FuDau Cell Culture Consumables

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What characteristics do the culture media bottles need to meet?

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  The media is the basic substance that provides cell nutrition and promotes cell proliferation, and is also the living environment for cell growth and reproduction. There are many types of medium, the raw materials for preparation are different, and the requirements for use are different. What characteristics should the   media bottles   meet to ensure that the medium is well preserved? The general medium is easily contaminated or decomposed by bacteria after being heated and absorbing moisture. Therefore, the general medium must be kept in a cool and moisture-proof place. For some media (such as tissue culture medium) that need to be strictly sterilized, they must be stored in a refrigerator at 3-6 °C for a long time. To ensure good storage of the medium, the media bottle should meet the following characteristics: 1. Sterility: The medium bottle must be aseptically treated to ensure that the medium is not contaminated. The general sterilization method will choose electr...
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What to do if there is Nanobacteria contamination in the cell factory

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  Nanobacteria is often encountered in cell culture. After the cells are contaminated, the adherent growth will not be affected in a short period of time, and the medium will not quickly turn yellow or turbid like bacteria and fungi. So, what to do if there is Nanobacteria  contamination in the  cell factory ? There is no clear conclusion about Nanobacteria, and it is generally believed that the black matter is the debris produced by the deterioration and death of cells due to various reasons. It can penetrate the filter membrane or spread through the air. It is black dots at low magnification, and black bugs can be seen swimming around at high magnification. The culture medium is also not muddy, generally it will not be too affected, and the cells can still be used. Usually, the cells grow well, and the observed movement does not increase significantly, and there is no obvious change in the color and transparency of the culture medium. Similar phenomena can be found in t...
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How to use the cell factory

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  The rapid development of the pharmaceutical industry has allowed cell culture technology to develop its ambitions. With the help of cell culture consumables such as   cell factories , it plays an important role in industrial mass production, such as vaccines, monoclonal antibodies or the pharmaceutical industry. Correct use can make cell culture work more efficiently, so how should cell factories be used? The use of the cell factory should follow certain methods and steps. Improper operation will affect the later cell culture work. The steps for using the cell factory are as follows: 1. Add the required amount of cell suspension to the cell factory by pouring. The recommended volume for each layer is 200ML. 2. After adding the cell suspension, keep the cell factory on its side, with the side with the liquid addition port facing away from you, and balance for a period of time so that the liquid is evenly distributed to each layer. 3. Rotate 90 degrees counterclockwise, so tha...
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