How to Prevent Mycoplasma Contamination in Cell Culture Flasks

 Mycoplasma contamination of cells is a very common problem. Mycoplasma contamination is not easy to find. After cells are contaminated by mycoplasma, a symbiotic system will be formed, resulting in the continuous expansion of the contamination, which in turn affects the growth of cells. So, how can we prevent mycoplasma contamination in cell culture flasks?

FuDau T25 Cell Culture Flasks

When cells are contaminated by mycoplasma, the expression of DNA, RNA and protein in cells changes, but the growth rate of cells generally does not have a significant impact. With the further expansion of pollution, the metabolic function of cells will be reduced, which will affect cell culture. Results of the experiment. The causes of mycoplasma contamination in cells in cell culture flasks may be cross-contamination between cells, operator's mouth and skin, working environment or experimental equipment contamination, culture medium contamination, etc. Mycoplasma contamination is difficult to find, so prevention is the key.

Start by adhering to good cell culture practices and culturing only one cell line at a time to prevent cross-contamination between cells. Secondly, strictly abide by the laboratory aseptic operation procedures, and do a good job of personal hygiene and environmental sterilization. Third, it is necessary to ensure that the cell culture medium, serum, reagents, cell culture flasks and other consumables used are sterile. Finally, an appropriate amount of antibiotics can also be added to the medium to prevent the occurrence of mycoplasma contamination.

FuDau T75 Cell Culture Flasks

In a word, mycoplasma contamination is a contamination source that is not easy to be found, but its impact on the cells in the cell culture flask is also very serious. Taking various precautions and strictly following aseptic operations can reduce the probability of contamination.