LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

Cell culture is a very delicate work. It is necessary to ensure that the environment is sterile and non-toxic, and the temperature is appropriate, as well as adequate nutrition. Cell consumables are the prerequisite for achieving these ideal conditions, and their selection and use are of vital importance. The  cell factory  is a common consumable for large-scale cell culture work. What are the characteristics of its production materials? The materials of the cell factory are mostly medical-grade polystyrene (English: Polystyrene, referred to as PS), which is mainly used to manufacture medical bottles, syringe trays and packaging materials. Its copolymer with diene and acrylonitrile is commonly known as l1#iS resin. It has only excellent mechanical strength and processing performance. It can be used to prepare adsorption-type artificial kidney shells. It can be made into porous carbonized adsorption resin to remove toxic substances in the patient's blood, such as excessive drugs and

  High-efficiency erlenmeyer shake flasks   are common consumables in cell culture. They are mainly used for the cultivation of mammalian cells, insect cells, yeast cells, fungal cells and other suspension cells. Polycarbonate (PC) is a commonly used raw material for processing. High-efficiency erlenmeyer shake flasks need to observe the growth status of cells in real time when culturing cells, so the transparency of PC material is relatively high. In addition, this material has the following characteristics: 1. Mechanical properties: high strength, fatigue resistance, dimensional stability, and small creep (very little change under high temperature conditions); 2. Heat aging resistance: the enhanced UL temperature index reaches 120~140℃ (long-term outdoor aging resistance is also very good); 3. Solvent resistance: no stress cracking; 4. Stability to water: it is easy to decompose in water at high temperature (use caution in high temperature and high humidity environment); 5. Insulatio

  Erlenmeyer shake flasks   are an important cell culture consumable. They are divided into triangular erlenmeyer shake flasks and high-efficiency erlenmeyer shake flasks according to their capacity and shape. Their characteristics are as follows: Triangular erlenmeyer shake flask: The capacity of this cell shake flask is generally small, ranging from 125ml-1000ml. It is also called a conical shake flask. It is made of polycarbonate (PC) or PETG. It has a unique triangular shape design. The pipette or cell scraper can reach the corner of the bottle more easily, making the cell culture operation more convenient. It is mainly used for the cultivation of insect cell lines with higher oxygen requirements. Compared with consumables such as cell factories and cell spinner flasks, the cell culture area is smaller, and it is an economical cell culture tool. High-efficiency erlenmeyer shake flasks: This type of erlenmeyer shake flask has a large capacity and is suitable for high-density cell cu

  E rlenmeyer shake  f lasks  are widely used in the fields of microbiology and cell biology. They can be used with large-capacity culture shakers and are suitable for full-time suspension culture, medium preparation or storage. Cells have very high requirements for the environment, so what material is generally used for  e rlenmeyer  s hake  f lasks ? The material of the  erlenmeyer shake flask  is mainly plastic, mostly polycarbonate (PC) material and PETG material. Polycarbonate (PC for short in English) is a high molecular weight polymer containing carbonate groups in the molecular chain. It is colorless and transparent, heat-resistant, impact-resistant, flame-retardant, BI-level, and has good mechanical properties at ordinary operating temperatures. It has a wide range of applications in the building materials industry, automobile manufacturing, medical equipment, aerospace, packaging, optical lenses and other fields. It can be used for medical cups, tubes, bottles, dental equipme

  Cell culture is also called cell cloning technology, which is an important technical means for biological research. The erlenmeyer shake flasks is a special consumable used in the cell culture process. Understanding the characteristics of the   erlenmeyer shake flasks   and the precautions for use is the prerequisite for the work of cell culture. erlenmeyer shake flasks are generally made of BPA-free PC material or PETG material through injection stretch-blowing technology, and have the following characteristics: 1. The air-permeable membrane area of ​​8L and 5L erlenmeyer shake flasks caps is larger than that of similar products, which is suitable for high-density culture of cells. The working volume can be filled to 60-80% of the total volume, and the cell yield of the same volume of shake flasks is higher. 2. The arc design of the bottleneck of the 2.8 L erlenmeyer shake flask is excessively natural. The size of the bottleneck ensures an effective gas exchange space and is also co

  The  erlenmeyer shake flasks  is a commonly used cell consumable in the cell culture process. It is mainly suitable for full-time suspension cell culture, medium preparation or storage, and can be used with a large-capacity culture shaker. Cell culture requires high environmental requirements, such as sterility, suitable temperature, pH, and certain nutritional conditions. These conditions need to be achieved with the help of  erlenmeyer shake flasks . Therefore,  erlenmeyer shake flasks  have strict requirements from material to manufacturing process.  erlenmeyer shake flasks  generally use bisphenol A-free PC materials and PETG materials, and the processing adopts a one-step injection stretch-blow molding process, which is also called a one-step injection stretch-blow production process. Its working principle is to first inject the molded blank by an injection molding machine, stretch the hot parison longitudinally, and then pass in compressed air to stretch it laterally to obtain

  Cell culture is also called cell cloning technology, which refers to a method that simulates the internal environment (sterile, suitable temperature, pH, and certain nutritional conditions, etc.) in vitro to make it survive, grow, reproduce and maintain its main structure and function. To achieve the conditions required for cell growth, cell consumables are needed.   Cell culture flasks   are a commonly used type. Cell culture flasks have the characteristics of rich types and optional caps. According to different shapes, it can be divided into square bottles, rotating bottles, triangles, torticollis, etc. Each bottle has its own characteristics and corresponds to different cell culture needs. From the perspective of cell adhesion requirements for products, it is divided into three types: ordinary type, standard type and special type. For example, the special type contains nitrogen-containing functional groups on the surface, which can promote the adhesion and growth of certain specia

  The   cell culture flask   is a culture vessel for cell culture and cell expansion in the laboratory. Cell growth requires a specific environment and temperature. Therefore, when using cell culture flasks for cell culture, you should avoid improper operation and pollution that affect cell growth. 1. Wash your hands before the experiment: Many experimenters do not wash their hands by themselves, and go to the experiment as soon as the alcohol is sprayed. This is a wrong behavior. You should wash your hands carefully with soap/soap, and wash your wrists and nails. It is a good choice to wash your hands twice, and then spray alcohol on your hands. 2. Someone wears a white coat with half sleeves to do experiments. The cuffs of the white coat should be long, which is the kind with the cuffs tied tightly. If not, put the cuffs toward the arm. The bare wrists must also be sprayed with alcohol. Please avoid touching the outside of the workbench after wearing gloves. If you have to touch it,

  There are many types of   cell culture flasks . According to different cell culture needs, there are many types of caps. So how should the caps of cell culture flasks be selected? The caps of cell culture flasks are generally closed without inner pads. They are often used for closed culture to ensure their airtightness. When the cap is unscrewed, it can also be used for open culture. In addition, the caps of culture flasks also have vent caps, septum caps, polyester caps and other types. The breathable cap is a 0.2μm hydrophobic filter membrane added to the bottle cap to provide sterile gas exchange and reduce the risk of contamination. Commonly used for open culture, recommended for co2 incubator culture, especially for experiments that require long-term culture. The septum cover has a pre-cut opening on the septum, so that the suction nozzle (or a pipette of 5ml or less) can be inserted through the septum for adding liquid, aspirating liquid or harvesting cells, reducing the chance

  Cell culture flasks   are a type of cell culture consumables commonly used in adherent cell culture. Adherent cells are different from suspension cells and need to be attached to the surface of a support to grow. The effect of adherence directly affects the growth of cells, and the adherence effect of cells can be improved by the following methods: 1. Appropriate digestion of cells: HepG-2, A549, Hela, SGC-7901 cancer cells can be treated for a longer period of time. Generally, the treatment time is 5-6 minutes. C2C12, COS-7, NIH-3T3 are easier to fall off, and 2 minutes is sufficient. , P19Cl6 and 293 cells do not adhere tightly, so they can be replaced with fresh medium directly after rinsing in PBS to disperse them. 2. Use plastic cell culture bottles as much as possible. After such bottles are treated with TC, the adhesion performance of the bottles can be greatly improved, and the cells can be better attached to them. 3. Resuscitate new cells and use 20% serum to help cells reco

  Cell lysis is an important experimental method to change cell permeability and activity. It means that after a cell is infected by a virus, the virus uses the nutrients in the host cell to synthesize its own offspring, and then the virus offspring destroys the host cell and is released Out of cells. In cell lysis experiments,   cell culture flasks   are a common consumable. The steps for cell lysis are as follows: 1. Pour out the culture medium, and buckle the cell culture bottle upside down on absorbent paper so that the absorbent paper will absorb the culture medium (or put the bottle upright for a while to allow the residual culture medium to flow to the bottom of the bottle and then use a pipette to suck it away ). 2. Add 3ml of 4℃ pre-cooled PBS (0.01M pH7.2 ～ 7.3) to each bottle of cells. Lay the cells flat and gently shake for 1 min to wash the cells, and then discard the washing solution. Repeat the above operation twice, washing the cells three times to wash away the culture

  The   cell culture flask   is a kind of cell consumable that is used in life research and practice. It is mostly used for the cultivation of adherent cells. During use, the correct operation method is the prerequisite to ensure the smooth progress of the experiment. Pay attention to the following when using this consumable What time is it: 1. About the lid: Do not tighten the lid of the disposable cell culture flask too tightly to ensure gas exchange. HEPES is a non-ionic buffer with good buffering capacity in the pH range of 7.2-7.4, but it is very expensive and may be toxic to some cells at high concentrations. HEPES buffer can be used with low-level sodium carbonate (0.34g/L) to offset the increase in osmotic pressure caused by the addition of HEPES. Under this culture condition, the lid of the disposable cell culture flask should be tightened to prevent the small amount of carbonate in the culture solution from dispersing into the air. Most culture fluids contain phenol red as a

  Cell culture flasks   have straight neck, torticollis, angle neck, triangle, rectangle, etc. according to different shapes. The specifications include 25ml, 75ml, 175ml, and 250ml. Smaller sizes are mostly used for cell passaging, preservation of cells, and provision of cells for experiments, etc. use. After the cells grow into a dense monolayer in the cell culture flask, they are basically saturated. In order to enable the cells to continue to grow and increase the number of cells, they must be subcultured (re-cultured). Subculture is also a way to preserve cell species. At the same time, it is a necessary process for various experiments using cultured cells. Suspended cells can be separated directly, while adherent cells need to be digested before they can be separated. The specific steps are as follows: 1. Discard the original culture medium in the cell culture flask full of cells. 2. Add 0.5-1ml 0.25% pancreatin solution, so that the cells at the bottom of the bottle are immersed

  As one of the commonly used consumables for cell culture,   cell culture flasks   play a vital role in the growth and reproduction of cells. At present, the cell culture bottles on the market are mainly made of glass or plastic materials. This kind of consumables is different from ordinary medical plastic bottles, and the price is relatively high. Can this kind of bottles be reused? If you use a glass cell culture flask, you can soak in clean water for more than 30 minutes, add a little cleaning agent, wash with ultrasonic for more than 30 minutes, remove and dry, and then soak in chromic acid lotion for 6-18 hours, then rinse with tap water 10-15 times, rinse with double distilled water 3-4 times, dry, and autoclave before using again. Most of the current cell culture flasks are made of plastic materials, and most of them are disposable and can pass several generations of cells at most. But cells are more sensitive to the environment. If they are reused too many times, the substance

  Cell culture flasks   are a kind of cell consumables often used in the process of cell culture. When culturing cells, various pollutions often cause various problems for the experiment and hinder the process of the experiment. So how to deal with these pollution? Cell contamination is generally caused by improper aseptic technique, incomplete sterilization of utensils and reagents, and passing of hands or instruments over the opening of the utensil. Once the cells are contaminated, most of them are difficult to recover. The following are the common types and treatment methods of cell contamination: 1. Bacterial contamination: The bacteria are black fine sand-like under an ordinary inverted microscope. Depending on the infected bacteria, they can have different shapes. The culture medium will generally become turbid and yellow, which has a significant impact on cell growth. Most of the cells die within 24 hours. Corresponding antibiotics can be added to the culture medium, such as tet

  The   cell culture flask   is a consumable suitable for medium-scale cell tissue culture in the laboratory. The quality of the consumable is related to the process of the experiment and is crucial to the growth and reproduction of the cells. The following factors should be considered when choosing this consumable: 1. Width and shape of the bottleneck: When culturing cells, it involves pipetting and harvesting cells. The design of the wide bottleneck allows the spatula or pipette to enter the bottle more conveniently and facilitates the operation. There is also the use of various enzymes to decompose the cells and then harvest the cells. The curved neck design does not leave a dead corner, which is convenient for the export of the culture medium. 2. Sterilization assurance level: cell growth has strict requirements on the environment, so cell culture flasks must reach a certain level of sterility so as not to affect cell growth. Generally, SAL10-6 is required in the industry, and the

  The   cell culture flasks   is a common cell culture consumable, which can be used for the culture of adherent cells and the culture of suspension cells. Among its many applications, tumor cells account for a relatively large category. Tumor cells occupy a central position in tissue culture. First of all, cancer cells are relatively easy to culture. There are many cancer cell lines in the currently established cell lines. In addition, tumors are the most threatening disease to humans. Tumor cell culture is an extremely important method for studying the mechanism of cancer, anticancer drug detection, and cancer molecular biology. It will play an inestimable role in elucidating and solving cancer. Tumor cell culture through cell culture flasks is an important way for scientific research institutions and pharmaceutical companies to conduct drug research and disease treatment. The key to its success lies in the selection of materials, the elimination of fibroblasts, and the selection of