The importance of shaker speed to cells in cell shaker flasks

 Erlenmeyer shake flask is a kind of cell consumable that is often used in suspension cell culture. Unlike adherent cells, suspension cells grow in suspension in culture medium. In addition to the gas environment, pH, osmotic pressure and other conditions, the speed of the shaker also affects the growth of cells.

Cell culture requires a suitable growth environment and growth temperature, which requires the selection of a suitable shaker for assistance. The constant temperature shaker is a special equipment used in conjunction with the cell shaker. When culturing cells, the shaker needs to be continuously rotated to provide gas conditions for the cells, and at the same time, it can also avoid cell death caused by cell deposition.

          FuDau 125ml Erlenmeyer Shake Flasks 

The specifications of erlenmeyer shake flasks include 125ml, 250ml, 500ml, 1000ml, 3L, 5L, etc. The volume of medium added to the flasks of different specifications is also different, and the oxygen demand of different cells will also be different. Therefore, the rotation speed of the shaker is generally determined according to the type of cells and the capacity of the medium. If the rotation speed is too high, foam is likely to occur, and if the rotation speed is too low, the cells are easily deposited. Both of these conditions are conducive to the growth of cells. Taking BHK-21 cells as an example, the volume ratio of the medium added is generally 25%. The recommended speed of the bottle within 1L is 120 rpm, while the large-capacity shake flask adds more medium, so the speed will be different.

            FuDau 250ml Erlenmeyer Shake Flasks

In summary, the rotation speed of the shaker will directly affect the growth of cells in the erlenmeyer shake flask, and an appropriate rotation speed is the basic condition for improving cell viability. Of course, the specific speed setting also depends on the type of cells and the volume ratio of the medium added.