What characteristics do the culture media bottles need to meet?

 The media is the basic substance that provides cell nutrition and promotes cell proliferation, and is also the living environment for cell growth and reproduction. There are many types of medium, the raw materials for preparation are different, and the requirements for use are different. What characteristics should the media bottles meet to ensure that the medium is well preserved?

The general medium is easily contaminated or decomposed by bacteria after being heated and absorbing moisture. Therefore, the general medium must be kept in a cool and moisture-proof place. For some media (such as tissue culture medium) that need to be strictly sterilized, they must be stored in a refrigerator at 3-6 °C for a long time. To ensure good storage of the medium, the media bottle should meet the following characteristics:

1. Sterility: The medium bottle must be aseptically treated to ensure that the medium is not contaminated. The general sterilization method will choose electron beam sterilization to meet the requirements of no DNase, no RNase, and no heat source;

2. Sealing: good sealing can protect the medium from the influence of the external environment;

3. Material: Some culture media need to be stored at low temperature, so the material of the culture medium bottle needs to have good low temperature resistance; in order to facilitate the observation of the capacity of the culture medium, the material of the culture medium bottle needs to have good transparency, and a scale should be marked on the bottle body .

The above are some requirements that the media bottle needs to meet. In addition, the medium, as a necessary substance for cell culture, involves a lot of manual operation processes. Therefore, the bottle should be designed to meet the basic principles of ergonomics as much as possible.