250ml Square Media Bottles Sufficient inventory, Short Delivery Time, New Price, High Cost Performance! Your Best Choice! If you want to get free samples and any other question, please leave a message or send an email to contact us! 250ml Square Media Bottles are mainly used for storing and sampling active pharmaceutical ingredients, bulk intermediates, and also for preparing, storing buffers, culture.solutions or long-term storage of pH sensitive liquids. It can be used to store reagents but cannot be used as heating vessels. Features Type: 250/500/1000ml. Material:PET/PETG Production Environment:C-GMP Sterilization:60co-γ irradiation SAL=10, Non-Pyrogenic, Dnase & Rnase free. The bottle body is square, easy to hold, easy to store. The surface is smooth and shiny, easy to clean, not easy to leave residue. Good chemical resistence Low temperature resistence: -70 Product Number Specification Textures Neck D IA (mm) High(mm) P...
Cell culture flasks play an important role in medium-scale cell and tissue culture in the laboratory. Common specifications include 25cm2, 75cm2, 175cm2, and 225cm2, which can meet the needs of cell culture of different scales. Different from ordinary plastic bottles, the innovations in the design of cell culture flasks are reflected in the following aspects: 1. Bottleneck: The bottleneck of the cell culture flask is designed with torticollis, and the bottom is in the shape of an arc, which is convenient for the export of the culture solution. The bottleneck is relatively wide and there is no dead corner, so that the pipette and the cell scraper can go deep into every corner of the bottle, and the operation is more convenient. 2. Bottle cap: This cell consumable is equipped with two different bottle caps, which are suitable for different culture environments. The sealing cap is in a completely sealed state, and there are no vents on the cap. It is mainly used under carbon dioxide...
Lysogenic bacteria refer to bacteria that have prophages integrated on their chromosomes and can grow and reproduce normally without being lysed. Mild bacteriophages have been widely used in the fields of transduction, genetic engineering vectors, and molecular biology. The cell culture flask can be used to check the lysogenicity of bacteria. The specific operation method is as follows: 1. Culture of lysogenic bacteria: Inoculate E. coli 225 (λ) activated by the LB slope into a 250ml cell culture flask containing 20ml LB culture solution, culture with shaking at 30°C for 16 hours, and then take 2ml bacterial suspension from it and connect it to another In a 250ml Erlenmeyer flask containing 20ml of LB broth, culture with shaking at 37°C for 2h to logarithmic phase. 2. Exclude free phage: If the strain to be tested is Bacillus, it can be made into a spore suspension, and then treated at 80°C for 10 minutes to kill the phage that may be adsorbed and free on the surfac...
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