LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

In the field of cell culture consumables, choosing the right type of bottle for storing and handling media is crucial. Two common options are   PETG (Polyethylene Terephthalate Glycol) media bottles   and glass media bottles. This article aims to provide a comparative analysis of these two choices, focusing on the advantages of PETG media bottles, our core product. 1.Material Differences: PETG Media Bottles: PETG is a type of plastic known for its durability, chemical resistance, and transparency. PETG media bottles are lightweight and shatterproof, making them an excellent choice for shipping and handling. They also have excellent gas barrier properties, reducing the risk of contamination. Glass Media Bottles: Glass media bottles are known for their traditional appearance and the ability to maintain the integrity of the stored contents. However, they are fragile and prone to breakage, which can lead to safety hazards and contamination concerns. 2.Impact on Cell Culture: PETG Media Bot

  In the world of cell culture, the storage and preservation of cell culture media are critical to maintaining the viability and functionality of cells. At FuDau Bio, we understand the importance of reliable and contamination-free media storage solutions. Our   PETG Media Bottles   have emerged as the optimal choice for this purpose, and this article explores their essential role in cell culture media storage. Unveiling the Potential of PETG Media Bottles Material Excellence: Our PETG Media Bottles are crafted from high-quality polyethylene terephthalate glycol (PETG). This material is chosen for its exceptional attributes, such as transparency, durability, and non-reactivity with cell culture media. This ensures that your stored media remains uncontaminated and retains its quality. Exceptional Transparency: PETG Media Bottles' transparency is an invaluable feature for media storage. It enables you to visually inspect the media without exposing it to potential contaminants. Real-ti

  In today's ever-evolving world of biotechnology, the demand for efficient and reliable tools for vaccine production has never been greater. At FuDau Bio, we take pride in being at the forefront of innovation, providing cutting-edge solutions for cell culture needs. One such innovation is our   Cell Factory , a product that is revolutionizing vaccine manufacturing. The Cell Factory Advantage Higher Yields and Efficiency: Cell Factory is designed to maximize cell culture productivity. With its multi-layered format, it provides a significantly larger surface area for cell growth compared to traditional cell culture vessels. This translates to higher cell yields, allowing for increased vaccine production capacity. Precise Control: Cell Factory offers superior control over culture conditions, including nutrient supply, aeration, and waste removal. This ensures optimal growth conditions for your cells, leading to consistent and reliable vaccine production. Cell Factory Scalability: Whe

  PETG (Polyethylene Terephthalate Glycol) media bottles offer several advantages, making them a popular choice for various applications in laboratories, pharmaceutical companies, and other industries. Here are some of the key advantages of   PETG media bottles : 1.Transparency: PETG is a clear and transparent material, allowing for easy visual inspection of the contents. This is important in laboratory and medical settings where the accurate measurement and observation of liquids is crucial. 2.Chemical Resistance: PETG bottles are resistant to a wide range of chemicals, including many solvents, acids, and bases. This makes them suitable for storing a variety of solutions without fear of material degradation or leaching into the contents. 3.Impact Resistance: PETG is a tough and impact-resistant plastic. It can withstand accidental drops and rough handling, reducing the risk of breakage and potential contamination of the contents. 4.Lightweight: PETG bottles are lightweight, making the

Serum, as a good assistant in cell culture, plays an important role in the growth process of cells. The quality of serum directly affects the results of cell culture. If there is too much precipitation, it will inevitably cause concern. So, how do we avoid precipitation in   petg media bottles ? First of all, we should have a correct understanding of precipitation. When turbid and flocculent substances are found after thawing the serum, there is no need to panic. This is a normal phenomenon. Common deposits include fibrin, calcium phosphate, cholesterol, fatty acid esters, and some proteins. The amount of serum sedimentation is not directly proportional to the quality of the serum. If serum precipitation occurs, don’t worry. Usually, centrifuge at 400-600g for 5 minutes to remove large particle precipitation, and then use the supernatant. PETG Media Bottles Of course, if you still feel uneasy, you can take the following measures to avoid precipitation in the petg media bottles as much

  PETG media bottles   are a plastic storage container with high transparency, which are generally used to store serum, culture medium, buffer solution and other solutions. In order to avoid microbial contamination caused by the packaging, it will be sterilized, and this packaging is mainly sterilized by cobalt 60. Sterilization is to ensure the removal or killing of all bacteria, viruses, fungi and other microorganisms on the PETG media bottles by various physical and chemical means, so as to achieve a sterility assurance level of 10-6, that is, to ensure the survival of microorganisms on an item The probability is only one in a million. Only in this way can microorganisms on the packaging be prevented from causing additional contamination of the contents. PETG Media Bottles Cobalt 60 sterilization is the use of 60Co γ-ray irradiation to act on microorganisms, directly or indirectly destroying the nucleus of microorganisms, thereby killing microorganisms and playing the role of disinf

  As a large-capacity liquid storage container, the   carboy   plays an important role in the field of biopharmaceuticals. It can be used to store sterile liquids such as culture media and cell stock solutions, and can also be used to distribute and mix solutions. It is a safe and reliable liquid storage container. In terms of materials, carboys are generally made of high-quality corrosion-resistant raw materials, such as polypropylene, polycarbonate, etc. These materials have stable chemical properties and can effectively prevent the invasion of external impurities and bacteria, ensuring the purity and stability of the stored liquid. In terms of performance, this kind of container can withstand high temperature and high pressure sterilization, can be reused, and saves costs. At the same time, it has good low-temperature resistance and meets the low-temperature storage needs of different solutions. Carboys - FuDau Additionally, this container is designed with ease of handling and stora

  Once the cells we culture in   cell factory   are contaminated, most of them are difficult to handle. If the contaminated cells are valuable and difficult to obtain again, the following methods can be used to remove them. 1. Use antibiotics Antibiotics are more effective at killing bacteria in cell factories. Combination medication is more effective than medication alone. Preventive medication is more effective than medication after contamination. Preventive medication generally uses a double antibiotic (penicillin 100u/mL plus streptomycin 100μg/mL). After contamination, the cleaning method needs to be 5 to 10 times greater than the usual amount. The drug should be used for 24 to 48 hours after the addition, and then replaced with the usual routine. Culture fluid. This method may be effective in the early stages of contamination. In addition to penicillin and streptomycin, the antibiotics used can also include gentamicin, kanamycin, polymyxin, tetracycline, nystatin, etc. Commonly u

  As a large-capacity liquid storage container, the   carboy   plays an important role in the field of biopharmaceuticals. It can be used to store sterile liquids such as culture media and cell stock solutions, and can also be used to distribute and mix solutions. It is a safe and reliable liquid storage container. In terms of materials, carboys are generally made of high-quality corrosion-resistant raw materials, such as polypropylene, polycarbonate, etc. These materials have stable chemical properties and can effectively prevent the invasion of external impurities and bacteria, ensuring the purity and stability of the stored liquid. In terms of performance, this kind of container can withstand high temperature and high pressure sterilization, can be reused, and saves costs. At the same time, it has good low-temperature resistance and meets the low-temperature storage needs of different solutions. Carboys - FuDau Additionally, this container is designed with ease of handling and stora

  The   carboys   are a large-capacity liquid storage container. Our common specifications are 10L, 20L, etc. It is mainly used to store sterile liquids, distribute solutions and culture media. In biopharmaceutical processes, they are often used to store stock solutions. In the biopharmaceutical process, after the expansion of the cell culture medium is completed, the original solution needs to be harvested for the next step of the process. Since the volume of the original solution after amplification is generally very large, large-capacity packaging such as carboys are used for harvesting. After harvesting, it goes through deep filtration, inactivation, purification and other processes, and then is diluted according to a certain ratio to make vaccines or drugs. Carboys Commonly used materials for carboys include polypropylene and polycarbonate. Both materials have very good chemical stability and can withstand corrosion from most acid and alkali solvents, ensuring that the solution wi

  PETG media bottles   are a special packaging for storing various media, reagents, serum and other solutions, and it is also a product that scientific researchers have more contact with. The wide range of applications is mainly due to the superior properties of its materials. PETG is a transparent plastic that belongs to the non-crystalline copolyester. Serum needs to be stored in a low temperature environment. The use range of this material is -80°C to 60°C, which makes PETG media bottles fully meet the storage needs of serum. In addition, it has the following features: 1. High transparency, the light transmittance reaches 90%, which can reach the transparency of plexiglass; 2. It has stronger rigidity and hardness, excellent scratch resistance, impact resistance and toughness, which is close to or even exceeds that of polycarbonate (PC), and is suitable for injection molding, extrusion molding and extrusion blowing processing; 3. In terms of chemical resistance, oil resistance, weat

  When we use   cell factories   to culture cells, the growth status of the cells is affected by various factors. These factors may seem inconspicuous, but if not paid attention to, they will cause heavy damage to the cells. So, how do small details when using cell factories affect cell growth, and how can we avoid pitfalls when using cell factories to culture cells? 1. Degree of contact between cells Normal cells (diploids) have attachment contact inhibition, and cell growth stops after overgrowth. However, abnormal cells such as tumor cells have weak contact inhibition and can grow to higher cell densities, but some cells overgrow. The damage is difficult to reverse, so it is not recommended to have too high a cell density when culturing cells. Important points: Generally, cells cultured in a cell factory can be passaged when the cell confluence reaches 70-80%. Do not overgrow the cells. Cell Factory Systems 2. Cell growth attachments Adherent cells will adhere to the wall under norm

  PC carboys , or polycarbonate carboys, are commonly used in homebrewing and laboratory settings for the storage and transportation of liquids. Whether or not they can be reused depends on several factors, including their condition, how they've been used, and proper cleaning and maintenance. Here are some considerations for reusing PC carboys: 1.Condition: Inspect the carboy for any signs of damage or wear. If the carboy is cracked, scratched, or has any visible damage, it should not be reused, as these defects can compromise its integrity and potentially lead to leaks or contamination. 2.Previous Contents: Consider what liquids were stored in the carboy previously. Some substances can leave behind residues or odors that are difficult to remove, making the carboy unsuitable for reuse. Chemicals that are particularly harsh or reactive may also degrade the polycarbonate material over time. 3.Cleaning: Proper cleaning is crucial for reusing PC carboys. Thoroughly clean the carboy wit

  When we use cell culture consumables such as   cell factories   to culture cells, we need to perform routine inspections on the cells. Observe the cell morphology and growth, as well as the pH changes of the culture, the presence of pollution, etc. According to the dynamic changes of the cells, change the medium or pass the cells. If any abnormalities are found, timely measures should be taken. Generally, observations will be made from the following four aspects: 1.Cell morphology Cells in good growth status can be seen under a general microscope. The cells are highly transparent, refractive, and have unclear outlines. When cells grow poorly, their outlines are enhanced, vacuoles, lipid droplets, and other granular objects often appear in the cytoplasm. The gaps between cells increase, and the cell shape can become irregular or even lose its original characteristics. Such as epithelial cells becoming epithelial-like cells, etc. 2. Cell growth After the primary culture or passaged cel

  Cell factories , which are used for large-scale production of cells or cellular products, must adhere to strict quality standards to ensure the safety, efficacy, and consistency of the final products. These standards are critical in various fields, including biopharmaceuticals, biotechnology, and regenerative medicine. Here are some of the key quality standards and guidelines that cell factories should follow: 1.Good Manufacturing Practices (GMP): GMP is a set of regulations and guidelines that ensure the consistent quality and safety of pharmaceutical and biopharmaceutical products. Cell factories involved in producing therapeutic cells or cellular products for human use must adhere to GMP standards, including cleanroom requirements, documentation, and quality control procedures. 2.ISO Standards: International Organization for Standardization (ISO) has several standards relevant to cell factory operations, such as ISO 9001 (Quality Management System) and ISO 13485 (Quality Managemen

  Cell factories   are specialized culture vessels used for large-scale production of cells, such as mammalian or microbial cells, for various applications including biopharmaceutical production, vaccine development, and tissue engineering. While cell factories typically do not use caps in the traditional sense, as they are often equipped with specialized closures or systems to ensure a sterile and controlled environment, there are several components that play a crucial role in their operation. Here are some of the important components used in cell factories: 1.Sterile Filters: Cell factories require aeration and gas exchange while maintaining sterility. Sterile filters are essential components that are integrated into the design of cell factories. They allow the exchange of gases, such as oxygen and carbon dioxide, while preventing the entry of contaminants. These filters are typically made of materials that provide both filtration and microbial retention capabilities. 2.Tubing and Co

Operating a cell factory involves the controlled cultivation and manipulation of cells to produce specific products or compounds. Cell factories are commonly used in biotechnology, pharmaceuticals, and industrial processes. Here's a general overview of how to operate a   cell factory : 1.Select the Host Organism: Choose a suitable host organism for your specific application. Common choices include bacteria (e.g., Escherichia coli), yeast (e.g., Saccharomyces cerevisiae), mammalian cells (e.g., Chinese hamster ovary cells), and plant cells. The choice of host organism depends on factors such as the product to be produced, scalability, and the ability to genetically engineer the organism. 2.Genetic Engineering: If necessary, genetically modify the host organism to make it capable of producing the desired product or compound. Introduce or modify genes within the host's genome to enable the production of the target molecule. 3.Cultivation: Grow the genetically modified cells in a c

  In biopharmaceuticals, the storage and transfer of liquids are often involved, and   carboys   provide a lot of convenience for this operation. Its large-capacity design, high temperature and high pressure resistance and other features are favored by many scientific research institutions and pharmaceutical companies. So, can this kind of container be reused? Let’s find out the answer together. The commonly used raw materials for carboys are PP and PC. From the perspective of chemical properties, they both have good corrosion resistance and are resistant to most acid and alkali solvents. This kind of liquid storage container has a large capacity, with common specifications of 10L and 20L, which can meet the needs of large-capacity storage and transfer of liquids, especially in the large-scale culture stage of cells. 10L PC Carboys From the perspective of temperature resistance, both PP and PC materials can withstand high-temperature and high-pressure sterilization. This sterilization