LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  Cell culture flasks   are mostly used for the cultivation of adherent cells. Different from the growth of suspension cells in the culture medium, adherent cells need to grow on the surface of the container, so the cells must be digested before harvesting or passage. There are many ways to digest cells, the common ones are as follows: 1. Enzyme digestion This is also a method that we use more in our daily life. Generally, trypsin is used for digestion, and the concentration is between 0.25% and 0.5%. The digestion time varies according to the cell type, operation method, temperature and other factors. After digestion, it is necessary to stop the digestion with serum or serum-containing medium. T25 Flask 2. Physical method Without the aid of trypsin, directly pipette or use a cell scraper to digest the cells on the surface of the cell culture flask. This method can be used for cells that are firmly attached. However, it should be noted that pipetting and scraping will cause ce...

  Cell culture flasks   are mainly used for adherent cell culture, and cell culture scale-up in laboratory or industrial production processes. According to the different culture conditions, this cell culture container has two types of lids: Sealed Cap and Vent Cap. So, what is the difference between these two types of caps?            FuDau T25 Cell Culture Flasks The sealing cap is completely sealed and is suitable for cell and tissue culture under sealed conditions, so that the culture environment is completely isolated from the outside world. The breathable cover has a hydrophobic filter membrane with a pore size of 0.22um, which can meet the needs of gas exchange in cell and tissue culture, and can effectively prevent cross-contamination. It is suitable for open culture conditions. The two types of caps for cell culture flasks are first used in different environments. The vent cap is suitable for use in an environment with carbon dioxide, whil...

The previous article introduced four of the common pollution types when using cell culture consumables such as   cell culture flasks   for cell culture. Next, I will introduce the remaining three pollution types and their characteristics: 5. Protozoan pollution: The culture medium may be slightly turbid, and the number of tiny dots under the microscope is very large and slightly mobile. Although the cells can grow, the reproduction speed is obviously slowed down, and the cell state is not good, the edges are not clear, and the cells are not clear. Translucent. They can symbiosis with cells but will compete with cells for nutrients. This symbiosis is very common, but their number is small, and the cell site has an advantage, so it will not affect the normal growth of cells, only when they reach a certain number It will affect the growth of cells and eventually form a vicious circle.      T75 Cell Culture Flasks 6. Virus contamination: In the process of tissue cel...

  Cell culture flasks   are commonly used tools for adherent cell culture, and cell subculture operations are involved in culturing cells. How should we passage adherent cells? You can follow these steps: 1. Take out the preheated culture solution: take out the preheated culture solution, wipe it with an alcohol cotton ball before putting it into the ultra-clean bench. 2. Take out the cells from the incubator: Pay attention to tighten the bottle cap when taking out the cells from the cell culture bottle, wipe the microscope table with alcohol cotton balls, and then observe the cells under the microscope. 3. Put the culture bottle into the ultra-clean workbench and open the mouth of the bottle, and at the same time burn the mouth on the alcohol lamp for disinfection. 4. Add digestive solution: Carefully suck out the old culture solution, wash (rinse) with PBS, add appropriate amount of digestive solution (trypsin solution), pay attention to the amount of digestive solution to c...

  As an economical and affordable cell culture tool, the   erlenmeyer shake flask   has a wide range of applications in the fields of biopharmaceuticals, vaccine preparation, cell and gene therapy, etc. Among them, this kind of container is also used in lentivirus transfection. Lentiviral vectors are gene therapy vectors developed on the basis of HIV-1. Different from general retroviral vectors, it has the ability to infect both dividing and non-dividing cells, and is widely used in the study of expressing RNAi. The cell shake flask is mainly used in the experiment of lentivirus transfection of suspension cells. The steps are as follows:                   125ml-Erlenmeyer-Shake-Flasks 1. Add polybrene to 6 μg/ml and an appropriate amount of virus to 2×105/ml suspended cells, and mix well. Incubate at 37°C. Or centrifuge at room temperature at 150g for 4 hours (selected, this step can improve transfection efficiency for some dif...

  When culturing cells,   cell culture flasks   are an economical and practical culture tool. So what material is it made of? Cell culture flasks generally use highly transparent polystyrene as raw materials. In order to ensure the safety of raw materials, they must meet USP Class VI medical grade standards. The USP class of medical materials is grade 6, ranging from USP class I-USP class VI, with USP class VI being the highest grade. According to USP-NF general rules, plastics subjected to in vivo biological response tests will be classified into designated medical plastic grades. The purpose of the test is to determine the biocompatibility of plastic products and whether they are suitable for medical device implants and other systems.  FuDau T225 Cell Culture Flasks A plastic that is rated as U.S. Medical Plastics Class VI means that comprehensive and rigorous testing has been established. U.S. medical grade VI is now the gold standard for various medical-grade raw...

  With the continuous progress of biotechnology, cell culture technology has also been widely used in genetics, immunology, oncology, virology, molecular biology and other fields. The   cell culture flask   is a commonly used container for adherent culture. The growth of cells requires specific conditions, and the appropriate temperature is the basis. Common specifications of cell culture flasks include T25cm2, T75cm2, T175cm2, T225cm2, small-sized bottles are used for smaller-scale cell culture, and larger-sized bottles can be used for slightly larger-scale cell culture experiments. Since adherent cells need to be attached to the surface of the support to grow, and the material of the bottle itself is hydrophobic, it is suitable for the growth of adherent cells after TC treatment.  FuDau T75 Cell Culture Flasks Generally, the suitable temperature for mammalian and avian cells to be cultured in vitro is 37-38°C. Unsuitable ambient temperature will affect the growth o...

  When culturing cells, we often use   cell culture flasks , whose specifications range from 75cm2 to 225cm2, to meet the needs of cell culture of different scales. Depending on how the cells are cultured, this vessel has two different surfaces: TC-treated and non-TC-treated. Cells are divided into adherent cells and suspension cells according to the different culture methods. The growth of adherent cells must have a support surface that can be attached, and cells can grow on this surface by relying on the attachment factors secreted by themselves or provided in the medium. , breeding. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes.   FuDau T75 Cell Culture Flasks The cell culture flask is made of polystyrene raw material. This material itself is hydrophobic, which is not conducive to the better adherence of cells. Therefore, it needs to be treated with TC on the surface to change its performance, so that th...

  Cell culture flask   is a common cell culture consumable, which can be used for both adherent cell culture and suspension cell culture. Among its many applications, tumor cells are a relatively large category. Tumor cells occupy a central position in tissue culture. First, cancer cells are relatively easy to culture cells. Among the currently established cell lines, there are many cancer cell lines. In addition, tumors are the most threatening diseases to human beings. Tumor cell culture is an extremely important means to study the mechanism of carcinogenesis, detection of anticancer drugs, and molecular biology of cancer, and will play an immeasurable role in elucidating and solving cancer. Tumor cell culture through cell culture flasks is an important way for scientific research institutions and pharmaceutical companies to conduct drug research and disease treatment. The key to its success lies in several aspects, such as material sampling, exclusion of fibroblasts, select...

  Cell culture flasks   are a kind of cell consumables that are often used in the process of cell culture. When culturing cells, various contaminations often cause various troubles to the experiment and hinder the experimental process. So how to deal with these pollution? Cell contamination is generally caused by improper aseptic technique, incomplete sterilization of vessel reagents, and passage of hands or instruments over the open vessel mouth. Once the cells are contaminated, most of them are difficult to recover. The following are the common types and treatment methods of cell contamination: 1. Bacterial contamination: Bacteria are black and fine sand-like under an ordinary inverted microscope. Depending on the infected bacteria, they may have different shapes. The culture medium will generally become cloudy and yellow, which has a significant impact on cell growth. Most of the cells die within 24 hours. Corresponding antibiotic treatment, such as tetracycline, gentamicin...

  Subculture refers to a process in which the culture needs to be divided into small parts, re-inoculated into another culture vessel (bottle), and then cultured.   Cell culture flasks   are commonly used consumables when culturing cells. So, how to passage cells in the cell culture flasks? Generally, the cells in the cell culture flask are basically saturated after they grow into a dense monolayer. In order to enable the cells to continue to reproduce and expand the number of cells at the same time, a passaging operation is required. Suspension cells can be directly divided into flasks, while adherent cells need to be digested before they can be divided into flasks. Adherent cells are generally digested into single cells using proteolytic enzymes (such as trypsin), and ethylenediaminetetraacetic acid (EDTA) can also be added to improve digestion. Observe the cells until the cells become round and detach from the flask wall. This process usually takes 5-15 minutes. To avo...

  Cell culture flasks   are a kind of consumables that are widely used in cell culture experiments. They can be used not only for cell recovery and small-scale cell expansion, but also for the expansion of adherent cells. With the continuous improvement and improvement of processing technology, these consumables are also constantly innovating to better meet the needs of researchers. 1. From the perspective of culture area, cell culture square flasks are available in 25cm2, 75cm2, 175cm2, 225cm2 and other specifications, which can meet the needs of various small and medium-scale cell culture. 2. Bottleneck design: The bottle neck of this consumable adopts a torticollis design, which is convenient for the import and export of culture medium. The neck is wide and has no dead space for easy access to the growth surface for pipettes and cell scrapers. 3. Two types of bottle caps: two types of breathable caps and sealed caps, which can be used in different culture environments. Ther...

  Cell culture flasks   are a kind of cell consumables that are often used in the process of cell culture. When culturing cells, various contaminations often cause various troubles to the experiment and hinder the experimental process. So how to deal with these pollution? Cell contamination is generally caused by improper aseptic technique, incomplete sterilization of vessel reagents, and passage of hands or instruments over the open vessel mouth. Once the cells are contaminated, most of them are difficult to recover. The following are the common types and treatment methods of cell contamination: 1. Bacterial contamination: Bacteria are black and fine sand-like under an ordinary inverted microscope. Depending on the infected bacteria, they may have different shapes. The culture medium will generally become cloudy and yellow, which has a significant impact on cell growth. Most of the cells die within 24 hours. Corresponding antibiotic treatment, such as tetracycline, gentamicin...

  There are many problems when using   cell culture flasks   to culture cells, such as cell non-adherence, cell contamination, cell death, etc. Among them, precipitation in cell culture flasks is also a common situation, which may be related to metal supplements. Metal ions such as copper, iron, and zinc are required for cell growth and are important supplements to serum-free media. The absence of other serum components in the culture system may lead to the precipitation of these metals, creating a toxic environment for the cells. At higher pH (generally >8), carbonate and hydroxide ions and copper ions, phosphate and hydroxide ions and iron ions, carbonate, phosphate and hydroxide ions and zinc ions , hydroxide ions and magnesium ions are easy to form insoluble precipitates; under oxidative conditions, copper and zinc are more likely to precipitate, which is the precipitate we see in cell culture flasks. So how do we avoid the precipitation caused by metal supplements...

  When culturing cells,   cell culture flasks   are a kind of consumable with high usage rate. When culturing cells, they will experience four different growth phases, namely stopper, logarithmic phase, stationary phase and decline phase. These four phases reflect the different growth states of cells. Arrest phase: The phase in which cells adapt to the culture conditions in the cell culture flask, during which the cells do not divide. Cells typically attach within 24 hours of starting the culture, and the total length of this phase depends on the growth phase and seeding density at which the cells were used at the beginning of the culture.    FuDau T25 Cell Culture Flasks Log phase: Cells are actively dividing during this phase and are the optimal time to assess population growth and general data collection. Late in log phase, before cell stress due to overcrowding is the best time to pass cells (subculture). Stationary phase: As cells reach 100% confluence, cel...

  In the process of culturing cells in   cell culture flasks , we encounter various conditions, and slow cell growth is one of them. So, what is the reason for this and how to fix it? There are many reasons for slow cell growth, including the following: 1. Due to the replacement of different culture medium or serum; 2. Some components necessary for cell growth in the culture medium, such as glutamine or growth factors, are depleted or lacking or have been destroyed; 3. There is a small amount of bacterial or fungal contamination in the culture; 4. Improper storage of reagents; 5. The initial concentration of inoculated cells is too low; 6. Cells have aged; 7. Mycoplasma contamination. solution: 1. Compare the composition of the new medium and the original medium, compare the new serum and the old serum to support cell growth experiments, and let the cells gradually adapt to the new medium; 2. Change into freshly prepared culture medium, or add glutamine and growth factors; 3. ...