What is the amount of liquid added to the cell culture erlenmeyer flask?

 In suspension cell culture, cell culture erlenmeyer flask is commonly used as a cell culture consumable. The growth of suspension cells does not rely on the surface of the support and grows in a suspended state in the culture medium. In actual culture, how do we determine the amount of liquid to add?

The common specifications of cell culture erlenmeyer flask include 125ml, 250ml, 500ml, and 1000ml, which meet the needs of different scales of cell culture. For example, 125ml and 250ml small-capacity bottles are mainly used for small-scale experiments, while 500ml and 1000ml specifications are used for medium-scale cell culture experiments. When this consumable is used, the vibration of the shaker is used to reduce the clumping rate of cells and maintain a good growth state of cells. Cell culture needs to be carried out in a sterile environment. Therefore, the triangular culture flask will undergo special sterilization treatment before it is put into use to achieve the effect of no DNase, no RNase, and no animal-derived components, providing a good environment for cell growth. environment.

Cell Culture Erlenmeyer Flask

According to the different bottle specifications, the recommended liquid volume for the four specifications of the bottles from low to high is 30ml, 60ml, 125ml, 500ml respectively. Generally, the volume of the solution when cultivating cells is controlled at about 20%-30% of the total volume of the cell culture erlenmeyer flask, and there are clear scale lines on the bottle body, which is convenient for visual observation of the volume of the solution.

The above are the recommended liquid volumes for cell culture erlenmeyer flask of different specifications. This capacity is not fixed. It should be considered comprehensively according to cell growth characteristics and inoculation density, so as to avoid affecting cell growth due to excessive liquid volume.