Hemolysis test of square media bottle

 Square media bottles are mainly used to hold various culture medium, serum, buffer and other solutions required for cell culture. In view of the strict requirements of cell culture tests on consumable products, this kind of packaging needs to go through various tests to ensure that it is qualified before it can be put into use. . Among many testing items, the hemolysis test is a very important one.

Cell hemolysis refers to the phenomenon that the cell membrane bursts outward due to osmotic pressure, quick freezing, mechanical force, biological or chemical toxins, etc., resulting in the outflow of cytoplasmic substances. The hemolysis test in culture media bottles is used to test whether samples contain leachable compounds that would cause hemolysis of red blood cells in vitro.

FuDau 125ml Square PETG/PET Media Bottles

Refer to ISO10993-4:2017: Biological Evaluation of Medical Devices Part 4: Selection of Tests for Interaction with Blood; GB/T 16886.4-2003 Biological Evaluation of Medical Devices - Part 4: Selection of Tests for Interaction with Blood. The negative control and positive control of the test product are in contact with fresh rabbit anticoagulant blood, and the absorbance is measured at a wavelength of 545mm using a UV-visible spectrophotometer, and the hemolysis rate of the test product is calculated, which shall not exceed 5%.

The square media bottle is a common container in cell culture experiments, and the hemolysis test can avoid affecting the later cell culture experiments because the storage container affects the composition of the culture medium.