Material characteristics of cell culture flasks

 Cell culture flasks are commonly used consumables in small-scale cell culture. In order to facilitate the observation of cell growth conditions, polystyrene materials with higher transparency are generally selected for this consumable. So, what are the characteristics of this material?

Polystyrene refers to a polymer synthesized from styrene monomer through radical addition polymerization. It is a colorless and transparent thermoplastic with the following characteristics:

1. Mechanical properties

Polystyrene molecules and their aggregated structure determine that they are rigid and brittle materials, which exhibit brittle fracture under stress.

2. Thermal performance

The embrittlement temperature is about -30°C, the glass transition temperature is 80-105°C, the melting temperature is 140-180°C, and the decomposition temperature is above 300°C. The thermal conductivity is low, ranging from 0.04 to 0.15W/(m·K), and it is hardly changed by temperature, so it has good thermal insulation.

3. Electrical performance

Polystyrene has good electrical properties, with volume resistivity and surface resistivity as high as 1016～1018Ω·cm and 1015～1018Ω, respectively. The dielectric loss tangent value is extremely low, and it is not affected by frequency, environmental temperature, and humidity changes. It is an excellent insulating material.

cell culture flasks

4. Optical performance

Polystyrene has excellent optical properties, with a light transmittance of 88% to 92% and a refractive index of 1.59 to 1.60. It can transmit visible light of all wavelengths. The transparent material is second only to acrylic polymers such as plexiglass in plastics. .

5. Chemical properties

Polystyrene is resistant to various alkalis, salts and aqueous solutions. It is resistant to lower alcohols and certain acids (such as sulfuric acid, phosphoric acid, boric acid, hydrochloric acid with a mass fraction of 10% to 30%, and acetic acid with a mass fraction of 1% to 25%. , Formic acid with a mass fraction of 1% to 90%) is also stable.

The above properties of polystyrene can meet the needs of cell culture flasks. In addition, if it is used for adherent cell culture, the surface of the bottle needs to be specially modified to increase the adherence performance of the cells.

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