LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  November 09, 2023, the U.S. Food and Drug Administration approved Ixchiq, the first chikungunya vaccine. Ixchiq is approved for individuals 18 years of age and older who are at increased risk of exposure to chikungunya virus. The chikungunya virus is primarily transmitted to people through the bite of an infected mosquito. Chikungunya is an emerging global health threat with at least 5 million cases of chikungunya virus infection reported during the past 15 years. The highest risk of infection is in tropical and subtropical regions of Africa, Southeast Asia, and parts of the Americas where chikungunya virus-carrying mosquitos are endemic. However, chikungunya virus has spread to new geographical areas causing a rise in global prevalence of the disease.  The most common symptoms of chikungunya include fever and joint pain. Other symptoms may include a rash, headache, and muscle pain. Some individuals may experience debilitating joint pain that persists for months or even year...

  In suspension cell culture,   cell culture erlenmeyer flask   is commonly used as a cell culture consumable. The growth of suspension cells does not rely on the surface of the support and grows in a suspended state in the culture medium. In actual culture, how do we determine the amount of liquid to add? The common specifications of cell culture erlenmeyer flask include 125ml, 250ml, 500ml, and 1000ml, which meet the needs of different scales of cell culture. For example, 125ml and 250ml small-capacity bottles are mainly used for small-scale experiments, while 500ml and 1000ml specifications are used for medium-scale cell culture experiments. When this consumable is used, the vibration of the shaker is used to reduce the clumping rate of cells and maintain a good growth state of cells. Cell culture needs to be carried out in a sterile environment. Therefore, the triangular culture flask will undergo special sterilization treatment before it is put into use to achieve th...

  As an economical and affordable cell culture tool, the   erlenmeyer shake flask   has a wide range of applications in the fields of biopharmaceuticals, vaccine preparation, cell and gene therapy, etc. Among them, this kind of container is also used in lentivirus transfection. Lentiviral vectors are gene therapy vectors developed on the basis of HIV-1. Different from general retroviral vectors, it has the ability to infect both dividing and non-dividing cells, and is widely used in the study of expressing RNAi. The cell shake flask is mainly used in the experiment of lentivirus transfection of suspension cells. The steps are as follows:                   125ml-Erlenmeyer-Shake-Flasks 1. Add polybrene to 6 μg/ml and an appropriate amount of virus to 2×105/ml suspended cells, and mix well. Incubate at 37°C. Or centrifuge at room temperature at 150g for 4 hours (selected, this step can improve transfection efficiency for some dif...

  Protein expression refers to a molecular biology technology that expresses foreign gene proteins by model organisms such as bacteria, yeast, animal cells or plant cells, and occupies a core position in genetic engineering technology.   Erlenmeyer shake flask   is a consumable often used in protein expression. Protein expression is the process in which genes on DNA are transcribed into RNA and then translated into proteins. Protein expression can express the corresponding (genetic) traits controlled by genes. According to the host cells, protein expression systems can be divided into two categories: prokaryotic expression systems and eukaryotic expression systems. Prokaryotic expression systems include Escherichia coli expression system, Bacillus expression system, Streptomyces expression system. Eukaryotic expression systems include yeast expression systems, insect expression systems, mammalian expression systems and plant cell expression systems.       ...

  Erlenmeyer shake flask   is a kind of cell consumable that is often used in suspension cell culture. Unlike adherent cells, suspension cells grow in suspension in culture medium. In addition to the gas environment, pH, osmotic pressure and other conditions, the speed of the shaker also affects the growth of cells. Cell culture requires a suitable growth environment and growth temperature, which requires the selection of a suitable shaker for assistance. The constant temperature shaker is a special equipment used in conjunction with the cell shaker. When culturing cells, the shaker needs to be continuously rotated to provide gas conditions for the cells, and at the same time, it can also avoid cell death caused by cell deposition.           FuDau 125ml Erlenmeyer Shake Flasks  The specifications of erlenmeyer shake flasks include 125ml, 250ml, 500ml, 1000ml, 3L, 5L, etc. The volume of medium added to the flasks of different specifications is also...

  Materials and Methods 3.1. Shake Flask Cultivations Pre-cultures of MDCK.SUS2 suspension cells were grown in  shaker flasks  (125 mL polycarbonate Erlenmeyer flasks, #431143, Corning®, New York city, NY, USA) with 50 mL working volume (wv), in a Multitron Pro incubator (Infors HT, Bottmingen, Switzerland) at 37 °C and 5% CO2 atmosphere with a shaking frequency of 180 rpm. Cells were passaged every 3–4 days with a seeding density of 0.5–0.8 × 106 cells/mL. IAV A/Puerto Rico/8/34 (H1N1) seed virus was used for infection, generated in adherent MDCK cells (ECACC # 84121903). The seed virus had a titer of 1.1 × 109 TCID50/mL. Cells were cultivated in a chemically defined, protein-free and animal component-free medium, Smif8 (Smif8 PGD 2×, supplemented with 5 mM glutamine, and 8 mM pyruvate), specifically developed for the cultivation of suspension MDCK cells [95]. Growth and infection experiments for model validation were performed using 500 mL shaking flasks (#4113-0500, Na...

  High-efficiency erlenmeyer shake flasks   are common consumables in cell culture. They are mainly used for the cultivation of mammalian cells, insect cells, yeast cells, fungal cells and other suspension cells. Polycarbonate (PC) is a commonly used raw material for processing. High-efficiency erlenmeyer shake flasks need to observe the growth status of cells in real time when culturing cells, so the transparency of PC material is relatively high. In addition, this material has the following characteristics: 1. Mechanical properties: high strength, fatigue resistance, dimensional stability, and small creep (very little change under high temperature conditions); 2. Heat aging resistance: the enhanced UL temperature index reaches 120~140℃ (long-term outdoor aging resistance is also very good); 3. Solvent resistance: no stress cracking; 4. Stability to water: it is easy to decompose in water at high temperature (use caution in high temperature and high humidity environment); 5. ...

  Erlenmeyer shake flasks   are an important cell culture consumable. They are divided into triangular erlenmeyer shake flasks and high-efficiency erlenmeyer shake flasks according to their capacity and shape. Their characteristics are as follows: Triangular erlenmeyer shake flask: The capacity of this cell shake flask is generally small, ranging from 125ml-1000ml. It is also called a conical shake flask. It is made of polycarbonate (PC) or PETG. It has a unique triangular shape design. The pipette or cell scraper can reach the corner of the bottle more easily, making the cell culture operation more convenient. It is mainly used for the cultivation of insect cell lines with higher oxygen requirements. Compared with consumables such as cell factories and cell spinner flasks, the cell culture area is smaller, and it is an economical cell culture tool. High-efficiency erlenmeyer shake flasks: This type of erlenmeyer shake flask has a large capacity and is suitable for high-densit...

  E rlenmeyer shake  f lasks  are widely used in the fields of microbiology and cell biology. They can be used with large-capacity culture shakers and are suitable for full-time suspension culture, medium preparation or storage. Cells have very high requirements for the environment, so what material is generally used for  e rlenmeyer  s hake  f lasks ? The material of the  erlenmeyer shake flask  is mainly plastic, mostly polycarbonate (PC) material and PETG material. Polycarbonate (PC for short in English) is a high molecular weight polymer containing carbonate groups in the molecular chain. It is colorless and transparent, heat-resistant, impact-resistant, flame-retardant, BI-level, and has good mechanical properties at ordinary operating temperatures. It has a wide range of applications in the building materials industry, automobile manufacturing, medical equipment, aerospace, packaging, optical lenses and other fields. It can be used for medic...

  Cell culture is also called cell cloning technology, which is an important technical means for biological research. The erlenmeyer shake flasks is a special consumable used in the cell culture process. Understanding the characteristics of the   erlenmeyer shake flasks   and the precautions for use is the prerequisite for the work of cell culture. erlenmeyer shake flasks are generally made of BPA-free PC material or PETG material through injection stretch-blowing technology, and have the following characteristics: 1. The air-permeable membrane area of ​​8L and 5L erlenmeyer shake flasks caps is larger than that of similar products, which is suitable for high-density culture of cells. The working volume can be filled to 60-80% of the total volume, and the cell yield of the same volume of shake flasks is higher. 2. The arc design of the bottleneck of the 2.8 L erlenmeyer shake flask is excessively natural. The size of the bottleneck ensures an effective gas exchange space ...

  The  erlenmeyer shake flasks  is a commonly used cell consumable in the cell culture process. It is mainly suitable for full-time suspension cell culture, medium preparation or storage, and can be used with a large-capacity culture shaker. Cell culture requires high environmental requirements, such as sterility, suitable temperature, pH, and certain nutritional conditions. These conditions need to be achieved with the help of  erlenmeyer shake flasks . Therefore,  erlenmeyer shake flasks  have strict requirements from material to manufacturing process.  erlenmeyer shake flasks  generally use bisphenol A-free PC materials and PETG materials, and the processing adopts a one-step injection stretch-blow molding process, which is also called a one-step injection stretch-blow production process. Its working principle is to first inject the molded blank by an injection molding machine, stretch the hot parison longitudinally, and then pass in compressed...