How to choose the cap of the cell culture flask

 There are many types of cell culture flasks. According to different cell culture needs, there are many types of caps. So how should the caps of cell culture flasks be selected?

The caps of cell culture flasks are generally closed without inner pads. They are often used for closed culture to ensure their airtightness. When the cap is unscrewed, it can also be used for open culture. In addition, the caps of culture flasks also have vent caps, septum caps, polyester caps and other types.

The breathable cap is a 0.2μm hydrophobic filter membrane added to the bottle cap to provide sterile gas exchange and reduce the risk of contamination. Commonly used for open culture, recommended for co2 incubator culture, especially for experiments that require long-term culture.

The septum cover has a pre-cut opening on the septum, so that the suction nozzle (or a pipette of 5ml or less) can be inserted through the septum for adding liquid, aspirating liquid or harvesting cells, reducing the chance of contamination and keeping the culture bottle closed Sterile environment.

Polyester caps are often used for open culture (the bottle cap needs to be loosened), and the gas in the bottle can be exchanged with the gas in the environment by gently loosening the cap.

The different cap types of cell culture flasks correspond to different cell culture methods and environmental requirements. Therefore, you should pay attention to this when purchasing cell culture flasks and choose the appropriate cap according to the needs of cell culture.