Application of media bottle in cell culture medium

 Media bottle is a container for storing serum, which is generally made of PET or PETG material by injection stretch blowing process. The medium is the nutrient required for cell growth. According to its source, it is divided into synthetic medium and natural medium, both of which can be stored in media bottles.

Natural medium:

The most commonly used natural medium is serum, basically calf serum. Serum contains a variety of cell growth factors, adhesion-promoting factors and their multi-active substances. When used in combination with synthetic medium, cells can proliferate and grow smoothly. Serum needs to be stored in special media bottles at -5°C to -20°C.

Synthetic medium:

Synthetic medium is strictly formulated according to the type and quantity of substances required by cells. There are many types and known components, which facilitates the control of experimental conditions. Contains carbohydrates, amino acids, lipids, inorganic salts, vitamins, trace elements and cell growth factors. However, compared with the natural medium, some natural unknown components cannot be replaced by known chemical components. Therefore, the basic synthetic medium used in cell culture must also add a certain amount of natural medium components to overcome the synthetic culture. The most common practice is to add calf serum.

The shape of the media bottle is square, easy to grasp, resistant to most acid and alkali corrosion, the embrittlement temperature is -70 °C, and it still has a certain toughness at -30 °C. It is a good packaging container, both natural and synthetic media can be stored in media bottle.

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Material features of PETG media bottles

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  PETG media bottles   are a special packaging for storing various media, reagents, serum and other solutions, and it is also a product that scientific researchers have more contact with. The wide range of applications is mainly due to the superior properties of its materials. PETG is a transparent plastic that belongs to the non-crystalline copolyester. Serum needs to be stored in a low temperature environment. The use range of this material is -80°C to 60°C, which makes PETG media bottles fully meet the storage needs of serum. In addition, it has the following features: 1. High transparency, the light transmittance reaches 90%, which can reach the transparency of plexiglass; 2. It has stronger rigidity and hardness, excellent scratch resistance, impact resistance and toughness, which is close to or even exceeds that of polycarbonate (PC), and is suitable for injection molding, extrusion molding and extrusion blowing processing; 3. In terms of chemical resistance, oil resistance, weat
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What are the common specifications of serum bottles

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  In biological laboratories, we often have to store various solutions and reagents. Thanks to the advantages of good performance, transparency, and temperature resistance, serum bottles have become "frequent visitors" in the laboratory. As a commonly used storage container, what are its common specifications?                      FuDau 60ml-Square-PETG-PET-Media-Bottles Common materials for serum bottles include PETG and PET, both of which have very good temperature resistance and transparency. In comparison, bottles made of PETG have more advantages in terms of barrier properties. If you want to pursue a better storage state of the solution, it is recommended to use a bottle made of PETG. There is a molded scale on the body of the bottle, we can clearly observe the capacity and state of the solution. The common specifications of this kind of container include 5ml/10ml/30ml/60ml/125ml/250ml/500ml/1000ml, etc., which are mostly used to store serum, culture medium, buffer, dis
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Cell factory requirements for production raw materials

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Cell factories   are mainly used for large-scale cell culture. Cells have high requirements on the environment, such as temperature, humidity, osmotic pressure, pH value, etc. Therefore, this kind of   cell   consumables also has high requirements on raw materials. The first is transparency. In the process of cell culture, it is necessary to grasp the growth state of the cells at any time, and make corresponding adjustments according to the cell proliferation. Therefore, the raw materials of the cell factory must meet the requirements of transparency, so that it is convenient for researchers to observe the growth of cells. The second is the compatibility of materials. The raw materials of this consumable generally meet the requirements of USP Class VI medical polymer materials. Medical polymer materials refer to polymer materials used to manufacture human internal organs, external organs, pharmaceutical dosage forms and medical devices, and their sources include natural biopolymer mate
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