Analysis of the causes of cell clumping in cell culture flasks

 Cultivating cells is a very rigorous task. In addition to creating a good growth environment for cells, it must also promptly resolve various emergencies during the culture process. Cell clumping in cell culture flasks is a phenomenon often encountered. What is the reason for this situation?

The clumping phenomenon of cells in the cell culture flasks can generally be analyzed from the following aspects:

1. The digestion process may be too rough, pipetting too hard, digestion for too long, too strong or toxic digestive juice, etc., will cause cell death. After the cell dies, it releases DNA, entangles other cells, and forms a sticky cell cluster.

2. Excessive cell centrifugation speed or too long time can also easily cause cell clumping.

3. When the digestion is not complete, the connection between the cells and the cell is not separated; when the digestion is over, the round cells tend to pile up and it is difficult to separate again.

4. Cells that are in poor condition and aging may also grow in clumps (such as poor cell condition caused by delay in changing the medium, too long to pass through, pollution, etc.).

Analysis of the causes of cell clumping in cell culture flasks

5. There is no pipetting evenly when passaging, and there are many cell clumps, which will be a clump when cultured.

6. Uneven inoculation or undigested cells.

7. Non-vibration culture or too much bacteria (cells) will form clumps.

If the cells in the cell culture flasks appear clumping, you can analyze the above aspects to find out the reason, and formulate strict procedures before the operation to avoid this phenomenon.

评论

发表评论

此博客中的热门博文

Two commonly used materials for carboys

图片
  In biological experiments, the processing and preservation of some liquids are often involved.   Carboys   are ideal for storing sterile liquids, distributing solutions and culture media. The commonly used specifications are mainly 10L and 20L. The material of this container are two main types: PP (polypropylene) and PC (polycarbonate). Polypropylene: It is a polymer formed by the addition polymerization reaction of propylene. The operating temperature range is -30~140℃. It can resist the corrosion of acids, alkalis, salt liquids and various organic solvents below 80℃, and can decompose under high temperature and oxidation. Polypropylene is a thermoplastic synthetic resin with excellent properties. It is a colorless, translucent thermoplastic lightweight general-purpose plastic with chemical resistance, heat resistance, electrical insulation, high-strength mechanical properties and good high-wear resistance processing properties.   Polycarbonate: The liquid storage...
阅读全文

What characteristics do the culture media bottles need to meet?

图片
  The media is the basic substance that provides cell nutrition and promotes cell proliferation, and is also the living environment for cell growth and reproduction. There are many types of medium, the raw materials for preparation are different, and the requirements for use are different. What characteristics should the   media bottles   meet to ensure that the medium is well preserved? The general medium is easily contaminated or decomposed by bacteria after being heated and absorbing moisture. Therefore, the general medium must be kept in a cool and moisture-proof place. For some media (such as tissue culture medium) that need to be strictly sterilized, they must be stored in a refrigerator at 3-6 °C for a long time. To ensure good storage of the medium, the media bottle should meet the following characteristics: 1. Sterility: The medium bottle must be aseptically treated to ensure that the medium is not contaminated. The general sterilization method will choose electr...
阅读全文

Cause Analysis of Mycoplasma Contamination in Cell Factory

图片
  Mycoplasma contamination of cells is a very troublesome problem when culturing cells, especially in the use of large-scale culture vessels such as   cell factory . Once mycoplasma contamination occurs, the loss will be even greater. So, what is the cause of mycoplasma contamination? Mycoplasma is a prokaryotic organism with a size of only 0.2-0.3 um, no cell wall, and can pass through a general filter membrane (0.22-0.45 um). During cell culture, the incidence of mycoplasma infection reaches 63%, so during cell culture Contamination by mycoplasma is a worldwide problem. When cells (especially passage cells) are contaminated by mycoplasma, the expression of DNA, RNA and protein in the cells will change, but the growth rate of cells generally does not have a significant impact, so the contamination of cells by mycoplasma is generally difficult to detect. In most cases, the cytopathic changes are slight or insignificant, and subtle changes can also be alleviated by subculture a...
阅读全文