FuDau Cell Culture Consumables

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Analysis of the causes of cell clumping in cell culture flasks

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  Cultivating cells is a very rigorous task. In addition to creating a good growth environment for cells, it must also promptly resolve various emergencies during the culture process. Cell clumping in   cell culture flasks   is a phenomenon often encountered. What is the reason for this situation? The clumping phenomenon of cells in the cell culture flasks can generally be analyzed from the following aspects: 1. The digestion process may be too rough, pipetting too hard, digestion for too long, too strong or toxic digestive juice, etc., will cause cell death. After the cell dies, it releases DNA, entangles other cells, and forms a sticky cell cluster. 2. Excessive cell centrifugation speed or too long time can also easily cause cell clumping. 3. When the digestion is not complete, the connection between the cells and the cell is not separated; when the digestion is over, the round cells tend to pile up and it is difficult to separate again. 4. Cells that are in poor condi...
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Functional innovation of cell culture flasks

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Cell culture flasks   play an important role in medium-scale cell and tissue culture in the laboratory. Common specifications include 25cm2, 75cm2, 175cm2, and 225cm2, which can meet the needs of cell culture of different scales. Different from ordinary plastic bottles, the innovations in the design of cell culture flasks are reflected in the following aspects: 1. Bottleneck: The bottleneck of the cell culture flask is designed with torticollis, and the bottom is in the shape of an arc, which is convenient for the export of the culture solution. The bottleneck is relatively wide and there is no dead corner, so that the pipette and the cell scraper can go deep into every corner of the bottle, and the operation is more convenient. 2. Bottle cap: This cell consumable is equipped with two different bottle caps, which are suitable for different culture environments. The sealing cap is in a completely sealed state, and there are no vents on the cap. It is mainly used under carbon dioxide...
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See the different uses of cell culture flasks from specifications

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  The  cell culture flask   is a special cell culture consumable with a square and wide bottleneck design. According to the different needs of use, this consumable is divided into 25cm2, 75cm2, 175cm2, 225cm2 and other specifications to meet the different needs of experimenters. Small size: The size of the cell refers to the culture area of ​​the cell culture flask. The 25cm2 and 75cm2 bottles are mainly used for cell recovery and small-scale expansion. Cell resuscitation is a biological term that refers to the process of re-cultivating cells frozen in liquid nitrogen or in a refrigerator at -70°C to restore growth. Cell expansion refers to expanding and reculturing existing cells. Large size: 175cm2 and 225cm are mainly suitable for medium-scale cell culture and eukaryotic protein expression. When used for cell culture, it usually refers to adherent cells, and some suspension cells also use this consumable. Eukaryotic protein expression is a commonly used method for expr...
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