LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  Mycoplasma contamination of cells is a very common problem. Mycoplasma contamination is not easy to find. After cells are contaminated by mycoplasma, a symbiotic system will be formed, resulting in the continuous expansion of the contamination, which in turn affects the growth of cells. So, how can we prevent mycoplasma contamination in   cell culture flasks ? FuDau T25 Cell Culture Flasks When cells are contaminated by mycoplasma, the expression of DNA, RNA and protein in cells changes, but the growth rate of cells generally does not have a significant impact. With the further expansion of pollution, the metabolic function of cells will be reduced, which will affect cell culture. Results of the experiment. The causes of mycoplasma contamination in cells in cell culture flasks may be cross-contamination between cells, operator's mouth and skin, working environment or experimental equipment contamination, culture medium contamination, etc. Mycoplasma contamination is difficult to

  Media bottles   are special containers for storing serum, culture medium, trypsin, reagents and other products. These solutions are generally used for cell culture, so the packaging must meet the requirements of no endotoxin, so as not to affect the normal growth of cells. So how do you remove endotoxins from containers? The chemical composition of endotoxin is lipopolysaccharide, which is one of the main components of the cell wall of Gram-negative bacteria. A live bacterium releases very little endotoxin, but a large amount of endotoxin can be released after death. Because endotoxin can stimulate many kinds of cells and affect the experimental results, the source of endotoxin in the culture medium should be minimized in the experiment, and the packaging container is an important aspect. At present, the serum bottles on the market are mainly made of glass or PET/PETG, and endotoxins can be tightly adhered to the glassware. The common methods in the laboratory cannot completely elimi

Fetal bovine serum is obtained from caesarean section fetuses, which is of high quality, because the fetuses have not been exposed to the outside world, and the serum contains less antibodies, complements and other components that are harmful to cells. Factors affecting the quality of fetal bovine serum include appearance, technical indicators, blood source,   media bottle   and other four aspects. Appearance: Including the color, clarity, and precipitation of the serum. Generally, domestic fetal bovine serum is waxy yellow, imported high-quality serum is pale yellow, reddish, and slightly orange-red or bright red. Heat-inactivated serum or improperly stored serum will appear dark red or even brown due to the destruction and oxidation of hemoglobin. Technical indicators: Technical indicators affecting serum quality include endotoxin, total protein content, hemoglobin, PH, microorganisms, immunoglobulins, etc., as well as the determination of antibiotics. Domestic dairy cattle breeding

  Serum is the most widely used type of cell culture medium, especially high-quality fetal bovine serum. With the rapid development of biomedical technology, the demand for serum is also rising, so what is the future market demand for   media bottles   as serum storage containers? Serum, as a nutrient for cell growth, is mainly used in the early cell culture stage of biopharmaceutical industrial production. Biopharma has been at the forefront of the entire pharmaceutical industry, representing both knowledge-intensive and capital-intensive industries. Since the beginning of the new century, breakthroughs have been made in the research of life science and biomedicine, and biotechnology has become the focus of the world's scientific and technological competition. The global biopharmaceutical industry has developed rapidly in recent years, and a large number of new drugs have passed clinical trials. According to relevant statistics, the world's top ten best-selling drugs and biolo

Media bottle is a special packaging made of transparent polyester (PET/PETG) raw material with organic solvent added through injection stretch blow production process. In order to facilitate holding, the shape of the bottle is generally square, and the bottle body is marked with a scale. So, what are the main applications of   media bottles ? 1. Serum storage: Storage of serum is an important application of media bottles. Serum is a complex mixture formed by removing fibrinogen from plasma and should be stored at -5°C to -20°C. If stored at 4°C, it should not exceed one month. If one bottle cannot be used up at a time, it is recommended to aseptically aliquot the serum into an appropriate sterilized container and put it back into the freezer. 2. Medium storage and preparation: Medium is an artificially formulated nutrient for the growth and maintenance of microorganisms, plants and animal tissues, generally containing water, nitrogen sources, inorganic salts (including trace elements),

Cell culture needs to be carried out in a sterile environment, and serum, which is a nutrient for cell growth, must also be aseptically processed before it can be used. In order to ensure the sterility of the serum, qualified sterile   media bottles   must also meet the sterility requirements. The sterile media bottle is generally made of transparent PET material, which has good low temperature resistance and impact resistance, is non-toxic and tasteless, and has high transparency. It is an excellent packaging material. In the sterilization method, this kind of bottle is generally sterilized by electron beam irradiation. Electron beam irradiation is a technique commonly used for the sterilization of pharmaceutical packaging products and medical devices. It works by directing a continuous flow of electrons through the item being sterilized. The transfer of electron beam energy breaks the DNA chain in the organism, allowing the It loses its activity and the irradiated article becomes ste