LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  250ml Square Media Bottles   Sufficient inventory, Short Delivery Time, New Price, High Cost Performance! Your Best Choice! If you want to get free samples and any other question, please leave a message or send an email to contact us! 250ml Square Media Bottles are mainly used for storing and sampling active pharmaceutical ingredients, bulk intermediates, and also for preparing, storing buffers, culture.solutions or long-term storage of pH sensitive liquids. It can be used to store reagents but cannot be used as heating vessels.  Features     Type: 250/500/1000ml. Material:PET/PETG Production Environment:C-GMP  Sterilization:60co-γ irradiation SAL=10, Non-Pyrogenic, Dnase & Rnase free. The bottle body is square, easy to hold, easy to store. The surface is smooth and shiny, easy to clean, not easy to leave residue. Good chemical resistence  Low temperature resistence: -70  Product Number Specification Textures Neck D IA (mm) High(mm)   PCS/pack PCS/box C080250 250ml PET 35.4 140(W

  Fudau T75 TC treated falcon   adherent cell culture flasks   use polystyrene (PS) raw materials, ultra-vacuum plasma surface modification(TC treated) process to enhance the adherence performance of the cells, providing more conducive growth environment to the cells . Product Number Treated Size(cm ²） Cultivation Area(cm ²） Cap Pack Case C000025 TC Treated 25 25 Sealed Cap 10 20 C001025 TC Treated 25 25 Vent Cap 10 20 C000075 TC Treated 75 75 Sealed Cap 5 20 C001075 TC Treated 75 75 Vent Cap 5 20 C000175 TC Treated 175 175 Sealed Cap 5 8 C001175 TC Treated 175 175 Vent Cap 5 8 C000225 TC Treated 225 225 Sealed Cap 5 5 C001225 TC Treated 225 225 Vent Cap 5 5  Features    All production process is strictly controlled, through automatic equipment in accordance with cGMP standards in the C-level purification workshop .No personnel contact, No pyrogen, no animal-derived ingredients.Good product consistency PS Material, After TC treated,Provides super strong cell adhesion performance. t75 t

  The  cell culture flask   is a special cell culture consumable with a square and wide bottleneck design. According to the different needs of use, this consumable is divided into 25cm2, 75cm2, 175cm2, 225cm2 and other specifications to meet the different needs of experimenters. Small size: The size of the cell refers to the culture area of ​​the cell culture flask. The 25cm2 and 75cm2 bottles are mainly used for cell recovery and small-scale expansion. Cell resuscitation is a biological term that refers to the process of re-cultivating cells frozen in liquid nitrogen or in a refrigerator at -70°C to restore growth. Cell expansion refers to expanding and reculturing existing cells. Large size: 175cm2 and 225cm are mainly suitable for medium-scale cell culture and eukaryotic protein expression. When used for cell culture, it usually refers to adherent cells, and some suspension cells also use this consumable. Eukaryotic protein expression is a commonly used method for expressing recombi

  Cell passage refers to the process of dividing the culture into small parts, re-seeding in another culture vessel, and then culturing.   Cell culture flasks   are a common consumable in the process of passaging, so what are the specific steps of cell passaging? 1. Take out the cell culture flasks from the incubator, spray 75% alcohol on the surface of the bottle with an alcohol spray can, and transfer it to the ultra-clean table. 2. Open the lid, gently aspirate the old culture medium, add an appropriate amount of PBS buffer to wash 1-2 times with a Pasteur pipette, and discard the PBS buffer. 3. Use a pipette to add 1-2ml of pancreatin and shake the "cross" to make pancreatin fully contact the cells. 4. Transfer the cell culture flask with trypsin to the inverted microscope stage and observe the cell digestion under the microscope. When the cells become round and fall off in large quantities, prepare to terminate the digestion. Spray the surface of the bottle with 75% alco

  Lysogenic bacteria refer to bacteria that have prophages integrated on their chromosomes and can grow and reproduce normally without being lysed. Mild bacteriophages have been widely used in the fields of transduction, genetic engineering vectors, and molecular biology. The   cell culture flask   can be used to check the lysogenicity of bacteria. The specific operation method is as follows: 1. Culture of lysogenic bacteria: Inoculate E. coli 225 (λ) activated by the LB slope into a 250ml cell culture flask containing 20ml LB culture solution, culture with shaking at 30°C for 16 hours, and then take 2ml bacterial suspension from it and connect it to another In a 250ml Erlenmeyer flask containing 20ml of LB broth, culture with shaking at 37°C for 2h to logarithmic phase. 2. Exclude free phage: If the strain to be tested is Bacillus, it can be made into a spore suspension, and then treated at 80°C for 10 minutes to kill the phage that may be adsorbed and free on the surface of the lysog

  The  cell culture flask   is a special cell culture consumable with a square and wide bottleneck design. According to the different needs of use, this consumable is divided into 25cm2, 75cm2, 175cm2, 225cm2 and other specifications to meet the different needs of experimenters. Small size: The size of the cell refers to the culture area of ​​the cell culture flask. The 25cm2 and 75cm2 bottles are mainly used for cell recovery and small-scale expansion. Cell resuscitation is a biological term that refers to the process of re-cultivating cells frozen in liquid nitrogen or in a refrigerator at -70°C to restore growth. Cell expansion refers to expanding and reculturing existing cells. Large size: 175cm2 and 225cm are mainly suitable for medium-scale cell culture and eukaryotic protein expression. When used for cell culture, it usually refers to adherent cells, and some suspension cells also use this consumable. Eukaryotic protein expression is a commonly used method for expressing recombi

  Coliform bacteria refer to a group of gram-negative non-bacillus bacteria that can ferment lactose, produce acid and gas, aerobic and facultative anaerobic. In practical applications, it is often used as an indicator of whether food is contaminated by feces. Among all the detection methods, the use of   cell culture flasks   for detection is the more commonly used one. The operation steps are as follows: 1. Sampling and dilution (1) According to the aseptic method, put 25g (or 25mL) of the test sample in a cell culture flask containing 225mL sterile water (preset appropriate number of glass beads in the flask) or a sterilized mortar, and shake it thoroughly. Or grind to make a uniform dilution of 1:10. It is best to use a sterile homogenizer for solid samples and centrifuge at a speed of 8000 ～ 10000r/min for 1 min to make a 1:10 dilution. (2) Use a 1mL sterile straw to suck 1Ml of 1:10 dilution, pour it into a test tube containing 9mL of sterile water, shake and mix to make a 1:100

Cell culture flasks   play an important role in medium-scale cell and tissue culture in the laboratory. Common specifications include 25cm2, 75cm2, 175cm2, and 225cm2, which can meet the needs of cell culture of different scales. Different from ordinary plastic bottles, the innovations in the design of cell culture flasks are reflected in the following aspects: 1. Bottleneck: The bottleneck of the cell culture flask is designed with torticollis, and the bottom is in the shape of an arc, which is convenient for the export of the culture solution. The bottleneck is relatively wide and there is no dead corner, so that the pipette and the cell scraper can go deep into every corner of the bottle, and the operation is more convenient. 2. Bottle cap: This cell consumable is equipped with two different bottle caps, which are suitable for different culture environments. The sealing cap is in a completely sealed state, and there are no vents on the cap. It is mainly used under carbon dioxide-fre