博文

目前显示的是 九月, 2023的博文

How to use PC Carboys

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  To use a   PC (polycarbonate) carboy , follow these general steps: 1.Cleaning and Preparing: Before use, ensure that the PC carboy is clean and free from any contaminants. Wash it thoroughly with warm water and a mild detergent, and rinse it well to remove any residue. If required, sterilize the carboy by autoclaving or by using an appropriate sterilizing agent. 2.Filling the Carboy: Carefully pour or transfer the liquid or substance you want to store into the carboy. Ensure that you do not exceed the maximum fill line indicated on the carboy, as overfilling can cause spillage and potential safety hazards. If working with chemicals or hazardous materials, follow the appropriate safety protocols, such as wearing appropriate protective gear (gloves, goggles, lab coat, etc.) and working in a well-ventilated area. 3.Secure the Cap or Closure: Place the appropriate cap, closure, or stopper on the carboy. PC carboys generally come with screw caps or closures that provide a tight seal to pr

The uses of PC Carboys

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  PC (polycarbonate) carboys   have a wide range of uses in various industries and laboratory settings. Here are some common applications for PC carboys: 1.Chemical Storage: PC carboys are frequently used to store and transport various chemicals, including acids, bases, solvents, and reagents. Their excellent chemical resistance allows for safe storage without the risk of leakage or contamination. 2.Biotechnology and Pharmaceutical Production: PC carboys are widely utilized in biotechnology and pharmaceutical industries for the storage and transportation of large volumes of culture media, buffers, solutions, and process intermediates. They can withstand sterilization methods such as autoclaving, ensuring aseptic conditions. 3.Laboratory Research and Development: PC carboys are commonly employed in laboratories for general storage of liquids, such as water, buffer solutions, media, and various reagents. Their transparency allows for easy visual monitoring of the contents. 10L PC Carboys

Two commonly used materials for carboys

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  In biological experiments, the processing and preservation of some liquids are often involved.   Carboys   are ideal for storing sterile liquids, distributing solutions and culture media. The commonly used specifications are mainly 10L and 20L. The material of this container are two main types: PP (polypropylene) and PC (polycarbonate). Polypropylene: It is a polymer formed by the addition polymerization reaction of propylene. The operating temperature range is -30~140℃. It can resist the corrosion of acids, alkalis, salt liquids and various organic solvents below 80℃, and can decompose under high temperature and oxidation. Polypropylene is a thermoplastic synthetic resin with excellent properties. It is a colorless, translucent thermoplastic lightweight general-purpose plastic with chemical resistance, heat resistance, electrical insulation, high-strength mechanical properties and good high-wear resistance processing properties.   Polycarbonate: The liquid storage tank made of this

The characteristics of the carboys

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  In biopharmaceutical or various cell culture experiments, the transfer and storage of liquids are very critical steps, and   carboys   play an important role in these environments. This container has the following characteristics: 1. The barrel body is produced by injection stretch blow molding process, which has better appearance and overall airtightness. 2. The main body of the liquid storage tank is made of PC material, which can withstand high temperature treatment and low temperature storage, and can withstand the temperature range of -80°C~121°C. 3. The capacity of this kind of container is relatively large, the common ones are 10L, 20L, etc. For easy handling, there is a handle design on the barrel body. 10L PC Carboys 5. The capacity of the liquid is a point of concern during the experiment. There are wooden scales on the body of the barrel to facilitate the distribution and use of liquid. 6. The narrow mouth design, the barrel body has high transparency, which is convenient

Advantages of PC Carboys

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  PC (polycarbonate) carboys   are widely used in laboratory and industrial settings for storing and transporting large volumes of liquid. Here are some advantages of using PC carboys: 1.Durability: PC carboys are highly durable and resistant to breakage, making them ideal for withstanding the rigors of handling, transportation, and storage. They are less prone to cracking or shattering compared to glass or other plastic carboys, reducing the risk of accidental spills and contamination. 2.Chemical Resistance: PC is known for its excellent chemical resistance. PC carboys can safely store a wide range of chemicals, including acids, bases, solvents, and corrosive substances. This makes them suitable for various applications in laboratories, pharmaceutical production, and industrial settings where chemical compatibility is crucial. 3.Transparency: PC carboys are transparent, allowing for easy visual monitoring of the liquid level, condition, and any turbidity or precipitation within the co

Application of cell culture flasks in culturing Sf9 cells

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  Sf9 cells are Spodoptera frugiperda cells, which are usually used to produce various medicinal proteins and viruses, or for basic research. When culturing sf9 cells, the commonly used cell culture consumables are   cell culture flasks . Sf9 cells are derived from the Insect Pathology Laboratory of the United States Department of Agriculture and are derived from the IPLBSF-21 cell line. The cells are regular with particles and can be cultured in suspension or adherent culture. This cell has a high expression level (especially intracellular proteins), has a faster growth rate, effective protein folding, glycosylation is similar to mammalian cells, and relatively easy enzymatic deglycosylation (for protein structure determination) Favorable), is widely used in the expression of V LPs. Sf-9 cells are semi-adherent cell lines. In the medium containing 10% fetal calf serum, most of the cells usually grow adherently, and a small part is suspended. Grow in a 27°C cell incubator without CO2.

FuDau Cell Culture Consumables

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Why do cell factory systems need surface TC treatment?

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  The purpose of surface TC treatment in   cell factory systems   is to improve conditions for cell attachment and growth and to increase the efficiency of cell processes. Here are some of the main reasons for surface TC treatment: 1. Improve cell attachment: Surface TC treatment can form a layer of coating or matrix on the surface of the matrix of the cell factory, providing a suitable environment for cell attachment. This coating is usually a highly biocompatible material, such as collagen, gelatin or polylactic acid, which can be used for surface treatment of cell culture media. Through surface TC treatment, cell adhesion factors, growth factors and extracellular matrix components required for cell adhesion can be provided, thereby enhancing the interaction between cells and the matrix, and facilitating the attachment and growth of cells in the factory. 2. Promote cell proliferation: Surface TC treatment can provide suitable surface properties required for cell division and prolifer

Cell culture consumables commonly used in laboratories and their uses

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  Cell culture consumables are indispensable experimental tools in the cell culture process. Commonly used consumables include   cell culture flasks , cell culture dishes, and cell culture plates. So what are their respective uses? 1. Cell culture dishes: Cell culture dish is a laboratory vessel used for microorganism or cell culture. It consists of a flat disc-shaped bottom and a cover, usually made of glass or plastic. The materials of culture dishes are basically divided into two categories, mainly plastic and glass. Glass can be used for plant materials, microbial culture and adherent culture of animal cells. The plastic ones may be made of polyethylene, and are disposable or multiple-use. They are suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can be used for the cultivation of plant materials. Cell Culture Dishes 2. Cell culture plates: Cell culture plate can be divided into flat bottoms and round bottoms (U-shaped and V-shaped) according

FDA Approves First Biosimilar to Treat Multiple Sclerosis

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  August 24, 2023, The U.S. Food and Drug Administration today approved Tyruko (natalizumab-sztn), the first biosimilar to Tysabri (natalizumab) injection for the treatment of adults with relapsing forms of multiple sclerosis (MS). Tyruko, like Tysabri, is also indicated for inducing and maintaining clinical response and remission in adult patients with moderately to severely active Crohn’s Disease (CD) with evidence of inflammation who have had an inadequate response to, or are unable to tolerate, conventional CD therapies and inhibitors of TNF-α (tumor necrosis factor, a substance in your body that causes inflammation). “Biosimilar medications offer additional effective treatment options that have the potential to increase access for people living with relapsing forms of multiple sclerosis,” said Paul R. Lee, M.D., Ph.D., director of the Division of Neurology 2 in the FDA’s Center for Drug Evaluation and Research. “Today’s approval could have a meaningful impact for patients managing

Application of Cell Factory Systems in Production of Inactivated Vaccine

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  Cell factory systems   are a common cell culture tool during the preparation of inactivated vaccines. It adopts a multi-layer structure design, which not only takes up less space but has a large cultivation area, and can realize automatic operation with the help of special equipment. Do you know where this container is mainly used in vaccine preparation? Inactivated vaccines are made by culturing viruses on a cell matrix, and then using physical or chemical methods to kill infectious viruses while maintaining the integrity of their antigenic particles, making them lose their pathogenicity but retain their antigenicity.  Inactivated vaccines can be composed of whole viruses or bacteria, or they can be composed of their split fragments as split vaccines. The inactivated vaccines currently used in China include inactivated polio vaccine, Japanese encephalitis inactivated vaccine, influenza vaccine, rabies vaccine, hepatitis A inactivated vaccine, EV71 hand, foot and mouth disease vaccin

FDCELL at Arab Lab 2023

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  Join us at Arab Lab 2023 in Dubai from Sep 19 to 21 at Dubai International Exhibition Center, Booth S1/851! Looking forward to seeing you there! More: https://www.cellcultureflasks.com/