How to better digest cells in cell culture flasks

 When culturing cells, the cell culture flask is a kind of cell consumable with a relatively large amount. Cultivating cells is a very rigorous job, and it requires the right conditions for better growth. The harvest of the cells involved in the growth to a certain extent, then, how to better digest the cells in the cell culture flask, you can follow the following four steps:

1. The first step: pour out the old medium and add a small amount of new medium to wash 1-2 times. The purpose of this is to wash away the floating dead cells as much as possible.

2. Step 2: Add a small amount of new medium and pipet it directly, this time is to blow down the cells that are not firmly attached. After another wash with medium, the resulting suspension was mixed twice and transferred to a new cell culture flask.

3. Step 3: Aspirate the remaining liquid in the bottle, add about 0.3ml of trypsin to rinse it once, suck it off and discard it, and then add about 1ml of trypsin to digest. While digesting, place it under a microscope for observation. When the gap between cells is obvious, immediately aspirate and discard the trypsin, add new medium and start pipetting. After pipetting 2-3 times, pipette the suspension into a test tube or new bottle for temporary storage. Wash it with about 2ml of new medium and mix it with the previous one.

4. Step 4: Add new trypsin into the flask to continue digesting the remaining firmly adherent cells. Continue to observe under the microscope. When the remaining cell gap is obvious and the cells are independent, aspirate the trypsin, add new medium and pipet, and transfer the suspension to a new bottle for culture.

Through the above operations, the quality of the cells digested from the cell culture flask is better, and there will be a better growth state in the later passage process.