These factors affect cell growth in cell culture flasks

 The cell culture flask is a square, wide-necked cell culture container, which is a commonly used consumable for adherent cell culture. The growth of cells is affected by a variety of factors, the most common of which are the following four:

1. The state of the cell itself: the state of the cell itself is an important factor affecting the growth of the cell. If the number of passages is too many, it is easy to age the cells and affect the later proliferation. When using trypsin for cell digestion in the later stage, the time should be well controlled. If the time is too short or too long, it will affect the later growth of cells.

FuDau T25 Cell Culture Flasks

2. Bacterial contamination: Cell growth requires a sterile environment. If the cells in the cell culture flask are contaminated by mycoplasma or mold, the cells will grow slowly or even die. Therefore, aseptic operations should be strictly followed when culturing cells.

3. Medium or serum: These two solutions provide cells with nutrients needed for growth, but different cells have different requirements for medium or serum. Selecting a suitable medium according to cell characteristics can ensure the normal growth of cells in the later stage.

FuDau T75 Cell Culture Flasks

4. Culture environment: The gas environment is also one of the important conditions for cell growth. Whether the supply of carbon dioxide is normal will also affect the growth state of cells.

In short, there are many factors that affect the growth of cells in cell culture flasks, and the above are the common ones. In addition, if it is adherent cells, TC-treated consumables should be selected, which can allow the cells to grow better.