Five factors to consider when choosing a cell culture flask

 The cell culture flask is a consumable suitable for medium-scale cell tissue culture in the laboratory. The quality of the consumable is related to the process of the experiment and is crucial to the growth and reproduction of the cells. The following factors should be considered when choosing this consumable:

1. Width and shape of the bottleneck: When culturing cells, it involves pipetting and harvesting cells. The design of the wide bottleneck allows the spatula or pipette to enter the bottle more conveniently and facilitates the operation. There is also the use of various enzymes to decompose the cells and then harvest the cells. The curved neck design does not leave a dead corner, which is convenient for the export of the culture medium.

2. Sterilization assurance level: cell growth has strict requirements on the environment, so cell culture flasks must reach a certain level of sterility so as not to affect cell growth. Generally, SAL10-6 is required in the industry, and the non-sterilization rate does not exceed 100%. One in 10,000.

3. Airflow under the culture bottle: Cell culture needs to be stable and stable. There are usually ventilating spokes at the bottom of the bottle to ensure air circulation and maintain temperature balance.

4. Durable design: This mainly refers to the processing materials and wall thickness of the bottle. Good materials and wall thickness can provide better mechanical resistance.

5. Demonstration TC treatment: This consumable is mainly used for the cultivation of adherent cells, and has high requirements on the surface adsorption performance. Generally, it will undergo special demonstrative modification treatment to ensure that the cells can be well attached to the surface of the bottle.

The above are the five factors that need to be considered when choosing a cell culture flask. High-quality consumables are the prerequisite for a successful experiment. When choosing consumables, you should not only consider the price, but also pay attention to the quality of the product.