The solution required for cell factory culture technology - digestion solution

Cell Factory is a multi-layered cell culture consumable suitable for large-scale cell culture. In addition to a specific environment for cell culture, digestive juice is also an essential solution.

In the in vitro culture of tissue cells and the dispersion of tissue cells in primary cell culture (the tissue block is prepared into a single cell suspension) as well as in subculture, the digestion and dispersion of adherent growing cells should use tissue cell digestion solution. The digestion solution used in cell factory culture technology is mainly trypsin, EDTA, etc.

The main components of the cell membrane and the adhesion between cells are lipoproteins and polysaccharides. Trypsin in trypsin can convert proteins into peptones, and pancreatic lipase can decompose fat into glycerol and fatty acids, thereby separating cells. Cells are lysed. The function of the digestion solution is mainly to hydrolyze the intercellular proteins (such as extracellular matrix), to disperse the tissue or adherent cells into single cells, and to make a cell suspension for further experiments.

Digestive enzymes play an important role in the culture of cell factories, but it should be noted that different cells have different responses to pancreatic enzymes. When the pH value is 8.0 and the temperature is 37 °C, the pancreatic enzyme solution has the strongest effect. , the concentration, temperature and time should be well controlled when using, so as to avoid excessive digestion affecting the adherence and growth of cells.