The difference between the cell culture flask Sealed Cap and the Vent Cap

 Cell culture flasks are mainly used for adherent cell culture, and cell culture scale-up in laboratory or industrial production processes. According to the different culture conditions, this cell culture container has two types of lids: Sealed Cap and Vent Cap. So, what is the difference between these two types of caps?

Cell culture flasks

           FuDau T25 Cell Culture Flasks

The sealing cap is completely sealed and is suitable for cell and tissue culture under sealed conditions, so that the culture environment is completely isolated from the outside world. The breathable cover has a hydrophobic filter membrane with a pore size of 0.22um, which can meet the needs of gas exchange in cell and tissue culture, and can effectively prevent cross-contamination. It is suitable for open culture conditions.

The two types of caps for cell culture flasks are first used in different environments. The vent cap is suitable for use in an environment with carbon dioxide, while the sealed cap is mainly used in a culture environment without carbon dioxide. Secondly, there is no ventilation hole on the surface of the sealing cover, and there is no filter membrane. If ventilation is required, the cover needs to be unscrewed by a quarter of a turn, while the vent cover has a filter membrane, and there are ventilation holes on the top of the cover, which can meet the needs of cell culture. The need for gas exchange.

Cell culture flasks

         FuDau T75 Cell Culture Flasks

On the whole, the difference between the cell culture bottle sealing cap and the vent cap is mainly reflected in the application scenario and whether it has a filter membrane. No matter what kind of cap it is, it is to adapt to different cell culture needs, and it also reflects the different functions of cell culture containers. 


评论

发表评论

此博客中的热门博文

Material features of PETG media bottles

图片
  PETG media bottles   are a special packaging for storing various media, reagents, serum and other solutions, and it is also a product that scientific researchers have more contact with. The wide range of applications is mainly due to the superior properties of its materials. PETG is a transparent plastic that belongs to the non-crystalline copolyester. Serum needs to be stored in a low temperature environment. The use range of this material is -80°C to 60°C, which makes PETG media bottles fully meet the storage needs of serum. In addition, it has the following features: 1. High transparency, the light transmittance reaches 90%, which can reach the transparency of plexiglass; 2. It has stronger rigidity and hardness, excellent scratch resistance, impact resistance and toughness, which is close to or even exceeds that of polycarbonate (PC), and is suitable for injection molding, extrusion molding and extrusion blowing processing; 3. In terms of chemical resistance, oil resistance, weat
阅读全文

What are the common specifications of serum bottles

图片
  In biological laboratories, we often have to store various solutions and reagents. Thanks to the advantages of good performance, transparency, and temperature resistance, serum bottles have become "frequent visitors" in the laboratory. As a commonly used storage container, what are its common specifications?                      FuDau 60ml-Square-PETG-PET-Media-Bottles Common materials for serum bottles include PETG and PET, both of which have very good temperature resistance and transparency. In comparison, bottles made of PETG have more advantages in terms of barrier properties. If you want to pursue a better storage state of the solution, it is recommended to use a bottle made of PETG. There is a molded scale on the body of the bottle, we can clearly observe the capacity and state of the solution. The common specifications of this kind of container include 5ml/10ml/30ml/60ml/125ml/250ml/500ml/1000ml, etc., which are mostly used to store serum, culture medium, buffer, dis
阅读全文

Cell factory requirements for production raw materials

图片
Cell factories   are mainly used for large-scale cell culture. Cells have high requirements on the environment, such as temperature, humidity, osmotic pressure, pH value, etc. Therefore, this kind of   cell   consumables also has high requirements on raw materials. The first is transparency. In the process of cell culture, it is necessary to grasp the growth state of the cells at any time, and make corresponding adjustments according to the cell proliferation. Therefore, the raw materials of the cell factory must meet the requirements of transparency, so that it is convenient for researchers to observe the growth of cells. The second is the compatibility of materials. The raw materials of this consumable generally meet the requirements of USP Class VI medical polymer materials. Medical polymer materials refer to polymer materials used to manufacture human internal organs, external organs, pharmaceutical dosage forms and medical devices, and their sources include natural biopolymer mate
阅读全文