LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  Insect cells are a class of eukaryotic cells mainly extracted from insects. By cultivating the host virus, it can cause insect epidemics, but it is harmless to humans, animals and plants, so it can be used as a virus insecticide and can also produce some medicinal proteins.   Cell culture flask   is a kind of cell culture consumable commonly used for culturing insect cells, so what problems should be paid attention to when culturing insect cells? 1. Temperature and environment: Insect cells should generally be cultured in a non-humidified environment at 27°C. Carbon dioxide exchange is not recommended, so try to choose a closed lid for the cell culture flask. 2. Culture medium: Different cells have different requirements for culture medium. According to the cell growth characteristics, a special medium for insect cells should be selected. 3. Adherent insect cells: When the cell density is less than 20% of the confluent state, cell growth will be inhibited. Healthy cells are harvested

  The cell growth curve is a common method for determining the absolute growth number of cells, an important indicator for determining cell viability, and one of the basic parameters for the biological characteristics of cultured cells. There are many ways to measure the growth curve of cells, and counting in   cell culture flasks   is a common way. FuDau T25 Cell Culture Flasks Generally, after the cells are passaged, they are suspended for a short time and then adhere to the wall, and then spend different incubation periods of different lengths, that is, they enter the exponential growth phase of massive division. After the cells reach saturation density, they stop growing, enter the plateau phase, and then degenerate and die. In order to accurately describe the dynamic changes of cell numbers in the whole process, a typical growth curve can be divided into four parts: a slow-growing latent period, an exponential growth period with a large slope, a plateau-shaped flat-top period, and

  Cell culture can be divided into adherent cells and suspension cells according to the different culture methods. Adherent cell culture needs to use TC-treated  cell culture flasks , so why do  TC-treated ? What is TC-treated? The full name of TC-treated is Tissue culturetreated. TC-treated means that the cell culture flask has undergone surface modification treatment and is suitable for the culture of adherent cells.          FuDau T25 Cell Culture Flasks Why do cell culture flasks need TC-treated? Adherent cells need to have a support surface that can be attached, and rely on the attachment factors secreted by themselves or in the culture medium to grow and reproduce on the surface. Most animal cells are adherent cells. At first, the cell culture flasks on the market were mainly made of glass. The glass itself is hydrophilic, and the surface can be suitable for the growth of adherent cells without any treatment. However, in practical applications, the disadvantages of high cleaning

  Cell culture flasks   are the most commonly used cell culture consumables. They are mainly used for the culture of adherent cells. There are many brands on the market, such as Corning, Thermo Fisher, SPL, etc. Fudau is the new Chinese Brand of cell culture flasks.  then what are the characteristics of it? Here is a brief introduction to you. Complete specifications: Four sizes of 25cm2 /75cm2 /175cm2/225cm2 are available to meet the cultivation needs of different scales. Featured ingredients: High-quality transparent polystyrene (PS) raw material, in line with USP VI medical grade.                  FuDau T25 Cell Culture Flasks Production Environment: The whole production process is in the C-level purification workshop, managed in accordance with the ISO13485 quality system and produced by automated equipment, without direct contact with personnel, with good product consistency and small batch-to-batch difference. special design: With torticollis design, there is no dead angle betwee

  Making cells adhere better is the key to ensuring their normal growth in the later stage. Sometimes we encounter the situation that the cells in the   cell culture flasks   do not adhere well. The following methods can better promote cell adherent growth. 1. Increase the seeding density: cells mainly adhere to the surface of the support through the extracellular matrix secreted by themselves. If the cell adhesion is not good, the number of seeding may be too small to secrete enough extracellular matrix. Seeding density to improve adherence.         FuDau T25 Cell Culture Flasks 2. Increase the adhesion factor: The serum contains the adhesion promoting factor, and the lack of this component in the serum-free culture system will greatly reduce the adhesion effect of the cells. Some serum can be appropriately added to promote cell adhesion. 3. Improve the quality of inoculated cells: the state of the cells themselves will also affect their adherence effect. Make sure that the cells are