How to achieve sterility requirements in sterile media bottles

Cell culture needs to be carried out in a sterile environment, and serum, which is a nutrient for cell growth, must also be aseptically processed before it can be used. In order to ensure the sterility of the serum, qualified sterile media bottles must also meet the sterility requirements.

The sterile media bottle is generally made of transparent PET material, which has good low temperature resistance and impact resistance, is non-toxic and tasteless, and has high transparency. It is an excellent packaging material. In the sterilization method, this kind of bottle is generally sterilized by electron beam irradiation. Electron beam irradiation is a technique commonly used for the sterilization of pharmaceutical packaging products and medical devices. It works by directing a continuous flow of electrons through the item being sterilized. The transfer of electron beam energy breaks the DNA chain in the organism, allowing the It loses its activity and the irradiated article becomes sterile.

Electron beam sterilization is convenient, gentle, effective, and can be performed continuously on the final packaged product. It operates at a much lower temperature than steam sterilization and is suitable for products with hard-to-reach areas (eg, cartridges with lids). Compared to gamma radiation, it has a much lower risk of degradation (due to shorter exposure times); compared to ethylene oxide exposure, it eliminates the risk of ethylene oxide absorption and subsequent penetration into the drug product.

The sterile media bottle after electron beam sterilization can reach a sterile state and meet the storage requirements of serum, which is of great significance for the orderly growth of cells.