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Cause Analysis of Mycoplasma Contamination in Cell Factory

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  Mycoplasma contamination of cells is a very troublesome problem when culturing cells, especially in the use of large-scale culture vessels such as   cell factory . Once mycoplasma contamination occurs, the loss will be even greater. So, what is the cause of mycoplasma contamination? Mycoplasma is a prokaryotic organism with a size of only 0.2-0.3 um, no cell wall, and can pass through a general filter membrane (0.22-0.45 um). During cell culture, the incidence of mycoplasma infection reaches 63%, so during cell culture Contamination by mycoplasma is a worldwide problem. When cells (especially passage cells) are contaminated by mycoplasma, the expression of DNA, RNA and protein in the cells will change, but the growth rate of cells generally does not have a significant impact, so the contamination of cells by mycoplasma is generally difficult to detect. In most cases, the cytopathic changes are slight or insignificant, and subtle changes can also be alleviated by subculture a...
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How to deal with various contaminations in cell culture flasks

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  Cell culture flasks   are a kind of cell consumables that are often used in the process of cell culture. When culturing cells, various contaminations often cause various troubles to the experiment and hinder the experimental process. So how to deal with these pollution? Cell contamination is generally caused by improper aseptic technique, incomplete sterilization of vessel reagents, and passage of hands or instruments over the open vessel mouth. Once the cells are contaminated, most of them are difficult to recover. The following are the common types and treatment methods of cell contamination: 1. Bacterial contamination: Bacteria are black and fine sand-like under an ordinary inverted microscope. Depending on the infected bacteria, they may have different shapes. The culture medium will generally become cloudy and yellow, which has a significant impact on cell growth. Most of the cells die within 24 hours. Corresponding antibiotic treatment, such as tetracycline, gentamicin...
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Cell culture consumables commonly used in laboratories and their uses

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  Cell culture consumables are indispensable experimental tools in the cell culture process. Commonly used consumables include   cell culture flasks , cell culture dishes, and cell culture plates. So what are their respective uses? 1. Cell culture dishes: Cell culture dish is a laboratory vessel used for microorganism or cell culture. It consists of a flat disc-shaped bottom and a cover, usually made of glass or plastic. The materials of culture dishes are basically divided into two categories, mainly plastic and glass. Glass can be used for plant materials, microbial culture and adherent culture of animal cells. The plastic ones may be made of polyethylene, and are disposable or multiple-use. They are suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can be used for the cultivation of plant materials. Cell Culture Dishes 2. Cell culture plates: Cell culture plate can be divided into flat bottoms and round bottoms (U-shaped and V-shaped) a...
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