博文

Several conditions necessary for PETG media bottles

图片
As an essential nutrient for cell growth, serum has a good protective effect on cells in culture, and the quality of serum directly affects the growth state of cells.   PETG media bottle   is a special container for storing serum, it must meet some necessary conditions to ensure the stability and safety of serum during storage. Low temperature resistance: The storage of serum is different from ordinary items, and generally needs to be stored in an environment of -5°C to -20°C. If stored at 4°C, try not to exceed one month. This requires that the media bottle must have good low temperature resistance to meet the low temperature storage requirements of serum. PETG Media Bottles Transparency: The quality of the serum, which we can initially discern by its appearance. A good serum should be transparent and clear, earthy yellow or brownish yellow, with no or very little precipitation, and relatively viscous. If the serum is found to be turbid, opaque, and contains many precipitates...

Three characteristics of cell culture flasks

图片
  When conducting cell culture experiments,   cell culture flasks   are a tool that we often use. Its specifications range from 25cm² to 225cm², which can meet the needs of different scales of cell culture. As a common tool, this consumable has the following three characteristics: 1. Torticollar design: The neck of the cell culture flask adopts a torticollar design, which makes it easier for pipettes and cell scrapers to enter the interior, and there is no dead angle in contact, which is convenient for researchers to operate. 2. Two kinds of covers: According to the different culture environment, this consumable has two kinds of covers, airtight cover and air-permeable cover. Caps are often used for closed cultures, but can also be used for open cultures when the caps are unscrewed. The breathable cover is suitable for open culture. The cover is equipped with a 0.2um hydrophobic filter membrane to avoid possible cross-infection during gas exchange. It is generally recomme...

How to Digest Cells from Cell Culture Flasks

图片
  Cell culture flasks   are mostly used for the cultivation of adherent cells. Different from the growth of suspension cells in the culture medium, adherent cells need to grow on the surface of the container, so the cells must be digested before harvesting or passage. There are many ways to digest cells, the common ones are as follows: 1. Enzyme digestion This is also a method that we use more in our daily life. Generally, trypsin is used for digestion, and the concentration is between 0.25% and 0.5%. The digestion time varies according to the cell type, operation method, temperature and other factors. After digestion, it is necessary to stop the digestion with serum or serum-containing medium. T25 Flask 2. Physical method Without the aid of trypsin, directly pipette or use a cell scraper to digest the cells on the surface of the cell culture flask. This method can be used for cells that are firmly attached. However, it should be noted that pipetting and scraping will cause ce...

FDA authorizes Gohibic (vilobelimab) injection for the treatment of COVID-19

图片
  FDA has issued an emergency use authorization (EUA) for the use of Gohibic (vilobelimab) injection for the treatment of COVID-19 in hospitalized adults when initiated within 48 hours of receiving invasive mechanical ventilation or extracorporeal membrane oxygenation (artificial life support). The treatment targets a part of the immune system that is thought to play a role in the inflammation that leads to COVID-19 disease progression. The clinical trial supporting the authorization showed that patients treated with Gohibic had a lower risk of death by day 28 and day 60 of treatment compared to placebo. PETG Media Bottles “COVID-19 treatments remain a priority for CDER, as the disease continues to impact the lives of Americans,” said Center Director Patrizia Cavazzoni, M.D. “Today's authorization offers another potentially life-saving treatment option for the sickest COVID-19 patients.” The recommended dosage of Gohibic is 800 mg administered by intravenous infusion after dilution...

The difference between the two culture surfaces of cell culture flasks

图片
  There are thousands of cell types, but they can be roughly divided into two categories: adherent cells and suspension cells. Different cells have different requirements for culture containers. As a consumable product with high frequency of use,   cell culture flasks   are divided into two types: TC-treated surface and non-TC-treated surface. So, what is the difference between these two surfaces? Cells are divided into adherent cells and suspension cells according to different culture methods. The growth of adherent cells must have a support surface that can be attached, and the cells can grow on the surface by relying on the attachment factors secreted by themselves or provided in the medium and reproduce. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes. T225 Flask The cell culture flask is made of polystyrene raw material. Compared with glass material, the surface of this material is hydrophobic, which is ...

The difference between the cell culture flask Sealed Cap and the Vent Cap

图片
  Cell culture flasks   are mainly used for adherent cell culture, and cell culture scale-up in laboratory or industrial production processes. According to the different culture conditions, this cell culture container has two types of lids: Sealed Cap and Vent Cap. So, what is the difference between these two types of caps?            FuDau T25 Cell Culture Flasks The sealing cap is completely sealed and is suitable for cell and tissue culture under sealed conditions, so that the culture environment is completely isolated from the outside world. The breathable cover has a hydrophobic filter membrane with a pore size of 0.22um, which can meet the needs of gas exchange in cell and tissue culture, and can effectively prevent cross-contamination. It is suitable for open culture conditions. The two types of caps for cell culture flasks are first used in different environments. The vent cap is suitable for use in an environment with carbon dioxide, whil...

The difference between the two culture surfaces of cell culture flasks

图片
  There are thousands of cell types, but they can be roughly divided into two categories: adherent cells and suspension cells. Different cells have different requirements for culture containers. As a consumable product with high frequency of use,   cell culture flasks   are divided into two types: TC-treated surface and non-TC-treated surface. So, what is the difference between these two surfaces? Cells are divided into adherent cells and suspension cells according to different culture methods. The growth of adherent cells must have a support surface that can be attached, and the cells can grow on the surface by relying on the attachment factors secreted by themselves or provided in the medium and reproduce. Suspension cells do not require an attachable support and grow in suspension in the medium, such as lymphocytes. T225 Flask The cell culture flask is made of polystyrene raw material. Compared with glass material, the surface of this material is hydrophobic, which is ...