LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  Cell factory   culture technology is one of the effective methods for large-scale cell culture and virus amplification. It has the advantages of low risk of contamination, convenient amplification, and space saving. Oral rotavirus culture is one aspect of its application. Rotavirus is one of the main pathogens that cause diarrhea in infants and young children. It mainly infects small intestinal epithelial cells, causing cell damage and diarrhea. The infection route is the fecal-oral route, and the clinical manifestations are acute gastroenteritis, osmotic diarrhea, and dehydration symptoms in severe cases. Vaccination against rotavirus in infants and young children is the main measure to prevent the disease. Compared with the traditional roller bottle culture, the cell factory culture technology is an efficient cell culture container. Its surface is specially treated to have uniform and continuous cell adsorption, which can effectively promote the adherent growth of cells an...

Cell culture is an important and commonly used technique in cell biology research methods. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cell anabolism, cell growth and proliferation can be studied by means of cell culture. In large-scale cell culture,   cell factory   is an efficient and commonly used cell culture consumable. The cell factory consists of one or more layers, the common ones are 1 layer, 2 layers, 5 layers, 10 layers, 40 layers, etc. The layers are connected by a special adhesive process to ensure the firmness of the product. In order to improve the hydrophilicity of the container surface and make it more suitable for cell growth and reproduction, this consumable will undergo special surface treatment to introduce hydrophilic groups on the hydrophobic surface to enhance the adherence of cells and make it easier for cells with low adhesion characteristics stick to the wall. The cell factory is mainly suitable for th...

  A   cell factory   is a commonly used consumable in large-scale cell culture. Its structure consists of one or more layers, and the uppermost layer is provided with two perfusion ports. What process is used to produce such a complex structure? The injection molding process adopted by the cell factory mainly includes the following steps: ①Pre-molding process: The pre-molding process is that the plastic material is heated, transported, compacted, sheared, mixed and homogenized in the barrel to transform the material from a glass state to a viscous fluid state, so as to meet the injection molding requirements. ②Injection filling process: In the injection filling stage, the screw injects the melt in the storage chamber into the cavity through the nozzle, the mold runner and the gate under the thrust of the injection cylinder. ③ Compression and compression process: Continue injection molding to fill the volume vacated by the part due to shrinkage. ④Cooling and setting proces...

The   cell factory   is mainly suitable for the large-scale culture of adherent cells. Generally, there are two types of caps: breathable caps and airtight caps, which can be used in different culture environments. There will be a waterproof and breathable membrane on the bottle cap, which plays an important role in the cell culture process. The waterproof breathable membrane on the bottle cap of the cell factory is a new type of polymer waterproof material. From the production process, the technical requirements of the waterproof breathable membrane are much higher than that of ordinary waterproof materials; at the same time, from the quality point of view, the waterproof breathable membrane It also has functional characteristics that other waterproof materials do not have. 1. High temperature resistance - the working temperature is up to 250 ℃. 2. Low temperature resistance - good mechanical toughness; 5% elongation can be maintained even if the temperature drops to -196℃. 3...

  With the continuous innovation and rapid development of biotechnology, regenerative medicine and cell therapy have experienced explosive growth, and large-scale cell culture technology has continued to mature. Among them, the   cell factory   is a commonly used consumable in large-scale cell culture, and this cell culture method generally requires the aid of a shaker. At present, common large-scale cultured animal cells include CHO, Vero, MRC-5, 2BS, 293T, L929 and human diploid cells. These cells are mainly used in erythropoietin, monoclonal antibody preparation, vaccine production and other products. The large-scale culture of animal cells in vitro requires high-density mass production in cell culture containers under the conditions of artificially set temperature, humidity, pH and dissolved oxygen. Among many large-scale culture methods, cell factories have the characteristics of saving space, reducing labor input, and avoiding pollution, and are gradually favored by...

Large-scale cell culture technology refers to the technology of artificially controlling environmental conditions and cultivating useful microorganisms, animal and plant cells on a large scale with high density. It is the basis for a large number of new cells obtained by gene recombination, cell fusion and other technologies in industrial production such as food and medicine.   Cell factories   are commonly used consumables in large-scale cell culture. The common layers of cell factories include 1 layer, 2 layers, 5 layers, 10 layers, 40 layers, etc., which are mainly suitable for the culture of adherent cells. To expand its training scale, it can be achieved by increasing the number of layers. This training method can maximize the utilization of large space, save plant space, and save manpower and material resources. In industry, cell factories are widely used in mass production of vaccines, monoclonal antibodies or the pharmaceutical industry. Common animal cells that have b...

The   cell factory   is one of the commonly used consumables in the field of biological research, suitable for industrial mass production and large-scale cell culture, such as vaccines, monoclonal antibodies or the pharmaceutical industry. Cells are very sensitive to the environment, so sterilization of consumables such as cell factories is an essential process before use. Commonly used sterilization methods include ethylene oxide sterilization, high pressure steam sterilization, irradiation sterilization, etc. The sterilization method of the cell factory selects a relatively special electron beam sterilization, which belongs to one of the irradiation sterilization methods. Electron beam sterilization is the use of electron beam rays to destroy the DNA of product microorganisms, so as to effectively inhibit the reproduction of microorganisms, disinfect and sterilize, and prevent mildew, insects, and preservatives. This sterilization method has the following characteristics: 1....