LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  Subculture refers to a process in which the culture needs to be divided into small parts, re-inoculated into another culture vessel (bottle), and then cultured.   Cell culture flasks   are commonly used consumables when culturing cells. So, how to passage cells in the cell culture flasks? Generally, the cells in the cell culture flask are basically saturated after they grow into a dense monolayer. In order to enable the cells to continue to reproduce and expand the number of cells at the same time, a passaging operation is required. Suspension cells can be directly divided into flasks, while adherent cells need to be digested before they can be divided into flasks. Adherent cells are generally digested into single cells using proteolytic enzymes (such as trypsin), and ethylenediaminetetraacetic acid (EDTA) can also be added to improve digestion. Observe the cells until the cells become round and detach from the flask wall. This process usually takes 5-15 minutes. To avoid cell clumpi

  Cell culture flasks   are a kind of consumables that are widely used in cell culture experiments. They can be used not only for cell recovery and small-scale cell expansion, but also for the expansion of adherent cells. With the continuous improvement and improvement of processing technology, these consumables are also constantly innovating to better meet the needs of researchers. 1. From the perspective of culture area, cell culture square flasks are available in 25cm2, 75cm2, 175cm2, 225cm2 and other specifications, which can meet the needs of various small and medium-scale cell culture. 2. Bottleneck design: The bottle neck of this consumable adopts a torticollis design, which is convenient for the import and export of culture medium. The neck is wide and has no dead space for easy access to the growth surface for pipettes and cell scrapers. 3. Two types of bottle caps: two types of breathable caps and sealed caps, which can be used in different culture environments. There are CO2

  When we use cell culture consumables such as   cell factories   to cultivate cells, we may find that there is a lot of cell contamination, which are: bacterial contamination, fungal contamination, mold contamination, and mycoplasma contamination. Bacterial contamination is generally caused by Bacillus and Staphylococcus. The main feature of contamination is that the medium in cell culture consumables such as cell factories turns yellow and turbid, and under low magnification, the medium is sandy and filled with intercellular spaces or culture medium, which can be seen under high magnification. Rod- or globular-shaped bacteria swim in the medium (notably different from the Brownian motion of cell debris). It should be noted that in the early stage of pollution, bacteria often exist in groups, and there may not be obvious movements. At this time, vigilance should be aroused. FuDau 10 Layers Cell Factory The common fungal contamination is Candida albicans contamination. After contaminat

  Cell factories   are suitable for industrial batch production, laboratory operations, and large-scale cell culture, and are ideal for adherent cells. As a cell culture consumable, what is its production process and how to control its quality? 1. Raw materials: High-quality raw materials are the basis for ensuring products. The cell factory uses USP Class VI compliant polystyrene to ensure batch-to-batch quality stability and consistency. 2. Injection molding: This consumable is produced by injection molding process, and is produced in a special 10,000-level clean room. After rigorous verification (planktonic bacteria, sedimentation bacteria, and suspended particle detection),  q uality management is carried out in accordance with the GMP workshop to ensure stable product quality. 3. Surface treatment: The polystyrene material itself is hydrophobic, so it must undergo surface treatment and introduce hydrophilic groups in order to better adapt to the growth of adherent cells.          

  Cell factory   play an important role in vaccine production, monoclonal antibodies and the pharmaceutical industry, and are mostly used for the culture of adherent cells. Compared with other cell culture vessels, it has the following advantages: Small footprint: The cell factory adopts a multi-layer structure design, also known as a multi-layer cell incubator. Common specifications include 1 layer, 2 layers, 5 layers, 10 layers, 40 layers, etc. To expand the culture scale, you only need to increase the number of layers of the product That is, it will not take up too much space, which can save a lot of plant space for enterprises, and reduce the cost of quality control and downstream purification of their enterprises. Reduce pollution: This container is equipped with a full set of accessories, including liquid transfer cover, small port conversion cover, CPC adapter, tee pipeline, silicone tube/hot melt tube, ECS quick connector, etc. Through the mutual cooperation of different pipeli

  Cell culture flasks   are a kind of cell consumables that are often used in the process of cell culture. When culturing cells, various contaminations often cause various troubles to the experiment and hinder the experimental process. So how to deal with these pollution? Cell contamination is generally caused by improper aseptic technique, incomplete sterilization of vessel reagents, and passage of hands or instruments over the open vessel mouth. Once the cells are contaminated, most of them are difficult to recover. The following are the common types and treatment methods of cell contamination: 1. Bacterial contamination: Bacteria are black and fine sand-like under an ordinary inverted microscope. Depending on the infected bacteria, they may have different shapes. The culture medium will generally become cloudy and yellow, which has a significant impact on cell growth. Most of the cells die within 24 hours. Corresponding antibiotic treatment, such as tetracycline, gentamicin, etc., c

  There are many problems when using   cell culture flasks   to culture cells, such as cell non-adherence, cell contamination, cell death, etc. Among them, precipitation in cell culture flasks is also a common situation, which may be related to metal supplements. Metal ions such as copper, iron, and zinc are required for cell growth and are important supplements to serum-free media. The absence of other serum components in the culture system may lead to the precipitation of these metals, creating a toxic environment for the cells. At higher pH (generally >8), carbonate and hydroxide ions and copper ions, phosphate and hydroxide ions and iron ions, carbonate, phosphate and hydroxide ions and zinc ions , hydroxide ions and magnesium ions are easy to form insoluble precipitates; under oxidative conditions, copper and zinc are more likely to precipitate, which is the precipitate we see in cell culture flasks. So how do we avoid the precipitation caused by metal supplements? We can add i

  When culturing cells,   cell culture flasks   are a kind of consumable with high usage rate. When culturing cells, they will experience four different growth phases, namely stopper, logarithmic phase, stationary phase and decline phase. These four phases reflect the different growth states of cells. Arrest phase: The phase in which cells adapt to the culture conditions in the cell culture flask, during which the cells do not divide. Cells typically attach within 24 hours of starting the culture, and the total length of this phase depends on the growth phase and seeding density at which the cells were used at the beginning of the culture.    FuDau T25 Cell Culture Flasks Log phase: Cells are actively dividing during this phase and are the optimal time to assess population growth and general data collection. Late in log phase, before cell stress due to overcrowding is the best time to pass cells (subculture). Stationary phase: As cells reach 100% confluence, cell growth slows down at t

  In the process of culturing cells in   cell culture flasks , we encounter various conditions, and slow cell growth is one of them. So, what is the reason for this and how to fix it? There are many reasons for slow cell growth, including the following: 1. Due to the replacement of different culture medium or serum; 2. Some components necessary for cell growth in the culture medium, such as glutamine or growth factors, are depleted or lacking or have been destroyed; 3. There is a small amount of bacterial or fungal contamination in the culture; 4. Improper storage of reagents; 5. The initial concentration of inoculated cells is too low; 6. Cells have aged; 7. Mycoplasma contamination. solution: 1. Compare the composition of the new medium and the original medium, compare the new serum and the old serum to support cell growth experiments, and let the cells gradually adapt to the new medium; 2. Change into freshly prepared culture medium, or add glutamine and growth factors; 3. Use antib

  When culturing cells, the   cell culture flask   is a kind of cell consumable with a relatively large amount. Cultivating cells is a very rigorous job, and it requires the right conditions for better growth. The harvest of the cells involved in the growth to a certain extent, then, how to better digest the cells in the cell culture flask, you can follow the following four steps: 1. The first step: pour out the old medium and add a small amount of new medium to wash 1-2 times. The purpose of this is to wash away the floating dead cells as much as possible. 2. Step 2: Add a small amount of new medium and pipet it directly, this time is to blow down the cells that are not firmly attached. After another wash with medium, the resulting suspension was mixed twice and transferred to a new cell culture flask. 3. Step 3: Aspirate the remaining liquid in the bottle, add about 0.3ml of trypsin to rinse it once, suck it off and discard it, and then add about 1ml of trypsin to digest. While diges