LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  Cell culture flasks   are commonly used tools for adherent cell culture, and cell subculture operations are involved in culturing cells. How should we passage adherent cells? You can follow these steps: 1. Take out the preheated culture solution: take out the preheated culture solution, wipe it with an alcohol cotton ball before putting it into the ultra-clean bench. 2. Take out the cells from the incubator: Pay attention to tighten the bottle cap when taking out the cells from the cell culture bottle, wipe the microscope table with alcohol cotton balls, and then observe the cells under the microscope. 3. Put the culture bottle into the ultra-clean workbench and open the mouth of the bottle, and at the same time burn the mouth on the alcohol lamp for disinfection. 4. Add digestive solution: Carefully suck out the old culture solution, wash (rinse) with PBS, add appropriate amount of digestive solution (trypsin solution), pay attention to the amount of digestive solution to cover the

  As an economical and affordable cell culture tool, the   erlenmeyer shake flask   has a wide range of applications in the fields of biopharmaceuticals, vaccine preparation, cell and gene therapy, etc. Among them, this kind of container is also used in lentivirus transfection. Lentiviral vectors are gene therapy vectors developed on the basis of HIV-1. Different from general retroviral vectors, it has the ability to infect both dividing and non-dividing cells, and is widely used in the study of expressing RNAi. The cell shake flask is mainly used in the experiment of lentivirus transfection of suspension cells. The steps are as follows:                   125ml-Erlenmeyer-Shake-Flasks 1. Add polybrene to 6 μg/ml and an appropriate amount of virus to 2×105/ml suspended cells, and mix well. Incubate at 37°C. Or centrifuge at room temperature at 150g for 4 hours (selected, this step can improve transfection efficiency for some difficult-to-transfect cell lines). 2. (Selected for this step

  Protein expression refers to a molecular biology technology that expresses foreign gene proteins by model organisms such as bacteria, yeast, animal cells or plant cells, and occupies a core position in genetic engineering technology.   Erlenmeyer shake flask   is a consumable often used in protein expression. Protein expression is the process in which genes on DNA are transcribed into RNA and then translated into proteins. Protein expression can express the corresponding (genetic) traits controlled by genes. According to the host cells, protein expression systems can be divided into two categories: prokaryotic expression systems and eukaryotic expression systems. Prokaryotic expression systems include Escherichia coli expression system, Bacillus expression system, Streptomyces expression system. Eukaryotic expression systems include yeast expression systems, insect expression systems, mammalian expression systems and plant cell expression systems.               FuDau 125ml Erlenmeyer

  The   erlenmeyer shake flask   is a triangular cell culture container. It has two different materials: PC and PETG. According to different uses, there are also two types of lids: vent Cap and sealed Cap. Today, we will introduce the two basic functions of this bottle. Cultivation of suspension cells and fungi: The erlenmeyer shake flask is suitable for cultivating cell lines with high requirements for central enterprises. It is also used to cultivate bacteria, fungi and animal and plant cells in suspension. Compared with culture flasks, dishes and spinner flasks, the required It requires less work and is an affordable cell culture tool. It can be used in small-scale process development, step-by-step scale-up and other cultivation stages. The breathable cover has a 0.2μm breathable membrane, which is breathable and impermeable to water, which can effectively prevent the entry of microorganisms, prevent pollution, and ensure gas exchange, so that cells grow well.      FuDau 1000ml Erle