LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

As an essential nutrient for cell growth, serum has a good protective effect on cells in culture, and the quality of serum directly affects the growth state of cells.   PETG media bottle   is a special container for storing serum, it must meet some necessary conditions to ensure the stability and safety of serum during storage. Low temperature resistance: The storage of serum is different from ordinary items, and generally needs to be stored in an environment of -5°C to -20°C. If stored at 4°C, try not to exceed one month. This requires that the media bottle must have good low temperature resistance to meet the low temperature storage requirements of serum. PETG Media Bottles Transparency: The quality of the serum, which we can initially discern by its appearance. A good serum should be transparent and clear, earthy yellow or brownish yellow, with no or very little precipitation, and relatively viscous. If the serum is found to be turbid, opaque, and contains many precipitates, it indi

  When conducting cell culture experiments,   cell culture flasks   are a tool that we often use. Its specifications range from 25cm² to 225cm², which can meet the needs of different scales of cell culture. As a common tool, this consumable has the following three characteristics: 1. Torticollar design: The neck of the cell culture flask adopts a torticollar design, which makes it easier for pipettes and cell scrapers to enter the interior, and there is no dead angle in contact, which is convenient for researchers to operate. 2. Two kinds of covers: According to the different culture environment, this consumable has two kinds of covers, airtight cover and air-permeable cover. Caps are often used for closed cultures, but can also be used for open cultures when the caps are unscrewed. The breathable cover is suitable for open culture. The cover is equipped with a 0.2um hydrophobic filter membrane to avoid possible cross-infection during gas exchange. It is generally recommended for culti

  Cell culture flasks   are mostly used for the cultivation of adherent cells. Different from the growth of suspension cells in the culture medium, adherent cells need to grow on the surface of the container, so the cells must be digested before harvesting or passage. There are many ways to digest cells, the common ones are as follows: 1. Enzyme digestion This is also a method that we use more in our daily life. Generally, trypsin is used for digestion, and the concentration is between 0.25% and 0.5%. The digestion time varies according to the cell type, operation method, temperature and other factors. After digestion, it is necessary to stop the digestion with serum or serum-containing medium. T25 Flask 2. Physical method Without the aid of trypsin, directly pipette or use a cell scraper to digest the cells on the surface of the cell culture flask. This method can be used for cells that are firmly attached. However, it should be noted that pipetting and scraping will cause certain dam

  FDA has issued an emergency use authorization (EUA) for the use of Gohibic (vilobelimab) injection for the treatment of COVID-19 in hospitalized adults when initiated within 48 hours of receiving invasive mechanical ventilation or extracorporeal membrane oxygenation (artificial life support). The treatment targets a part of the immune system that is thought to play a role in the inflammation that leads to COVID-19 disease progression. The clinical trial supporting the authorization showed that patients treated with Gohibic had a lower risk of death by day 28 and day 60 of treatment compared to placebo. PETG Media Bottles “COVID-19 treatments remain a priority for CDER, as the disease continues to impact the lives of Americans,” said Center Director Patrizia Cavazzoni, M.D. “Today's authorization offers another potentially life-saving treatment option for the sickest COVID-19 patients.” The recommended dosage of Gohibic is 800 mg administered by intravenous infusion after dilution

  There are thousands of cell types, but they can be roughly divided into two categories: adherent cells and suspension cells. Different cells have different requirements for culture containers. As a consumable product with high frequency of use,   cell culture flasks   are divided into two types: TC-treated surface and non-TC-treated surface. So, what is the difference between these two surfaces? Cells are divided into adherent cells and suspension cells according to different culture methods. The growth of adherent cells must have a support surface that can be attached, and the cells can grow on the surface by relying on the attachment factors secreted by themselves or provided in the medium and reproduce. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes. T225 Flask The cell culture flask is made of polystyrene raw material. Compared with glass material, the surface of this material is hydrophobic, which is not conducive

  Cell culture flasks   are mainly used for adherent cell culture, and cell culture scale-up in laboratory or industrial production processes. According to the different culture conditions, this cell culture container has two types of lids: Sealed Cap and Vent Cap. So, what is the difference between these two types of caps?            FuDau T25 Cell Culture Flasks The sealing cap is completely sealed and is suitable for cell and tissue culture under sealed conditions, so that the culture environment is completely isolated from the outside world. The breathable cover has a hydrophobic filter membrane with a pore size of 0.22um, which can meet the needs of gas exchange in cell and tissue culture, and can effectively prevent cross-contamination. It is suitable for open culture conditions. The two types of caps for cell culture flasks are first used in different environments. The vent cap is suitable for use in an environment with carbon dioxide, while the sealed cap is mainly used in a cu

  There are thousands of cell types, but they can be roughly divided into two categories: adherent cells and suspension cells. Different cells have different requirements for culture containers. As a consumable product with high frequency of use,   cell culture flasks   are divided into two types: TC-treated surface and non-TC-treated surface. So, what is the difference between these two surfaces? Cells are divided into adherent cells and suspension cells according to different culture methods. The growth of adherent cells must have a support surface that can be attached, and the cells can grow on the surface by relying on the attachment factors secreted by themselves or provided in the medium and reproduce. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes. T225 Flask The cell culture flask is made of polystyrene raw material. Compared with glass material, the surface of this material is hydrophobic, which is not conducive

The previous article introduced four of the common pollution types when using cell culture consumables such as   cell culture flasks   for cell culture. Next, I will introduce the remaining three pollution types and their characteristics: 5. Protozoan pollution: The culture medium may be slightly turbid, and the number of tiny dots under the microscope is very large and slightly mobile. Although the cells can grow, the reproduction speed is obviously slowed down, and the cell state is not good, the edges are not clear, and the cells are not clear. Translucent. They can symbiosis with cells but will compete with cells for nutrients. This symbiosis is very common, but their number is small, and the cell site has an advantage, so it will not affect the normal growth of cells, only when they reach a certain number It will affect the growth of cells and eventually form a vicious circle.      T75 Cell Culture Flasks 6. Virus contamination: In the process of tissue cell culture, if the potent

  Serum is a nutrient required for cell culture, and plays an important role in maintaining osmotic pressure, promoting cell attachment and spreading, and maintaining logarithmic growth of cells. The serum is stored in a special   media bottle , but if the quality of the bottle is not up to standard, it will directly affect the quality of the serum. In general, the quality of serum depends on two factors: one is the object of sampling, and the other is the process of sampling. Animals used for sampling should be healthy and disease-free and within the specified number of days of birth. The sampling process should be strictly in accordance with the operating procedures, and the prepared serum should undergo strict quality identification. Requirements in the "Procedures for the Production of Biological Products Using Animal Cells in Vitro Culture" published by WHO: 1. Bovine serum must be from a herd or country that is documented to be free of BSE. and should have an appropriat

  PETG media bottle   is a square plastic bottle with a transparent body and a scale. It is square in shape and is mostly used for the storage of serum or various culture media, buffers, and cell cryopreservation solutions. In addition, it can also be used as a storage container for rabbit red blood cells. Rabbit erythrocytes are aseptically collected anticoagulated rabbit blood, centrifuged to obtain erythrocyte precipitates, washed 2-3 times with an equal amount of PBS until the supernatant is clear, and then prepared to the corresponding ratio of rabbit erythrocytes with Aertenberg’s solution. This kind of cells is mainly used for red blood cell indirect agglutination test, complement fixation test, T lymphocyte E rosette test, production of sensitized cells, immune injection of antibodies, production of blood plate and other tests, and can also be used as an additive for the basis of HB4135 Izumi's medium. 30ml PETG Media Bottles The storage temperature of rabbit erythrocytes i

  Cell culture plays an important role in the pharmaceutical industry, vaccine development and other biomanufacturing fields. Cell growth requires nutrients such as culture medium, and the basic requirement for these nutrients is the absence of cytotoxicity and other factors that affect cell growth. How to detect the cytotoxicity of the   square media bottles   packaged as the media? Cytotoxicity is the action of chemical substances (drugs) on the basic structure and/or physiological processes of cells, such as cell membrane or cytoskeleton structure, metabolic process of cells, synthesis, degradation or release of cell components or products, ion regulation and cell division, etc. , leading to the disturbance of cell survival, proliferation and/or function, and the adverse reactions caused by it. Cytotoxicity detection of square media bottles is mainly based on the change of cell membrane permeability, and the following methods are commonly used: PETG Square Media Bottles 1. MTT and X

PETG media bottle   is a storage container with excellent performance. Thanks to various good properties of the material, it has the characteristics of light weight, non-fragile, high transparency, and low temperature resistance. It is also a commonly used packaging for trypan blue solution. Trypan blue is an organic compound with a chemical formula of C34H24N6Na4O14S4, which is often used as a cell viability dye to detect the integrity of cell membranes and whether cells are alive. Live cells are not stained blue, while dead cells are stained light blue. Trypan blue can be phagocytized by macrophages, so it can be used as a vital stain for macrophages. 60ml PETG Media Bottles Trypan blue solution is mainly for the detection of cell growth and proliferation, it is an important stain that is not absorbed by healthy living cells. When cells are damaged or dead, trypan blue will enter the dead cells, which is convenient for dead cell counting. This method is also sometimes used as a stain

  The square media bottle is a special container for holding the medium. It is generally made of transparent PETG material through injection stretch blowing process. The quality of the bottle is very important for the storage of the medium. So what is the production process of this container? In the production process of the square media bottle, the whole bottle blowing process can be divided into four stages: stretching, pre-blowing, blow molding and cooling. When the temperature adjustment process is completed, the mold is closed, and the stretch rod moves downward to stretch the preform downward along the axis. The lowering speed of the stretching rod is controlled by the stretching cam. The material distribution along the axis of the bottle is formed at this moment. The stretching speed is one of the key parameters affecting the distribution of the material along the axis. If it is too fast, there will be too much material at the bottom of the bottle, resulting in insufficient mate