LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

The   cell factory   is a consumable that is often used for adherent cell culture. During the experiment, we need to grasp the growth state of the cells in real time and make relevant adjustments according to the specific situation. Cell viability can be detected by MTT method to determine cell growth. MTT method, also known as MTT colorimetric method, is a method for detecting cell survival and growth. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan and deposit in cells, while dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value can be measured by enzyme-linked immunosorbent assay at 570nm wavelength, which can indirectly reflect the number of living cells. Within a certain cell number range, the amount of MTT crystal formation is proportional to the cell number. This method has been wide...

Cell factories   occupy an important position in large-scale cell culture, mainly used in pharmaceuticals, vaccine production, cell therapy and other fields. Cells are more sensitive to the production environment, so the requirements for consumables are also relatively high. Biological detection is a very important basic detection, mainly including the following items: Cytotoxicity test: Pure cell-killing events caused by cells or chemicals that do not rely on apoptosis or necrosis as cell death mechanisms. Through this test, it can be judged whether the raw materials of the cell factory contain substances that are not conducive to cell growth. Sensitization test: chemical sensitizers act on the body through various ways, and can make immune cells in the body produce specific immune responses to them. damage, showing obvious symptoms and signs. Through this test, it can be judged whether the test substance is an allergen and the sensitization intensity. Hemolysis test: Hemolysis te...

  Nanobacteria is often encountered in cell culture. After the cells are contaminated, the adherent growth will not be affected in a short period of time, and the medium will not quickly turn yellow or turbid like bacteria and fungi. So, what to do if there is Nanobacteria  contamination in the  cell factory ? There is no clear conclusion about Nanobacteria, and it is generally believed that the black matter is the debris produced by the deterioration and death of cells due to various reasons. It can penetrate the filter membrane or spread through the air. It is black dots at low magnification, and black bugs can be seen swimming around at high magnification. The culture medium is also not muddy, generally it will not be too affected, and the cells can still be used. Usually, the cells grow well, and the observed movement does not increase significantly, and there is no obvious change in the color and transparency of the culture medium. Similar phenomena can be found in t...

Mycoplasma contamination is a very common problem when culturing cells in   cell factories . Unlike other contaminations, mycoplasma-contaminated cells generally do not become cloudy, so it is difficult to judge with the naked eye. There are four ways to determine whether cells are contaminated with mycoplasma: 1. DNA fluorescence staining The DNA fluorescence staining method is based on the principle that the fluorescent dye Hoechst33258 can bind to the AT base-rich region in the DNA of mycoplasma. Fluorescent dots are mycoplasma DNA rich in AT base regions. 2. PCR technology The specific primers are designed according to the conserved sequences in the Mycoplasma genome, the nucleic acid of the sample to be tested is amplified, and the diagnosis is made by analyzing the size of the amplified product. PCR detection technology is used for the detection of mycoplasma contamination, with short cycle, high sensitivity, good specificity, simple operation, and can detect a large number o...

Mycoplasma contamination is a frequently encountered problem in basic research and industrial production. When using   cell factories   for cell culture or large-scale industrial production, mycoplasma contamination is also very troublesome. So, what to do in this situation? Mycoplasma is a microorganism whose size is between bacteria and virus (minimum diameter 0.2um) and lives independently. After mycoplasma contaminates cells, the culture medium may not become cloudy. It is relieved by passage and medium exchange, so it is easy to be ignored. But some serious cases can cause slow cell proliferation and even fall off from the cell factory. If mycoplasma contamination occurs, for non-important cells, such as cells in culture, cells of WCB, etc., the culture and the used medium can be inactivated and discarded. For more important cells, MRA treatment, drug-assisted warming treatment, use of mycoplasma-specific serum, inoculation and sterilization in animals, macrophage phagocy...

  Bacterial contamination is a common source of contamination when using   cell factories   to culture cells, including Escherichia coli, Staphylococcus albus, Pseudomonas, etc. What should be done if the cells are contaminated by bacteria? Judgment of pollution: Bacterial pollution is different from other pollution sources. Once the cells in the cell factory are polluted, it is easy to detect. In most cases, the culture solution turns yellow in a short time, indicating that a large amount of acidic substances are produced, and obvious turbidity occurs; The culture medium was not mixed at first, but after a little shaking, many turbid substances floated. Observed under an inverted microscope, it can be seen that there are a large number of spherical particles floating in the culture medium, and sometimes a large number of bacteria exist on and around the cell surface, and the cells stop growing and show poisoning. When necessary, a small amount of culture medium smear can...

Mold contamination is one of the things that researchers are very worried about in cell culture experiments, often leading to project delays and the loss of scarce cells. So how should mold contamination in   cell factories   be dealt with to reduce losses? Judgment of contamination: There are many types of fungi, most of which contaminate cells are Aspergillus, Candida albicans, yeast, black mold, spore fungi, etc. After mold contamination, light yellow or white floating objects are often formed in the culture medium, which are visible to the naked eye and can be easily detected. The culture medium generally remains cloudy in a short period of time. Under an inverted microscope, filamentous, dendritic or tubular hyphae that crisscross the cells can be seen, floating in the culture medium. Many hyphae can be seen in a chain-like arrangement of strains under high magnification. Candida and yeast strains are oval in shape and grow scattered on and around cells. Sometimes hyphae ...