LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

As a convenient cell culture container,   cell culture flasks   appear frequently in laboratories and are mainly used to cultivate adherent cells. When culturing cells, we need to observe the state of the cells and judge their growth. So, how should we observe? First of all, the state of the cells can be observed through eyes or a microscope. When we take out the cell culture flasks from the incubator, we can judge the growth of the cells by the color, concentration, and cell state of the medium. T25 Flask 1. Look at the color of the medium. There are acid-base indicators in most media, which are yellow when acidic and purple when alkaline. If the pH value of the medium changes greatly, consider whether the medium is contaminated, or the cells grow too densely, or the cells die too much, or there is a problem with the adjustment of the CO2 concentration in the incubator. 2. Look at the concentration of the medium. When the medium becomes turbid, the medium is contaminated or t...

  When conducting cell culture experiments,   cell culture flasks   are a tool that we often use. Its specifications range from 25cm² to 225cm², which can meet the needs of different scales of cell culture. As a common tool, this consumable has the following three characteristics: 1. Torticollar design: The neck of the cell culture flask adopts a torticollar design, which makes it easier for pipettes and cell scrapers to enter the interior, and there is no dead angle in contact, which is convenient for researchers to operate. 2. Two kinds of covers: According to the different culture environment, this consumable has two kinds of covers, airtight cover and air-permeable cover. Caps are often used for closed cultures, but can also be used for open cultures when the caps are unscrewed. The breathable cover is suitable for open culture. The cover is equipped with a 0.2um hydrophobic filter membrane to avoid possible cross-infection during gas exchange. It is generally recomme...

  Cell culture flasks   are mostly used for the cultivation of adherent cells. Different from the growth of suspension cells in the culture medium, adherent cells need to grow on the surface of the container, so the cells must be digested before harvesting or passage. There are many ways to digest cells, the common ones are as follows: 1. Enzyme digestion This is also a method that we use more in our daily life. Generally, trypsin is used for digestion, and the concentration is between 0.25% and 0.5%. The digestion time varies according to the cell type, operation method, temperature and other factors. After digestion, it is necessary to stop the digestion with serum or serum-containing medium. T25 Flask 2. Physical method Without the aid of trypsin, directly pipette or use a cell scraper to digest the cells on the surface of the cell culture flask. This method can be used for cells that are firmly attached. However, it should be noted that pipetting and scraping will cause ce...

  There are thousands of cell types, but they can be roughly divided into two categories: adherent cells and suspension cells. Different cells have different requirements for culture containers. As a consumable product with high frequency of use,   cell culture flasks   are divided into two types: TC-treated surface and non-TC-treated surface. So, what is the difference between these two surfaces? Cells are divided into adherent cells and suspension cells according to different culture methods. The growth of adherent cells must have a support surface that can be attached, and the cells can grow on the surface by relying on the attachment factors secreted by themselves or provided in the medium and reproduce. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes. T225 Flask The cell culture flask is made of polystyrene raw material. Compared with glass material, the surface of this material is hydrophobic, which is ...

  Cell culture flasks   are mainly used for adherent cell culture, and cell culture scale-up in laboratory or industrial production processes. According to the different culture conditions, this cell culture container has two types of lids: Sealed Cap and Vent Cap. So, what is the difference between these two types of caps?            FuDau T25 Cell Culture Flasks The sealing cap is completely sealed and is suitable for cell and tissue culture under sealed conditions, so that the culture environment is completely isolated from the outside world. The breathable cover has a hydrophobic filter membrane with a pore size of 0.22um, which can meet the needs of gas exchange in cell and tissue culture, and can effectively prevent cross-contamination. It is suitable for open culture conditions. The two types of caps for cell culture flasks are first used in different environments. The vent cap is suitable for use in an environment with carbon dioxide, whil...

  There are thousands of cell types, but they can be roughly divided into two categories: adherent cells and suspension cells. Different cells have different requirements for culture containers. As a consumable product with high frequency of use,   cell culture flasks   are divided into two types: TC-treated surface and non-TC-treated surface. So, what is the difference between these two surfaces? Cells are divided into adherent cells and suspension cells according to different culture methods. The growth of adherent cells must have a support surface that can be attached, and the cells can grow on the surface by relying on the attachment factors secreted by themselves or provided in the medium and reproduce. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes. T225 Flask The cell culture flask is made of polystyrene raw material. Compared with glass material, the surface of this material is hydrophobic, which is ...