Specific steps used in cell factories

The cell factory is a multi-layer structure of cell culture consumables. Different from cell culture flasks, culture plates and other consumables, this kind of container is relatively complicated in operation, so how to use it specifically.

Cell factories are generally used to expand the culture of cells. First, the number of cells to be seeded needs to be determined according to the culture area of ​​the factory before operation. According to the number of cells cultured per unit area in the cell flask, multiply by the culture area of ​​the cell factory, and then divide by the inoculation ratio of cultured cells. Next, prepare the working solution required for cell culture, such as serum, protease, etc.

After the preparations are done, you can inject the liquid into the container and pour it directly through the liquid inlet. Pay attention to controlling the liquid flow rate to avoid generating too many air bubbles. After the liquid injection is complete, turn the container to one side so that the liquid is evenly distributed between the layers. The container is then tilted toward the end with no inlet/outlet to distribute the liquid evenly between each layer. In the process of tilting, it is necessary to ensure that the side of the cells entering the liquid is raised at the same time, and it is necessary to avoid the flow of liquid between the layers and destroy the distribution between each layer. Finally, lay the container flat and place it in a suitable culture environment.

In the process of operation, it should be noted that when moving the cell factory, try to keep it as level as possible to avoid uneven distribution of liquid caused by tilting, which will affect the cell growth effect.