LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

With the rapid development of industries such as biopharmaceuticals, vaccines, and monoclonal antibodies, the importance of   cell factories   in large-scale cell culture has become increasingly prominent. Cell culture is a rigorous work, with strict control over the cell growth environment, the addition of culture media, and the harvest of cells. When using the cell factory to harvest cells, the following procedures must be strictly followed: 1. Pour out the culture solution after incubation, and wash it with calcium-free and magnesium-free phosphate buffer (CMF-PBS) (40-50 ml/layer), and repeat the washing if necessary. 2. Digestion: The digestion solution (10-40 ml/layer) is preheated in advance. 3. Collection: Centrifuge at 1000 rpm for 5 minutes to remove the digestion solution and collect the cells. 4. Washing: Wash the digested incubator with CMF-PBS or culture medium. Pay attention to the following points when harvesting cells: 1. Make sure that CMF-PBS is completely immersed i

The   cell factory   is a well-designed cell culture device that makes full use of the maximum culture surface in a limited space, thereby saving space; it can be used for industrial-scale production such as vaccines, monoclonal antibodies or biopharmaceuticals, and is particularly suitable for Adherent cells can also be used in suspension culture. The specific operation process of the cell factory is as follows: 1. Unscrew the lid, inject the culture medium into the cell factory by tilting or pumping, and the cell suspension will slowly flow into each layer of the cell factory, cover the lid and stay still; 2. Slowly put the cell factory on its side, the side with the liquid filling port facing away from you, and let it stand to make the cell suspension flow slowly and evenly into each layer; 3. Rotate 90 degrees laterally, keep the liquid inlet upward, and let it stand to make the cell suspension flow into each layer slowly and evenly; 4. Hold the side of the liquid inlet with both h

The   cell factory   is a well-designed cell culture device. The cell factory culture technology is a new generation of cell culture technology that uses the cell factory for large-scale cell culture, and has been widely used in the vaccine industry. The cell factory culture technology has been applied in foreign countries for more than 40 years, but it has been popularized in my country a little later. It uses the cell factory to maximize the cultivation surface in a limited space, thereby saving a lot of workshop space. It can achieve the purpose of expanding production capacity without any workshop modification. It is especially suitable for adherent cell culture and can also be cultured according to The area increases the number of layers of the cell factory. Cell factory culture technology has been widely used in the vaccine industry. For example, hepatitis A vaccine, which uses cell factories for cell culture and virus amplification technology has been very mature. In addition, i

  Cell factories   play an important role in large-scale cell culture. At present, the common specifications of cell factories are 1, 2, 5, 10, 40, etc. The culture area corresponding to different layers is also different, so different How many cells can be inoculated in a cell factory of the specifications? Because of the different growth characteristics of each type of cell and the requirements for inoculation density and environment, it is impossible to generalize how many cells can be inoculated with each size of cell factory. Cell seeding density refers to the density of live cells seeded, which affects cell growth and survival to a large extent. When the inoculation concentration is low, the cell growth and reproduction are slower, and the final cell yield is also low, especially for adherent animal cells. When the inoculation concentration is low to a certain critical value, the cells will not even expand on the medium surface. Different cell lines have different appropriate cel

  In recent years, thanks to the rapid development of the biological products industry, cell culture technology has been widely used in the fields of monoclonal antibodies and virus vaccine products. Among many culture methods,   cell factory   culture technology is a common large-scale cell culture method, which has the following advantages: 1. Save manpower, material resources, time and space. The cultivation area of the cell factory can maximize the use of space, thereby saving a lot of workshop space and saving manpower and material resources to a certain extent. It can be used in combination with automated operating equipment to realize automated cell culture and greatly reduce labor intensity and intensity. 2. Convenient to scale up In general culture methods, if you want to expand the scale of cell culture, you will increase the number of cell consumables. While increasing the number, it also takes up more space and manpower. The cell factory culture technology wants to increase

  Cell culture is a special task that consumes energy. It requires not only suitable temperature, osmotic pressure, gas, pH value, culture medium and other conditions, but also regular observation of cell growth. So, for consumables with a multi-layer structure like the   cell factory , how do you observe the growth status of the cells? When the cell factory is cultured, the cell growth status can be observed with an inverted microscope or a cell microscopic observation monitor. The difference is that an inverted microscope can only observe the cell growth on the bottom 1-2 layers, while the cell microscopic observation detector can You can observe the growth of more cells with the help of high-precision cold-light LED lights. Cells in a good growth state can be seen under observation under a normal microscope. The transparency of the cells is large and the outline is not clear. Only by observing with a phase-contrast microscope can the fine structure of the cells be seen clearly. Ther

  With the development of science and technology, more and more cell factories are used in the large-scale cultivation of cells. Compared with traditional roller bottle culture, the   cell factory   has the advantages of large cultivation area, less space, less manual operation, and can be combined with automated equipment. Due to the culture characteristics of cell factories, the easiest way to scale up is to increase the number of cell factories. When the number of cell factories increases, we will encounter the same trouble as when using cell flasks for culturing-multiple opening operations. As the number of lid openings increases, the operation time will be prolonged, and the risk of contamination will also increase. In order to solve this problem, a very simple way can be used to form a group of cell factories from individual units, and to combine open culture components into a closed system. The operation method is as follows: 1. Prepare customized transfer caps, culture media, c

FuDau  Cell Factory is compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications such as production of vaccines, monoclonal antibodies or pharmaceuticals. They’re versatile and easy-to-use system for medium to large scale research or commercial production with reduced contamination risk.Narrow mouth caps available for tubing solutions. FuDau Cell Factory  Features    Growth kinetics are the same as cells grow in the cell culture flasks. Wide mouth is applicable to pour culture medium directly. Narrow mouth is applicable to operate with the aseptic pipeline Non-Pyrogenic, DNase/Rnase free. TC treated, good for cell attachment and growth. Vent caps with 0.22 μm hydrophobic filters for gas exchange without contamination. FAQ: 1. Q: Is there a product batch number identification for traceability? Answer: There is a product batch number label, which can be traced back to each day. 2. Q: What is the material of the hydrophobic membrane of the

  Cell culture is inseparable from various cell consumables. As a production device suitable for large-scale cell culture, cell factories have been increasingly applied to the production and preparation of vaccines and other biological products. The  cell factory  is composed of one or more layers. According to the cell culture scale, the cell factory corresponding to the culture area can be selected. In addition, the cell factory also has the following characteristics: 1. After the cell factory enters the liquid and the bottle mouth adopts a wide-mouth design, the filling and harvesting of the liquid are relatively rapid, and it is not easy to produce bubbles. At the same time, the large-mouth design is more conducive to gas exchange and is suitable for high-density cell culture. 2. The standard cell factory is equipped with a 0.2μm sterile breathable cover and a sealing cover, which can be used in different culture environments. The sterile breathable cover is used in a CO2 environme