How many cells can be seeded in the cell factory

 Cell factories play an important role in large-scale cell culture. At present, the common specifications of cell factories are 1, 2, 5, 10, 40, etc. The culture area corresponding to different layers is also different, so different How many cells can be inoculated in a cell factory of the specifications?

Because of the different growth characteristics of each type of cell and the requirements for inoculation density and environment, it is impossible to generalize how many cells can be inoculated with each size of cell factory. Cell seeding density refers to the density of live cells seeded, which affects cell growth and survival to a large extent. When the inoculation concentration is low, the cell growth and reproduction are slower, and the final cell yield is also low, especially for adherent animal cells. When the inoculation concentration is low to a certain critical value, the cells will not even expand on the medium surface. Different cell lines have different appropriate cell seeding densities.

It is generally recommended that researchers can inoculate the number of cells per unit area of the cell factory, multiply it by the used cell factory culture area, and divide by the inoculation ratio of the cultured cells. Too high or too low cell inoculation amount is not conducive to cell reproduction. A proper cell inoculation amount is a prerequisite to ensure the normal growth of cells.

The cell factory uses a multi-layer stacked structure to realize a large area of ​​cell culture in a compact space. Compared with cell culture flasks, the number of cell inoculation has been expanded, and its role in large-scale cell culture has become increasingly prominent.