How to Watch Cells in the Cell Factory Systems

 In addition to paying attention to the changes in temperature, osmotic pressure, pH value and other conditions when culturing cells, it is also very important to grasp the growth status of cells. The cell factory systems are a multi-layer culture container, how should we observe the state of the cells?

1. Optical microscope observation: The morphology, structure and certain organelles of cells in the cell factory systems can be observed using an optical microscope. By adjusting the magnification and focal length of the microscope, images of different magnifications can be obtained to observe the details of cells.

2. Staining observation: By using cell staining technology, specific structures or components of cells can be displayed in color for easy observation. Commonly used staining methods include fluorescent staining and nucleic acid staining. Fluorescent staining can be used to observe the distribution and localization of specific markers through fluorescence microscopy, while nucleic acid staining can be used to observe the morphology of the nucleus and chromosome organization.

Cell Factory SystemsCell Factory Systems

3. Cell culture observation: Put the cells into a petri dish, provide proper nutrition and environmental conditions in the culture medium, and observe the growth and morphological changes of the cells. An inverted microscope can be used to observe the dynamic changes of living cells in a culture dish, and record the characteristics of cell proliferation, migration and cell cycle.

4. Observation of cell markers: By using specific cell markers, such as fluorescent proteins or fluorescent probes, specific cellular components or organelles can be marked, so as to observe their distribution and function in cells. For example, fluorescent probes can label organelles such as the nucleus, mitochondria, and endoplasmic reticulum, thereby helping to understand cell structure and function.

5. Electron microscope observation: Electron microscopes can provide higher-resolution images to observe finer cell structures. By processing and fixing cell samples, and then using an electron microscope to observe the ultrastructure of cells, such as cell membranes, endoplasmic reticulum, mitochondria, etc.

These approaches can be used alone or in combination to gain a deeper understanding of the structure, organization and function of cells in the cell factory systems. Choose the appropriate observation method according to your needs, and make sure that proper experimental procedures and safety measures are taken before conducting the observation.

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