LUOYANG FUDAU BIOTECH CO.LTD.--Cell Culture Consumables

  The   cell factory   is a multi-layer structure of cell culture consumables, mainly used for large-scale in vitro cell culture. When culturing cells, a specific environment is required, and the gas environment and pH value are one of the most important conditions. The gas environment that the cell factory refers to when cultivating cells is mainly carbon dioxide and oxygen. Oxygen participates in the cell's tricarboxylic acid cycle to generate energy for cell growth, proliferation and synthesis of various required components. Some cells can obtain energy through glycolysis under hypoxic conditions, but most cells cannot survive under hypoxia. Oxygen tension is usually maintained at slightly below atmospheric conditions, and if the partial pressure of oxygen exceeds the amount of oxygen in the atmosphere, some cells may be detrimental. When using open culture (dish or culture bottle loose-cap culture or culture plate culture), the cells are generally placed in a mixed gas environm

  Cell factories   play an important role in large-scale cell culture in the fields of human vaccines, animal vaccines, cell therapy, and stem cells. Although they are the main consumables for cell culture, they also require the assistance of some accessories during use. Commonly used accessories include the following categories: 1. Sealing cover: There are ventilation holes on the top of the cover, which are mainly used in a carbon dioxide environment. The ventilation holes allow carbon dioxide in the environment to enter the cell factory, creating suitable growth conditions for cell growth. 2. Breathable cover: The cover has no ventilation holes and is mainly used in incubators and greenhouses without carbon dioxide. A sealed cover can prevent the invasion of external bacteria and help create a good growth environment for cell growth. 3. Hose: including general hose and welded hose, welded hose can be heat welded. Mainly used for liquid transfer. 4. Hose clamp: its function is to cla

A   cell factory   is a one-layer or multi-layer structure of cell culture consumables. During the cell culture process, we need to grasp the growth state of the cells in time before proceeding to the next step. The number of viable cells can be detected by trypan blue staining. The specific operation method is as follows: 1. For adherent cells, aspirate the medium, add the digestion solution, and digest in the incubator for an appropriate time. After the cells became round, the digestion was terminated by adding an equal or greater volume of medium than the volume of the digestate. After centrifugation, the cell suspension is made with culture medium; 2. Take 0.5ml trypan blue solution, 0.3ml PBS and 0.2ml cell suspension and mix well. Then the mixture was left for 1-2min. Use a capillary pipette or a 10ul small pipette tip to draw a small amount of the mixture and inject it into the hemocytometer; 3. Count at least 1000 cells and count the number of stained cells; 4. Wash the countin

With the wide application of cell culture in the field of life sciences,   cell factories   are widely used as a cell culture consumable in the fields of vaccine production and preparation, monoclonal antibodies, stem cells and somatic cell therapy. Cell growth has strict requirements on the environment, and suitable osmotic pressure is the basis for cells to maintain a good growth state. Cells in hypertonic or hypotonic solutions immediately shrink, swell, and rupture. Therefore, proper osmotic pressure is one of the important conditions for culturing cells in cell factories. The maintenance of osmotic pressure of mammalian and other animal tissue cells in vitro is mainly related to sodium chloride, but the relationship of osmotic pressure of other dielectrics cannot be ignored. The osmotic pressure is proportional to the number of molecules and ions of the solute in the unit volume of the solvent, and the ion balance in the culture medium is controlled according to a certain proporti

The rapid development of the field of life sciences has continued to expand the demand for cell culture consumables. Among them,   cell factories   for large-scale cell culture are favored by many pharmaceutical companies and vaccine manufacturers. Cell growth requires certain conditions, in addition to a sterile environment, it also requires a suitable temperature. Generally, the suitable temperature for mammalian and avian cells to be cultured in vitro is 37-38°C. Unsuitable ambient temperature will affect the growth of cells. The tolerance of cells to low temperature is stronger than that of high temperature. At low temperature, the metabolic activity and mitotic capacity of cells are reduced. If the temperature is not lower than 0 °C, although the cell metabolism is affected, there is no damage; at 25 to 35 °C, the cells grow at a slow rate. High temperature is not good for cell culture. If the cells are cultured at 39~40°C for 1 hour, they will be damaged to a certain extent, but

Cell factories   are mainly used for large-scale cell culture. Cells have high requirements on the environment, such as temperature, humidity, osmotic pressure, pH value, etc. Therefore, this kind of   cell   consumables also has high requirements on raw materials. The first is transparency. In the process of cell culture, it is necessary to grasp the growth state of the cells at any time, and make corresponding adjustments according to the cell proliferation. Therefore, the raw materials of the cell factory must meet the requirements of transparency, so that it is convenient for researchers to observe the growth of cells. The second is the compatibility of materials. The raw materials of this consumable generally meet the requirements of USP Class VI medical polymer materials. Medical polymer materials refer to polymer materials used to manufacture human internal organs, external organs, pharmaceutical dosage forms and medical devices, and their sources include natural biopolymer mate

Cell factories   are commonly used consumables in large-scale cell culture. Cells have high requirements on the growth environment and require specific temperature, osmotic pressure, gas phase and pH value. Therefore, the cell factory as a cell growth carrier must meet the conditions for cell growth. , generally produced under the C-class standard of the clean workshop. A clean workshop is also called a clean room or a clean room, which refers to the removal of particulates, harmful air, bacteria and other pollutants in the air within a certain space, and the indoor temperature, cleanliness, indoor pressure, and airflow speed. It is a specially designed room with airflow distribution, noise vibration and lighting, and static control within a certain range of requirements. That is to say, no matter how the external air conditions change, the interior can maintain the characteristics of cleanliness, temperature, humidity and pressure that were originally set. The C-level clean room stand

Cell factories   are commonly used consumables in the fields of pharmaceutical work, vaccine development, and monoclonal antibodies. Cell culture requires a specific environment, of which sterility is the basic requirement. So, how does a cell factory achieve sterility requirements? The sterility of cell culture means that its growth environment cannot have the existence of other microorganisms, which will affect the growth and reproduction of cells. To meet such requirements, consumables such as cell factories must also be sterile. First of all, these consumables will be produced in the clean workshop to ensure the sterility of the production environment. Secondly, after the production of the cell factory is completed, it will be subjected to electron beam sterilization treatment to meet the requirements of sterility. Electron beam killing biological bacteria technology is a sterilization technology that applies beta ray irradiation to sterilize. It is a method of killing microorganis

Cell factories   are production devices for large-scale cell culture, which are widely used in the fields of vaccines, modern biotechnology and genetic engineering, stem cells and somatic cell therapy. The cell factory is different from the general cell consumables, the use process is more complicated, and it must be operated according to the established operation method. The operation of dividing the liquid level of each layer of the cell factory is as follows: 1. Pour the culture medium directly into the cell factory; 2. Place the cell factory towards the side with the small opening and balance for a period of time; 3. Turn the cell factory 90 degrees, with the side with the liquid inlet facing up, and let it stand for a period of time, the medium will be automatically and evenly distributed to each layer of chambers; 4. Hold one side of the liquid inlet with both hands, slowly bring down the cell factory, and place it horizontally by programming. Do not hold the edge of the first la

  The rapid development of the pharmaceutical industry has allowed cell culture technology to develop its ambitions. With the help of cell culture consumables such as   cell factories , it plays an important role in industrial mass production, such as vaccines, monoclonal antibodies or the pharmaceutical industry. Correct use can make cell culture work more efficiently, so how should cell factories be used? The use of the cell factory should follow certain methods and steps. Improper operation will affect the later cell culture work. The steps for using the cell factory are as follows: 1. Add the required amount of cell suspension to the cell factory by pouring. The recommended volume for each layer is 200ML. 2. After adding the cell suspension, keep the cell factory on its side, with the side with the liquid addition port facing away from you, and balance for a period of time so that the liquid is evenly distributed to each layer. 3. Rotate 90 degrees counterclockwise, so that the liq