How to observe the growth status of cells in the cell factory

 Cell culture is a special task that consumes energy. It requires not only suitable temperature, osmotic pressure, gas, pH value, culture medium and other conditions, but also regular observation of cell growth. So, for consumables with a multi-layer structure like the cell factory, how do you observe the growth status of the cells?

When the cell factory is cultured, the cell growth status can be observed with an inverted microscope or a cell microscopic observation monitor. The difference is that an inverted microscope can only observe the cell growth on the bottom 1-2 layers, while the cell microscopic observation detector can You can observe the growth of more cells with the help of high-precision cold-light LED lights.

Cells in a good growth state can be seen under observation under a normal microscope. The transparency of the cells is large and the outline is not clear. Only by observing with a phase-contrast microscope can the fine structure of the cells be seen clearly. There are few particles in the cells, no vacuoles, clear cell membranes, clear and transparent culture medium, no suspended cells and debris can be seen; when the cell function is poor, the outline is enhanced, and vacuoles, lipid droplets and other particulate matter often appear in the cytoplasm. As the gap between them increases, the cell morphology may become irregular, or even lose its original characteristics.

Observing the growth status of cells is a necessary step for cell factory culture. Only cells in good growth status can be used in experiments. Generally, after cell inoculation or passage, observe the cell morphology, growth, pH of the culture medium and contamination or not every day or at least 1-2 days, and keep abreast of the dynamic changes of the cells for medium replacement or passage treatment. If abnormal conditions are found, take immediate measures.