Analysis of the Causes of Precipitation in Cell Culture Flasks

Cultivating cells is like raising children. Care must be taken to care for them. A little carelessness may affect the growth of cells. Precipitation of the culture medium in the cell culture flask is a common problem. What is the reason for this and how can we deal with it?

           FuDau T25 Cell Culture Flasks

In fact, there are two main reasons for the precipitation of the solution in the cell culture flask. On the one hand, the cells themselves are contaminated, and on the other hand, the culture medium contains metals, proteins and other medium components. From the perspective of cell contamination, common cell contaminations include bacterial and fungal contamination, mycoplasma contamination, eukaryotic cell contamination, etc. After contamination, the culture solution will turn yellow and precipitate. At this time, it is recommended to treat the culture immediately. Re-establish the culture.

Another reason is the composition of the medium itself. If after investigation, it is determined that there is no contamination, it is likely to be the precipitation of metals, proteins and other media components in the cell culture medium. These precipitates may affect the health of the cells, because they generally remove nutrients and other nutrients through chelation. required components, thereby changing the composition of the medium. Changes in temperature, concentration changes induced by water loss, calcium salts, metal supplements, etc., are all likely to cause precipitation of the medium.

        FuDau T75 Cell Culture Flasks

In short, if there is precipitation in the cell culture flask, there may be many reasons. We must investigate carefully and find out the specific reasons to solve the problem.