These factors may seem inconspicuous, but if not paid attention to, they will cause heavy damage to the cells. So, how do small details when using cell factories affect cell growth, and how can we avoid pitfalls when using cell factories to culture cells? 1. Degree of contact between cells Normal cells (diploids) have attachment contact inhibition, and cell growth stops after overgrowth. However, abnormal cells such as tumor cells have weak contact inhibition and can grow to higher cell densities, but some cells overgrow. The damage is difficult to reverse, so it is not recommended to have too high a cell density when culturing cells. Important points: Generally, cells cultured in a cell factory can be passaged when the cell confluence reaches 70-80%. Do not overgrow the cells. Cell Factory Systems 2. Cell growth attachments Adherent cells will adhere to the w...

  The   cell factory   plays an important role in the large-scale cultivation of cells, and has low risk of contamination. It can utilize the maximum cultivation area in a limited space, save a lot of factory space, and reduce the cost of quality control and downstream purification of the enterprise. cost advantages. This kind of culture consumables generally needs surface treatment before it can be used for cell culture. Why? According to different growth modes, cells are divided into adherent cells and suspension cells. The growth of such cells must have a support surface that can be attached, and the cells rely on the attachment factors secreted by themselves or provided in the medium to be on the surface. Growth, propagation, and suspension cells are not required. The cell factory is made of polystyrene raw material, which itself is hydrophobic, which is not conducive to the growth of cell attachment. Therefore, it needs to be modified on the surface to become hydroph...

  Cell factory   is a common cell consumable for large-scale cell culture. It is mainly used in the vaccine industry, cell gene therapy and stem cell fields. So how does the cell factory help the efficient and stable production of vaccines? The industrial production of vaccines is extremely challenging. In the process, cell expansion is involved. Given the complexity of the production process, how to safely and stably scale up has become the focus of the industry. The principle of cell culture expansion is that large-scale culture and small-scale culture maintain a relatively stable environment, and only in the same environment can cell growth density, viability and product expression remain stable. A cell factory is a delicately designed cell culture device, which takes up less space, reduces pollution, increases yield, and has good controllability. It is accepted by more and more biopharmaceutical manufacturers and scientific research institutions. The cell factory realizes ...

  Mycoplasma contamination of cells is a very troublesome problem when culturing cells, especially in the use of large-scale culture vessels such as   cell factory . Once mycoplasma contamination occurs, the loss will be even greater. So, what is the cause of mycoplasma contamination? Mycoplasma is a prokaryotic organism with a size of only 0.2-0.3 um, no cell wall, and can pass through a general filter membrane (0.22-0.45 um). During cell culture, the incidence of mycoplasma infection reaches 63%, so during cell culture Contamination by mycoplasma is a worldwide problem. When cells (especially passage cells) are contaminated by mycoplasma, the expression of DNA, RNA and protein in the cells will change, but the growth rate of cells generally does not have a significant impact, so the contamination of cells by mycoplasma is generally difficult to detect. In most cases, the cytopathic changes are slight or insignificant, and subtle changes can also be alleviated by subculture a...

Today, I will tell you about the application of   cell factories   on Vero cells. Vero cells are derived from African green monkey kidney epithelial cells and are a vaccine production cell line approved by the World Health Organization and my country's biological product regulations. When the cell factory is cultivating Vero cells, our operation method can be expanded from the square flask to the cell factory according to the cell recovery. A 5-layer cell factory is inoculated with about 300x106cells (according to 9.5x104cells/c ㎡ ), adding 200-300ml/ layer, cultured for 3 days, and passaged after the cells grew into a dense monolayer.  FuDau 10 Layers Cell Factory When we pass on the cell factory, we should slowly discard the culture medium in the cell factory, but do not add PBS for rinsing. Add approximately 25 ml/layer of trypsin (Gibco TrypLE™ Express Enzyme (1X)) solution directly. After fully infiltrating the cells for 1-2 min, the trypsin was discarded, and the ce...

  As a large-scale culture vessel,   cell factories   play an important role in the production of products such as human vaccines, animal vaccines, and cell and gene therapy. In the small test stage of the product, a single cell factory is generally the main product, and a larger-scale cultivation area is required to enter the pilot test and batch production stage. At this time, how to quickly scale up on the basis of ensuring safe, consistent, and pollution-free operations has become a key consideration. The cell factory is designed with a multi-layer structure, and scale-up can be done by simply increasing its number. When its number increases, it means that the number of openings increases, not only the operation time will be prolonged, but the risk of contamination will also increase. At this time, we can use a simple way to connect individual factories in series to form a closed culture system. The accessories that need to be used include large-mouth gas conversion k...

  There are four things to keep in mind when using   cell factories   to grow Vero cells: (1) Ensure that the source of cells is reliable, and detect the presence or absence of mycoplasma and foreign virus contamination. (2) The number of consecutive cell passages is limited, and low-passage cells should be used for experiments. FuDau 5 Layers Cell Factory (3) Use a new cell factory for each passage. And in the cell factory culture process abnormal handling (1) The seed cells should be passaged as separately as possible during the culture process. (2) In case of turbidity of the culture medium during cell culture, please select a room where cell experiments will not be performed on that day for sampling and microscopic examination. Microorganisms with obvious swimming under the microscope are likely to be bacterial contamination. If it is not particularly obvious, some culture fluids can be taken Incubate in TSA plate for 24-72h and observe.         Fu...

In the large-scale culture of cells, in order to facilitate the operation, multiple  cell factories  are usually connected in series, which saves the operation of multiple openings and reduces the risk of contamination. So, how do we cascade multiple factories? The cell factory is a culture vessel with a multi-layer structure. Enlarging the culture scale can be achieved by increasing the number of layers. However, when the number of cells increases, we will encounter a problem - repeated repeated operations. As the number of cap openings increases, not only does the operation time increase, but so does the risk of cell contamination. At this time, the developed culture components can be combined into a closed system by connecting multiple containers in series. The operation method is as follows:                 FuDau 10 Layers Cell Factory 1. Prepare custom transfer caps, culture medium, cell factory, and connecting tubing. 2. Connect th...

  Cell factory   play an important role in vaccine production, monoclonal antibodies and the pharmaceutical industry, and are mostly used for the culture of adherent cells. Compared with other cell culture vessels, it has the following advantages: Small footprint: The cell factory adopts a multi-layer structure design, also known as a multi-layer cell incubator. Common specifications include 1 layer, 2 layers, 5 layers, 10 layers, 40 layers, etc. To expand the culture scale, you only need to increase the number of layers of the product That is, it will not take up too much space, which can save a lot of plant space for enterprises, and reduce the cost of quality control and downstream purification of their enterprises. Reduce pollution: This container is equipped with a full set of accessories, including liquid transfer cover, small port conversion cover, CPC adapter, tee pipeline, silicone tube/hot melt tube, ECS quick connector, etc. Through the mutual cooperation of differe...

  When we use cell culture consumables such as   cell factories   to cultivate cells, we may find that there is a lot of cell contamination, which are: bacterial contamination, fungal contamination, mold contamination, and mycoplasma contamination. Bacterial contamination is generally caused by Bacillus and Staphylococcus. The main feature of contamination is that the medium in cell culture consumables such as cell factories turns yellow and turbid, and under low magnification, the medium is sandy and filled with intercellular spaces or culture medium, which can be seen under high magnification. Rod- or globular-shaped bacteria swim in the medium (notably different from the Brownian motion of cell debris). It should be noted that in the early stage of pollution, bacteria often exist in groups, and there may not be obvious movements. At this time, vigilance should be aroused. FuDau 10 Layers Cell Factory The common fungal contamination is Candida albicans contamination. Af...

  Cell factories   are suitable for industrial batch production, laboratory operations, and large-scale cell culture, and are ideal for adherent cells. As a cell culture consumable, what is its production process and how to control its quality? 1. Raw materials: High-quality raw materials are the basis for ensuring products. The cell factory uses USP Class VI compliant polystyrene to ensure batch-to-batch quality stability and consistency. 2. Injection molding: This consumable is produced by injection molding process, and is produced in a special 10,000-level clean room. After rigorous verification (planktonic bacteria, sedimentation bacteria, and suspended particle detection),  q uality management is carried out in accordance with the GMP workshop to ensure stable product quality. 3. Surface treatment: The polystyrene material itself is hydrophobic, so it must undergo surface treatment and introduce hydrophilic groups in order to better adapt to the growth of adherent ce...

  Cell factory   play an important role in vaccine production, monoclonal antibodies and the pharmaceutical industry, and are mostly used for the culture of adherent cells. Compared with other cell culture vessels, it has the following advantages: Small footprint: The cell factory adopts a multi-layer structure design, also known as a multi-layer cell incubator. Common specifications include 1 layer, 2 layers, 5 layers, 10 layers, 40 layers, etc. To expand the culture scale, you only need to increase the number of layers of the product That is, it will not take up too much space, which can save a lot of plant space for enterprises, and reduce the cost of quality control and downstream purification of their enterprises. Reduce pollution: This container is equipped with a full set of accessories, including liquid transfer cover, small port conversion cover, CPC adapter, tee pipeline, silicone tube/hot melt tube, ECS quick connector, etc. Through the mutual cooperation of differe...

  The   cell factory   is a multi-layered cell culture vessel, which is made of polystyrene (PS) raw materials through an injection molding process, and is mainly used to culture adherent cells. The culture container has a novel structure, which can save space and at the same time reduce the quality control cost of the enterprise. Common application fields of cell factories include vaccine production, monoclonal antibody and pharmaceutical industry, etc. The production process of products in these fields is complex, especially in the cell culture stage, which involves procedures such as liquid exchange-culturing, rehydration-culturing, etc. It is very easy to introduce into various This kind of pollution will lead to the reduction of production efficiency or even the stagnation of production, which will bring certain economic losses to the relevant enterprises.  FuDau 10 Layers Cell Factory This multi-layered cell culture container has common specifications including...

  Cell factories   are a type of consumables that are widely used in large-scale cell culture. Cells are very fragile organisms. The in vitro culture of cells requires a specific environment, of which sterility is the basic requirement. In vivo, the detoxification system and immune system can resist the invasion of microorganisms or other harmful substances, but in the process of in vitro culture, cells lack the protection of the body's immune system and lose the ability to defend against microorganisms and detoxify harmful substances. To ensure that cells can grow and reproduce in vitro, it is necessary to ensure a sterile work area, good personal hygiene, sterile reagents and media, and aseptic handling. Common microbial contaminations in cell factory culture include mycoplasma, bacteria, and fungi. Mycoplasma has no lethal toxicity, can coexist with cells for a long time, and has potential effects on cells, but it is small in size and difficult to identify. It can be detect...

  When culturing cells using a   cell factory , it is susceptible to various factors. Such as temperature, PH value, gas environment, etc., of which osmotic pressure is also a very important aspect. Cells in hypertonic or hypotonic solutions can shrink or swell and rupture immediately. Therefore, osmotic pressure is one of the important conditions for culturing cells in vitro. The maintenance of osmotic pressure of mammalian and other animal tissue cells in vitro is mainly related to NaCl, but the relationship of osmotic pressure of other dielectrics cannot be ignored. The osmotic pressure is proportional to the number of molecules and ions of the solute per unit volume of solvent. For this reason, it is very important to control the ion balance in the culture medium in a certain proportion and maintain normal osmotic pressure. This is not only to maintain cell tension, but also to regulate the metabolism of cells. Because extracellular ion transport and ion concentration chan...

  Injection molding is a common processing method for various polymer materials, from plastic trinkets, toys, to auto parts, bottles, containers, and mobile phone casings. This processing method is used in all walks of life. The   cell factory   used in large-scale cell culture is also produced by the injection molding process. The injection molding process is to use an injection molding machine to heat, plasticize and melt the plastic, and then inject it into the cavity of the molding mold for molding. After cooling, the melt is solidified and then demolded. This processing method has the following advantages: 1. It is suitable for the production of complex precision products. The injection molding process can easily design complex plastic precision parts and assemblies. Compared to other techniques, injection molding will find features with very small tolerances. 2. Reduce waste: all the parisons made by injection can be blown into the blow mold, and no waste is generat...

  Cell factory   culture technology plays an important role in the fields of monoclonal antibody and vaccine preparation. When culturing cells, it will be affected by various factors, among which the cell inoculation density will also affect the cell culture process. When using cell factories for large-scale cell culture, the number of cell inoculations has a direct impact on the growth of cells. An appropriate inoculation density can promote better cell proliferation. Too high or too low inoculation density is not conducive to cell growth and proliferation. How to choose the appropriate inoculation concentration should be determined according to the cell metabolism, growth and reproduction speed and the needs of the work. Generally speaking, cells with strong metabolism and fast growth (such as tumor cells) should be inoculated at a lower concentration; while normal tissue cells grow slowly and their metabolism is not vigorous enough, the inoculation concentration may be high...

  The   cell factory   is a multi-layered cell culture consumable. When culturing cells, different experimental operations are often performed with the help of various accessories. Platinum vulcanized silicone tube is a kind of supporting pipeline commonly used in the test process. Platinum vulcanized silicone tube is a silicone tube vulcanized with platinum catalyst. It is mainly used for the transfer of liquid in the cell factory. This kind of pipeline has the characteristics of odorless, non-toxic, acid and alkali resistance, ozone resistance and chemical corrosion resistance. Its processing technology is complex and the production cost is high. It is widely used in food and medical care, and belongs to the relatively high-grade level of silicone tubes. Platinum vulcanization is a two-component addition type vulcanizing agent. It has the characteristics of low vulcanization temperature and fast vulcanization speed. Compared with the traditional peroxide vulcanization s...

  A   cell factory   is a multi-layered cell culture consumable that is generally used for large-scale culture of adherent cells. The quality of consumables directly affects the growth state of cells, so how to control their quality? 1. Raw materials: The raw materials of the cell factory are generally polystyrene, and the raw materials must meet the USP Class VI standard. USPVI level medical equipment test is a relatively strict test for the application of plastic materials in the medical field and pipeline products in biopharmaceuticals. It is a non-clinical laboratory study that meets various experimental specifications. 2. Production environment: cells are particularly sensitive to the growth environment, so consumables are required to contain no substances harmful to cells such as endotoxin, which puts forward higher requirements for their production environment. Consumables must be produced in a dedicated 10,000-class clean room, and undergo rigorous verification (p...