LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  Cell factory   culture technology is one of the effective methods for large-scale cell culture and virus amplification. It has the advantages of low risk of contamination, convenient amplification, and space saving. Oral rotavirus culture is one aspect of its application. Rotavirus is one of the main pathogens that cause diarrhea in infants and young children. It mainly infects small intestinal epithelial cells, causing cell damage and diarrhea. The infection route is the fecal-oral route, and the clinical manifestations are acute gastroenteritis, osmotic diarrhea, and dehydration symptoms in severe cases. Vaccination against rotavirus in infants and young children is the main measure to prevent the disease. Compared with the traditional roller bottle culture, the cell factory culture technology is an efficient cell culture container. Its surface is specially treated to have uniform and continuous cell adsorption, which can effectively promote the adherent growth of cells and shorten

Cell culture is an important and commonly used technique in cell biology research methods. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cell anabolism, cell growth and proliferation can be studied by means of cell culture. In large-scale cell culture,   cell factory   is an efficient and commonly used cell culture consumable. The cell factory consists of one or more layers, the common ones are 1 layer, 2 layers, 5 layers, 10 layers, 40 layers, etc. The layers are connected by a special adhesive process to ensure the firmness of the product. In order to improve the hydrophilicity of the container surface and make it more suitable for cell growth and reproduction, this consumable will undergo special surface treatment to introduce hydrophilic groups on the hydrophobic surface to enhance the adherence of cells and make it easier for cells with low adhesion characteristics stick to the wall. The cell factory is mainly suitable for the culture

  A   cell factory   is a commonly used consumable in large-scale cell culture. Its structure consists of one or more layers, and the uppermost layer is provided with two perfusion ports. What process is used to produce such a complex structure? The injection molding process adopted by the cell factory mainly includes the following steps: ①Pre-molding process: The pre-molding process is that the plastic material is heated, transported, compacted, sheared, mixed and homogenized in the barrel to transform the material from a glass state to a viscous fluid state, so as to meet the injection molding requirements. ②Injection filling process: In the injection filling stage, the screw injects the melt in the storage chamber into the cavity through the nozzle, the mold runner and the gate under the thrust of the injection cylinder. ③ Compression and compression process: Continue injection molding to fill the volume vacated by the part due to shrinkage. ④Cooling and setting process: After the h

The   cell factory   is mainly suitable for the large-scale culture of adherent cells. Generally, there are two types of caps: breathable caps and airtight caps, which can be used in different culture environments. There will be a waterproof and breathable membrane on the bottle cap, which plays an important role in the cell culture process. The waterproof breathable membrane on the bottle cap of the cell factory is a new type of polymer waterproof material. From the production process, the technical requirements of the waterproof breathable membrane are much higher than that of ordinary waterproof materials; at the same time, from the quality point of view, the waterproof breathable membrane It also has functional characteristics that other waterproof materials do not have. 1. High temperature resistance - the working temperature is up to 250 ℃. 2. Low temperature resistance - good mechanical toughness; 5% elongation can be maintained even if the temperature drops to -196℃. 3. Corrosi

  With the continuous innovation and rapid development of biotechnology, regenerative medicine and cell therapy have experienced explosive growth, and large-scale cell culture technology has continued to mature. Among them, the   cell factory   is a commonly used consumable in large-scale cell culture, and this cell culture method generally requires the aid of a shaker. At present, common large-scale cultured animal cells include CHO, Vero, MRC-5, 2BS, 293T, L929 and human diploid cells. These cells are mainly used in erythropoietin, monoclonal antibody preparation, vaccine production and other products. The large-scale culture of animal cells in vitro requires high-density mass production in cell culture containers under the conditions of artificially set temperature, humidity, pH and dissolved oxygen. Among many large-scale culture methods, cell factories have the characteristics of saving space, reducing labor input, and avoiding pollution, and are gradually favored by scientific re

Large-scale cell culture technology refers to the technology of artificially controlling environmental conditions and cultivating useful microorganisms, animal and plant cells on a large scale with high density. It is the basis for a large number of new cells obtained by gene recombination, cell fusion and other technologies in industrial production such as food and medicine.   Cell factories   are commonly used consumables in large-scale cell culture. The common layers of cell factories include 1 layer, 2 layers, 5 layers, 10 layers, 40 layers, etc., which are mainly suitable for the culture of adherent cells. To expand its training scale, it can be achieved by increasing the number of layers. This training method can maximize the utilization of large space, save plant space, and save manpower and material resources. In industry, cell factories are widely used in mass production of vaccines, monoclonal antibodies or the pharmaceutical industry. Common animal cells that have been cultu

The   cell factory   is one of the commonly used consumables in the field of biological research, suitable for industrial mass production and large-scale cell culture, such as vaccines, monoclonal antibodies or the pharmaceutical industry. Cells are very sensitive to the environment, so sterilization of consumables such as cell factories is an essential process before use. Commonly used sterilization methods include ethylene oxide sterilization, high pressure steam sterilization, irradiation sterilization, etc. The sterilization method of the cell factory selects a relatively special electron beam sterilization, which belongs to one of the irradiation sterilization methods. Electron beam sterilization is the use of electron beam rays to destroy the DNA of product microorganisms, so as to effectively inhibit the reproduction of microorganisms, disinfect and sterilize, and prevent mildew, insects, and preservatives. This sterilization method has the following characteristics: 1. Pure phy

  With the rapid development of the biopharmaceutical industry, the use of cell culture technology to conduct research on vaccines, monoclonal antibodies and other drugs has become a trend.   Cell factories   are commonly used consumables in large-scale cell culture, which play an important role in the large-scale production of varicella vaccines. Chickenpox is a highly contagious acute infectious disease caused by the varicella-zoster virus. The clinical symptoms are fever, mainly macules, papules, vesicles and scabs in batches, and even death in severe cases. The chickenpox vaccine is the safest and most effective way to prevent chickenpox. The varicella vaccine adopts the attenuated strain of varicella virus OKa, which is commonly used in the world, and is made by MRC-5 human diploid cell culture. The appearance of the freeze-dried product is a milky white loose body, and it is a pale yellow liquid after dissolving. Getting the chickenpox vaccine not only protects against chickenpox

Cell culture refers to a method in which cells survive, grow, reproduce and maintain their main structures and functions in a certain growth environment.   Cell factories   are often used for large-scale cell culture, and their caps vary depending on the environment in which they are used. The growth environment of cells includes sterility, suitable temperature (37 ～ 38℃), osmotic pressure (260 ～ 320mmol/L), carbon dioxide and suitable pH (7.2 ～ 7.4). Cell factories generally need to use incubators and greenhouses for cell culture. According to the different use environments, the bottle caps are divided into two types: sterilizing and ventilating caps and sealing caps. Sterilization and breathable cover: This cover has ventilation holes on the top and is mainly used in a carbon dioxide environment. The breathable cover can allow carbon dioxide in the environment to enter the cell factory and create suitable growth conditions for cell growth. In addition, this lid also has a sterilizati

  In recent years, cell culture technology has been maturing, and   cell factories   have been increasingly used in large-scale cell culture, among which freeze-dried live attenuated hepatitis A vaccine is an important branch of its application field. Hepatitis A, referred to as hepatitis A, is an acute infectious disease caused by the hepatitis A virus (HAV). The clinical manifestations are acute onset, with chills, fever, loss of appetite, nausea, fatigue, hepatomegaly and abnormal liver function. Jaundice occurs in some cases, and asymptomatic infections are more common, and generally do not become chronic and pathogen-carrying. Hepatitis A vaccine is a vaccine used to prevent hepatitis A. It has become one of the main vaccines for children in China. In May 2008, it was included in one of the expanded immunization vaccines. Some provinces and cities have provided free hepatitis A vaccination. The hepatitis A vaccines on the market mainly include inactivated hepatitis A vaccines and

  The   cell factory   is a multi-layered cell culture consumable, which is mostly used in industrial mass production, laboratory operations and large-scale cell culture, and is an ideal choice for adherent cells. When choosing this kind of consumables, we will pay attention to a key factor, that is the surface TC treatment, why does it have to undergo this special treatment? Cells can be divided into two types: adherent cells and suspension cells according to different growth methods. Suspension cells do not need to be attached to a support to grow, and can grow in suspension in a culture medium. Adherent cells must have a growth surface to which they can attach to grow, and only a hydrophilic solid interface can allow cells to attach. The cell factory is mainly used for the culture of adherent cells, which is processed by injection molding of transparent polystyrene (PS) raw materials. As a polymer material, PS has good strength and plasticity, and has no toxicity, and has become the

  Cell factories   are commonly used consumables in large-scale cell culture, and are widely used in adherent cells. It is processed from raw polystyrene, which is inherently hydrophobic, and adherent cells need to attach to the surface of the consumable to grow. In order to improve the adherence performance of the product, it will be treated with TC on the surface to introduce hydrophilic groups to meet the growth requirements of adherent cells. So, how to detect the TC treatment effect of consumables? The untreated polystyrene surface is uncharged and non-hydrophilic, and its ability of cell adsorption and protein binding is very poor, so that cell adsorption and growth are very weak and unstable. After TC treatment, it is a negatively charged and hydrophilic polystyrene surface, and its ability of cell adsorption and protein binding is very stable, which is suitable for cell adherent culture. To detect the TC treatment effect of the cell factory, a professional instrument - a contac

The   cell factory   is a multi-layer structure of cell culture consumables. Different from cell culture flasks, culture plates and other consumables, this kind of container is relatively complicated in operation, so how to use it specifically. Cell factories are generally used to expand the culture of cells. First, the number of cells to be seeded needs to be determined according to the culture area of ​​the factory before operation. According to the number of cells cultured per unit area in the cell flask, multiply by the culture area of ​​the cell factory, and then divide by the inoculation ratio of cultured cells. Next, prepare the working solution required for cell culture, such as serum, protease, etc. After the preparations are done, you can inject the liquid into the container and pour it directly through the liquid inlet. Pay attention to controlling the liquid flow rate to avoid generating too many air bubbles. After the liquid injection is complete, turn the container to one

The sensitization test is a test to determine whether the test substance is an allergen and the sensitization intensity. As a cell consumable commonly used in large-scale cell or tissue culture,   cell factories   also need to be subjected to sensitization tests to determine whether they contain allergens and sensitization strength. The phenomenon in which the specific sensitivity of the body to an antigenic or haptenic chemical is increased. Chemicals that have a sensitizing effect are called chemoallergens or chemosensitizers. Chemical sensitizers act on the body through various ways, and can make immune active cells in the body produce specific immune responses to them. symptoms and signs. If the cell factory contains allergens, it will affect the growth and proliferation of cells. The sensitization test animals are mostly guinea pigs, and the test substances are mostly pure chemical products. The dose to be used is determined experimentally, starting with the maximum dose that does

  Cytotoxicity is a purely cell-killing event caused by cells or chemicals that does not rely on apoptosis or necrosis as a cell death mechanism. Through this test, it can be judged whether the raw materials of the   cell factory   contain substances that are not conducive to cell growth. Cytotoxicity is the effect of chemical substances (drugs) on the basic structure and/or physiological process of cells, such as cell membrane or cytoskeleton structure, cell metabolism process, synthesis, degradation or release of cell components or products, ion regulation and cell division and other processes , leading to disorders of cell survival, proliferation and/or function, resulting in adverse reactions. Generally, testing whether a cell factory is cytotoxic can be done by the following methods: 1. MTT and XTT methods: using the activity of enzymes inside the mitochondria, specific tetrazolium salts can be converted, and then detected by a microplate reader; 2. LDH method: cytotoxicity is det

The   cell factory   is a multi-layer structure of cell culture consumables, which has the advantages of large culture area, small plant space occupation, and low pollution degree. In order to ensure the quality of the product, various tests are required, and the initial contamination bacteria test is one of them. Generally speaking, the initial contamination bacteria test is the estimated value of the bioburden of the product to be sterilized. It is a microbial limit test, which refers to the degree of microbial contamination of non-specified sterilized products and raw materials, auxiliary materials and finished products before sterilization, including the amount of contamination and control bacteria. , that is, the usual colony count, coliform and pathogenic bacteria inspection. The purpose of this test is to provide a reference index for the next sterilization of the cell factory, such as the sterilization dose, so as to ensure the sterilization effect. It can be said to be a test

Testing the quality of cell consumables is an important way to ensure their quality. As a kind of commonly used consumables in large-scale cell culture,   cell factories   must also pass professional testers to ensure product quality. The process testing items involved include the following: kind: 1. Performance verification of injection molding machine and mold: The cell factory is made of polystyrene raw materials through injection molding process. The performance of injection molding machine and mold determines the quality of the finished product. Therefore, the performance of injection molding machine and mold must be verified before production. 2. Performance verification of surface treatment equipment: This cell consumable is mainly suitable for the culture of adherent cells. In order to make the cells better adhere to the surface of the consumable, surface treatment is generally required to improve the hydrophilicity of the material and allow the cells better growth. 3. Performa

The physical and chemical testing of the   cell factory   is a very important aspect of the quality testing of the cell factory. It involves a number of testing items, mainly including residues on ignition, leachables testing, metal elements, etc. These tests are mainly to determine whether the raw materials contain influence Cell growth chemicals, thus ensuring the safety of cell culture. Residue on ignition: After the organic drug is decomposed by carbonization or inorganic drug heating, add sulfuric acid to moisten it, and then ignite at low temperature and then at high temperature (700-800°C), so that it is completely ashed, the organic matter is decomposed and volatilized, and the residual non-volatile inorganic impurities (more Metal oxides or inorganic salts) become sulfates, known as residues on ignition. This test is mainly to detect whether the impurities in the production raw materials of the cell factory exceed the standard, so as to judge the purity of the raw materials. D