The difference between the sealing cap and the breathable cap of the cell culture flasks

The cell culture flasks is a kind of cell culture consumable, which plays an important role in the medium-scale cell and tissue culture in the laboratory. The bottle cap of this kind of bottle is divided into two types: a sealed cap and a breathable cap. Then the two caps are respectively, what kind of scenario is applicable and what is the difference?

The cell culture environment includes sterile, suitable temperature (37～38℃), osmotic pressure (260～320mmol/L), carbon dioxide and suitable pH (7.2～7.4). Cell culture square flasks generally require the help of incubators or greenhouses for cell culture. According to the different use environments, the lids are divided into two types: sealed lids and breathable lids.

The difference between the sealing cap and the breathable cap of the cell culture flasks

Sealing cap: This kind of cap is completely sealed. There are no vents on the cap. It is mainly used under carbon dioxide-free conditions such as incubators and greenhouses. It has good airtightness and can prevent the invasion of external bacteria and promote cell reproduction. Create a good growth environment.

Breathable cover: This kind of cover is equipped with vent holes, these holes can let the carbon dioxide in the environment enter the cell culture square flask, and create suitable growth conditions for cell growth. There is a sterile breathable membrane on the top of the bottle cap, which has good waterproof and breathable functions. The liquid in the cell culture square bottle does not affect the microbial barrier and breathability of the breathable membrane after contact, which can ensure a good growth state of cells.

The two caps of the cell culture flasks meet the needs of different culture environments for cell growth. When choosing the cell culture flasks, you should choose a suitable cap according to the specific cell culture environment to avoid affecting cell growth.

评论

发表评论

此博客中的热门博文

Two commonly used materials for carboys

图片
  In biological experiments, the processing and preservation of some liquids are often involved.   Carboys   are ideal for storing sterile liquids, distributing solutions and culture media. The commonly used specifications are mainly 10L and 20L. The material of this container are two main types: PP (polypropylene) and PC (polycarbonate). Polypropylene: It is a polymer formed by the addition polymerization reaction of propylene. The operating temperature range is -30~140℃. It can resist the corrosion of acids, alkalis, salt liquids and various organic solvents below 80℃, and can decompose under high temperature and oxidation. Polypropylene is a thermoplastic synthetic resin with excellent properties. It is a colorless, translucent thermoplastic lightweight general-purpose plastic with chemical resistance, heat resistance, electrical insulation, high-strength mechanical properties and good high-wear resistance processing properties.   Polycarbonate: The liquid storage...
阅读全文

What characteristics do the culture media bottles need to meet?

图片
  The media is the basic substance that provides cell nutrition and promotes cell proliferation, and is also the living environment for cell growth and reproduction. There are many types of medium, the raw materials for preparation are different, and the requirements for use are different. What characteristics should the   media bottles   meet to ensure that the medium is well preserved? The general medium is easily contaminated or decomposed by bacteria after being heated and absorbing moisture. Therefore, the general medium must be kept in a cool and moisture-proof place. For some media (such as tissue culture medium) that need to be strictly sterilized, they must be stored in a refrigerator at 3-6 °C for a long time. To ensure good storage of the medium, the media bottle should meet the following characteristics: 1. Sterility: The medium bottle must be aseptically treated to ensure that the medium is not contaminated. The general sterilization method will choose electr...
阅读全文

Cause Analysis of Mycoplasma Contamination in Cell Factory

图片
  Mycoplasma contamination of cells is a very troublesome problem when culturing cells, especially in the use of large-scale culture vessels such as   cell factory . Once mycoplasma contamination occurs, the loss will be even greater. So, what is the cause of mycoplasma contamination? Mycoplasma is a prokaryotic organism with a size of only 0.2-0.3 um, no cell wall, and can pass through a general filter membrane (0.22-0.45 um). During cell culture, the incidence of mycoplasma infection reaches 63%, so during cell culture Contamination by mycoplasma is a worldwide problem. When cells (especially passage cells) are contaminated by mycoplasma, the expression of DNA, RNA and protein in the cells will change, but the growth rate of cells generally does not have a significant impact, so the contamination of cells by mycoplasma is generally difficult to detect. In most cases, the cytopathic changes are slight or insignificant, and subtle changes can also be alleviated by subculture a...
阅读全文